Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1420 and EX28DRAFT_1421 are separated by 285 nucleotides EX28DRAFT_1421 and EX28DRAFT_1422 are separated by 600 nucleotides
EX28DRAFT_1420: EX28DRAFT_1420 - phenylacetate-CoA oxygenase, PaaG subunit, at 1,426,936 to 1,427,865
_1420
EX28DRAFT_1421: EX28DRAFT_1421 - phenylacetic acid degradation protein paaN, at 1,428,151 to 1,430,193
_1421
EX28DRAFT_1422: EX28DRAFT_1422 - Cu2+-containing amine oxidase, at 1,430,794 to 1,433,070
_1422
Position (kb)
1428
1429
1430
1431 Strain fitness (log2 ratio)
-1
0
1 at 1427.572 kb on + strand, within EX28DRAFT_1420 at 1427.586 kb on - strand, within EX28DRAFT_1420 at 1428.007 kb on - strand at 1428.213 kb on + strand at 1428.214 kb on - strand at 1428.288 kb on + strand at 1428.289 kb on + strand at 1428.447 kb on + strand, within EX28DRAFT_1421 at 1428.478 kb on + strand, within EX28DRAFT_1421 at 1428.536 kb on + strand, within EX28DRAFT_1421 at 1428.537 kb on - strand, within EX28DRAFT_1421 at 1429.738 kb on + strand, within EX28DRAFT_1421 at 1429.840 kb on + strand, within EX28DRAFT_1421 at 1429.841 kb on - strand, within EX28DRAFT_1421 at 1429.903 kb on + strand, within EX28DRAFT_1421 at 1429.904 kb on - strand, within EX28DRAFT_1421 at 1429.966 kb on - strand, within EX28DRAFT_1421 at 1429.966 kb on - strand, within EX28DRAFT_1421 at 1429.970 kb on - strand, within EX28DRAFT_1421 at 1430.046 kb on + strand at 1430.046 kb on + strand at 1430.239 kb on - strand at 1430.561 kb on + strand at 1430.569 kb on + strand at 1430.570 kb on - strand at 1430.570 kb on - strand at 1430.570 kb on - strand at 1430.704 kb on - strand at 1430.824 kb on - strand at 1430.902 kb on - strand at 1431.105 kb on + strand, within EX28DRAFT_1422 at 1431.150 kb on - strand, within EX28DRAFT_1422
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,427,572 + EX28DRAFT_1420 0.68 -0.7 1,427,586 - EX28DRAFT_1420 0.70 +0.2 1,428,007 - -0.3 1,428,213 + -0.2 1,428,214 - +0.2 1,428,288 + -0.0 1,428,289 + +0.2 1,428,447 + EX28DRAFT_1421 0.14 -0.1 1,428,478 + EX28DRAFT_1421 0.16 +0.0 1,428,536 + EX28DRAFT_1421 0.19 -0.5 1,428,537 - EX28DRAFT_1421 0.19 -0.5 1,429,738 + EX28DRAFT_1421 0.78 -0.1 1,429,840 + EX28DRAFT_1421 0.83 -0.2 1,429,841 - EX28DRAFT_1421 0.83 -0.7 1,429,903 + EX28DRAFT_1421 0.86 -1.7 1,429,904 - EX28DRAFT_1421 0.86 -0.0 1,429,966 - EX28DRAFT_1421 0.89 +0.3 1,429,966 - EX28DRAFT_1421 0.89 +0.6 1,429,970 - EX28DRAFT_1421 0.89 +0.2 1,430,046 + -1.0 1,430,046 + +0.1 1,430,239 - +0.7 1,430,561 + -0.9 1,430,569 + -0.1 1,430,570 - +0.4 1,430,570 - -0.4 1,430,570 - +0.3 1,430,704 - -0.0 1,430,824 - +1.0 1,430,902 - -0.2 1,431,105 + EX28DRAFT_1422 0.14 -0.3 1,431,150 - EX28DRAFT_1422 0.16 -0.5
Or see this region's nucleotide sequence