Experiment: carbonsource_succinate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13865 and AMB_RS13870 are separated by 190 nucleotides AMB_RS13870 and AMB_RS13875 are separated by 92 nucleotides AMB_RS13875 and AMB_RS13880 overlap by 4 nucleotides AMB_RS13880 and AMB_RS13885 overlap by 4 nucleotides
AMB_RS13865: AMB_RS13865 - biotin synthase, at 2,943,285 to 2,944,265
_RS13865
AMB_RS13870: AMB_RS13870 - DNA gyrase inhibitor, at 2,944,456 to 2,944,989
_RS13870
AMB_RS13875: AMB_RS13875 - 8-amino-7-oxononanoate synthase, at 2,945,082 to 2,946,260
_RS13875
AMB_RS13880: AMB_RS13880 - alpha/beta hydrolase, at 2,946,257 to 2,946,895
_RS13880
AMB_RS13885: AMB_RS13885 - methyltransferase domain-containing protein, at 2,946,892 to 2,947,626
_RS13885
Position (kb)
2945
2946
2947 Strain fitness (log2 ratio)
-1
0
1 at 2944.306 kb on - strand at 2944.306 kb on - strand at 2944.497 kb on + strand at 2944.605 kb on - strand, within AMB_RS13870 at 2944.791 kb on + strand, within AMB_RS13870 at 2944.791 kb on + strand, within AMB_RS13870 at 2944.791 kb on + strand, within AMB_RS13870 at 2944.792 kb on - strand, within AMB_RS13870 at 2944.792 kb on - strand, within AMB_RS13870 at 2944.792 kb on - strand, within AMB_RS13870 at 2944.968 kb on + strand at 2944.968 kb on + strand at 2944.969 kb on - strand at 2944.969 kb on - strand at 2944.990 kb on - strand at 2944.990 kb on - strand at 2944.990 kb on - strand at 2944.990 kb on - strand at 2945.067 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbonsource_succinate remove 2,944,306 - -0.0 2,944,306 - -1.4 2,944,497 + -0.3 2,944,605 - AMB_RS13870 0.28 -1.1 2,944,791 + AMB_RS13870 0.63 +0.7 2,944,791 + AMB_RS13870 0.63 +0.9 2,944,791 + AMB_RS13870 0.63 +0.4 2,944,792 - AMB_RS13870 0.63 -1.4 2,944,792 - AMB_RS13870 0.63 +1.4 2,944,792 - AMB_RS13870 0.63 -0.2 2,944,968 + +0.1 2,944,968 + +1.1 2,944,969 - -0.3 2,944,969 - +0.0 2,944,990 - +1.0 2,944,990 - -0.3 2,944,990 - -0.3 2,944,990 - +0.7 2,945,067 + -0.1
Or see this region's nucleotide sequence