Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0810 and EX28DRAFT_0811 are separated by 115 nucleotides EX28DRAFT_0811 and EX28DRAFT_0812 are separated by 3 nucleotides EX28DRAFT_0812 and EX28DRAFT_0813 are separated by 75 nucleotides EX28DRAFT_0813 and EX28DRAFT_0814 are separated by 3 nucleotides
EX28DRAFT_0810: EX28DRAFT_0810 - hypothetical protein, at 814,209 to 814,643
_0810
EX28DRAFT_0811: EX28DRAFT_0811 - hypothetical protein, at 814,759 to 815,274
_0811
EX28DRAFT_0812: EX28DRAFT_0812 - hypothetical protein, at 815,278 to 816,354
_0812
EX28DRAFT_0813: EX28DRAFT_0813 - Uncharacterized conserved protein, at 816,430 to 816,870
_0813
EX28DRAFT_0814: EX28DRAFT_0814 - RHS repeat-associated core domain, at 816,874 to 821,232
_0814
Position (kb) 815
816
817 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 814.641 kb on + strand at 814.694 kb on + strand at 814.716 kb on + strand at 814.716 kb on + strand at 814.716 kb on + strand at 814.716 kb on + strand at 814.800 kb on + strand at 814.955 kb on - strand, within EX28DRAFT_0811 at 814.991 kb on + strand, within EX28DRAFT_0811 at 814.991 kb on + strand, within EX28DRAFT_0811 at 814.991 kb on + strand, within EX28DRAFT_0811 at 815.042 kb on - strand, within EX28DRAFT_0811 at 815.043 kb on + strand, within EX28DRAFT_0811 at 815.043 kb on + strand, within EX28DRAFT_0811 at 815.043 kb on + strand, within EX28DRAFT_0811 at 815.044 kb on - strand, within EX28DRAFT_0811 at 815.182 kb on + strand, within EX28DRAFT_0811 at 815.303 kb on + strand at 815.366 kb on - strand at 815.366 kb on - strand at 815.447 kb on - strand, within EX28DRAFT_0812 at 815.620 kb on - strand, within EX28DRAFT_0812 at 815.641 kb on - strand, within EX28DRAFT_0812 at 815.644 kb on - strand, within EX28DRAFT_0812 at 815.644 kb on - strand, within EX28DRAFT_0812 at 815.655 kb on + strand, within EX28DRAFT_0812 at 815.656 kb on - strand, within EX28DRAFT_0812 at 815.818 kb on + strand, within EX28DRAFT_0812 at 815.818 kb on + strand, within EX28DRAFT_0812 at 815.819 kb on - strand, within EX28DRAFT_0812 at 815.819 kb on - strand, within EX28DRAFT_0812 at 815.819 kb on - strand, within EX28DRAFT_0812 at 815.824 kb on + strand, within EX28DRAFT_0812 at 815.824 kb on + strand, within EX28DRAFT_0812 at 815.824 kb on + strand, within EX28DRAFT_0812 at 815.824 kb on + strand, within EX28DRAFT_0812 at 815.825 kb on - strand, within EX28DRAFT_0812 at 815.826 kb on + strand, within EX28DRAFT_0812 at 815.849 kb on + strand, within EX28DRAFT_0812 at 815.886 kb on + strand, within EX28DRAFT_0812 at 815.886 kb on + strand, within EX28DRAFT_0812 at 815.911 kb on - strand, within EX28DRAFT_0812 at 815.964 kb on - strand, within EX28DRAFT_0812 at 815.964 kb on - strand, within EX28DRAFT_0812 at 815.990 kb on + strand, within EX28DRAFT_0812 at 815.994 kb on + strand, within EX28DRAFT_0812 at 816.037 kb on - strand, within EX28DRAFT_0812 at 816.037 kb on - strand, within EX28DRAFT_0812 at 816.037 kb on - strand, within EX28DRAFT_0812 at 816.042 kb on - strand, within EX28DRAFT_0812 at 816.042 kb on - strand, within EX28DRAFT_0812 at 816.187 kb on - strand, within EX28DRAFT_0812 at 816.187 kb on - strand, within EX28DRAFT_0812 at 816.247 kb on + strand at 816.271 kb on - strand at 816.281 kb on - strand at 816.323 kb on + strand at 816.352 kb on + strand at 816.356 kb on - strand at 816.407 kb on + strand at 816.410 kb on + strand at 816.429 kb on + strand at 816.437 kb on - strand at 816.501 kb on + strand, within EX28DRAFT_0813 at 816.502 kb on - strand, within EX28DRAFT_0813 at 816.647 kb on + strand, within EX28DRAFT_0813 at 816.832 kb on - strand at 816.868 kb on + strand at 816.868 kb on + strand at 817.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 814,641 + +0.5 814,694 + -1.0 814,716 + -0.4 814,716 + -1.5 814,716 + +0.8 814,716 + -2.8 814,800 + -0.4 814,955 - EX28DRAFT_0811 0.38 -0.2 814,991 + EX28DRAFT_0811 0.45 +0.1 814,991 + EX28DRAFT_0811 0.45 -0.5 814,991 + EX28DRAFT_0811 0.45 +0.0 815,042 - EX28DRAFT_0811 0.55 +0.2 815,043 + EX28DRAFT_0811 0.55 +0.6 815,043 + EX28DRAFT_0811 0.55 +0.1 815,043 + EX28DRAFT_0811 0.55 +0.1 815,044 - EX28DRAFT_0811 0.55 -0.1 815,182 + EX28DRAFT_0811 0.82 +0.5 815,303 + -0.3 815,366 - -0.4 815,366 - +0.8 815,447 - EX28DRAFT_0812 0.16 +1.8 815,620 - EX28DRAFT_0812 0.32 -0.2 815,641 - EX28DRAFT_0812 0.34 -0.7 815,644 - EX28DRAFT_0812 0.34 -0.1 815,644 - EX28DRAFT_0812 0.34 -1.4 815,655 + EX28DRAFT_0812 0.35 +0.2 815,656 - EX28DRAFT_0812 0.35 -0.0 815,818 + EX28DRAFT_0812 0.50 -0.8 815,818 + EX28DRAFT_0812 0.50 +0.2 815,819 - EX28DRAFT_0812 0.50 +0.5 815,819 - EX28DRAFT_0812 0.50 +0.2 815,819 - EX28DRAFT_0812 0.50 -0.6 815,824 + EX28DRAFT_0812 0.51 -0.4 815,824 + EX28DRAFT_0812 0.51 +0.5 815,824 + EX28DRAFT_0812 0.51 +0.2 815,824 + EX28DRAFT_0812 0.51 +0.2 815,825 - EX28DRAFT_0812 0.51 -0.5 815,826 + EX28DRAFT_0812 0.51 -0.3 815,849 + EX28DRAFT_0812 0.53 -1.4 815,886 + EX28DRAFT_0812 0.56 +0.1 815,886 + EX28DRAFT_0812 0.56 -0.7 815,911 - EX28DRAFT_0812 0.59 +0.9 815,964 - EX28DRAFT_0812 0.64 -0.3 815,964 - EX28DRAFT_0812 0.64 -0.0 815,990 + EX28DRAFT_0812 0.66 -0.0 815,994 + EX28DRAFT_0812 0.66 -0.4 816,037 - EX28DRAFT_0812 0.70 +0.1 816,037 - EX28DRAFT_0812 0.70 -0.1 816,037 - EX28DRAFT_0812 0.70 +0.0 816,042 - EX28DRAFT_0812 0.71 +0.4 816,042 - EX28DRAFT_0812 0.71 -0.6 816,187 - EX28DRAFT_0812 0.84 -0.3 816,187 - EX28DRAFT_0812 0.84 +0.8 816,247 + -0.1 816,271 - +0.3 816,281 - +0.0 816,323 + +1.6 816,352 + -0.0 816,356 - +0.6 816,407 + +0.3 816,410 + +0.2 816,429 + -0.5 816,437 - -0.2 816,501 + EX28DRAFT_0813 0.16 +0.2 816,502 - EX28DRAFT_0813 0.16 +0.5 816,647 + EX28DRAFT_0813 0.49 +0.1 816,832 - +1.2 816,868 + +0.7 816,868 + -0.6 817,151 + +0.4
Or see this region's nucleotide sequence