Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0298 and EX28DRAFT_0299 are separated by 48 nucleotides EX28DRAFT_0299 and EX28DRAFT_0300 are separated by 44 nucleotides EX28DRAFT_0300 and EX28DRAFT_0301 are separated by 13 nucleotides EX28DRAFT_0301 and EX28DRAFT_0302 are separated by 75 nucleotides
EX28DRAFT_0298: EX28DRAFT_0298 - ABC-type histidine transport system, ATPase component, at 260,069 to 260,842
_0298
EX28DRAFT_0299: EX28DRAFT_0299 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 260,891 to 261,400
_0299
EX28DRAFT_0300: EX28DRAFT_0300 - TIGR01777 family protein, at 261,445 to 262,338
_0300
EX28DRAFT_0301: EX28DRAFT_0301 - FolB domain, at 262,352 to 262,720
_0301
EX28DRAFT_0302: EX28DRAFT_0302 - Glutathione S-transferase, at 262,796 to 263,425
_0302
Position (kb) 261
262
263 Strain fitness (log2 ratio) -1
0
1 at 260.472 kb on - strand, within EX28DRAFT_0298 at 260.840 kb on + strand at 260.841 kb on - strand at 261.136 kb on + strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.438 kb on - strand at 261.443 kb on - strand at 261.443 kb on - strand at 261.454 kb on + strand at 261.454 kb on + strand at 261.476 kb on - strand at 261.476 kb on - strand at 261.545 kb on - strand, within EX28DRAFT_0300 at 261.652 kb on - strand, within EX28DRAFT_0300 at 261.729 kb on + strand, within EX28DRAFT_0300 at 262.088 kb on - strand, within EX28DRAFT_0300 at 262.147 kb on - strand, within EX28DRAFT_0300 at 262.339 kb on - strand at 262.339 kb on - strand at 262.353 kb on + strand at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.437 kb on + strand, within EX28DRAFT_0301 at 262.438 kb on - strand, within EX28DRAFT_0301 at 262.438 kb on - strand, within EX28DRAFT_0301 at 262.699 kb on + strand at 262.742 kb on + strand at 262.758 kb on + strand at 262.758 kb on + strand at 262.759 kb on - strand at 262.759 kb on - strand at 262.950 kb on + strand, within EX28DRAFT_0302 at 263.005 kb on - strand, within EX28DRAFT_0302 at 263.194 kb on - strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.327 kb on + strand, within EX28DRAFT_0302 at 263.328 kb on - strand, within EX28DRAFT_0302 at 263.328 kb on - strand, within EX28DRAFT_0302 at 263.329 kb on + strand, within EX28DRAFT_0302 at 263.329 kb on + strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302 at 263.330 kb on - strand, within EX28DRAFT_0302
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 260,472 - EX28DRAFT_0298 0.52 -0.9 260,840 + +0.2 260,841 - -0.7 261,136 + EX28DRAFT_0299 0.48 -0.0 261,137 - EX28DRAFT_0299 0.48 -0.4 261,137 - EX28DRAFT_0299 0.48 +1.4 261,137 - EX28DRAFT_0299 0.48 +0.0 261,137 - EX28DRAFT_0299 0.48 +0.3 261,438 - -1.2 261,443 - +0.4 261,443 - +0.4 261,454 + -0.2 261,454 + -0.9 261,476 - +0.2 261,476 - +0.7 261,545 - EX28DRAFT_0300 0.11 +0.3 261,652 - EX28DRAFT_0300 0.23 +1.0 261,729 + EX28DRAFT_0300 0.32 -0.3 262,088 - EX28DRAFT_0300 0.72 -0.2 262,147 - EX28DRAFT_0300 0.79 -0.9 262,339 - +1.0 262,339 - +0.5 262,353 + -0.4 262,437 + EX28DRAFT_0301 0.23 +0.4 262,437 + EX28DRAFT_0301 0.23 +0.8 262,437 + EX28DRAFT_0301 0.23 +0.6 262,437 + EX28DRAFT_0301 0.23 +0.3 262,438 - EX28DRAFT_0301 0.23 +0.7 262,438 - EX28DRAFT_0301 0.23 +0.6 262,699 + -0.3 262,742 + +0.1 262,758 + +0.3 262,758 + -0.7 262,759 - +0.1 262,759 - -0.8 262,950 + EX28DRAFT_0302 0.24 -0.1 263,005 - EX28DRAFT_0302 0.33 -0.9 263,194 - EX28DRAFT_0302 0.63 -1.2 263,327 + EX28DRAFT_0302 0.84 -1.7 263,327 + EX28DRAFT_0302 0.84 +1.1 263,327 + EX28DRAFT_0302 0.84 +0.2 263,327 + EX28DRAFT_0302 0.84 -0.0 263,328 - EX28DRAFT_0302 0.84 -0.9 263,328 - EX28DRAFT_0302 0.84 +0.4 263,329 + EX28DRAFT_0302 0.85 +0.1 263,329 + EX28DRAFT_0302 0.85 -0.1 263,330 - EX28DRAFT_0302 0.85 +0.3 263,330 - EX28DRAFT_0302 0.85 +0.2 263,330 - EX28DRAFT_0302 0.85 -0.2
Or see this region's nucleotide sequence