Experiment: carbonsource_succinate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12390 and AMB_RS12395 overlap by 4 nucleotides AMB_RS12395 and AMB_RS12400 are separated by 20 nucleotides AMB_RS12400 and AMB_RS12405 are separated by 91 nucleotides
AMB_RS12390: AMB_RS12390 - transcription-repair coupling factor, at 2,642,039 to 2,645,518
_RS12390
AMB_RS12395: AMB_RS12395 - succinate dehydrogenase assembly factor 2, at 2,645,515 to 2,645,832
_RS12395
AMB_RS12400: AMB_RS12400 - ATP-dependent DNA helicase RecG, at 2,645,853 to 2,647,934
_RS12400
AMB_RS12405: AMB_RS12405 - UPF0016 domain-containing protein, at 2,648,026 to 2,648,607
_RS12405
Position (kb)
2645
2646
2647
2648 Strain fitness (log2 ratio)
-2
-1
0
1 at 2644.909 kb on + strand, within AMB_RS12390 at 2644.910 kb on - strand, within AMB_RS12390 at 2644.910 kb on - strand, within AMB_RS12390 at 2644.998 kb on + strand, within AMB_RS12390 at 2645.028 kb on + strand, within AMB_RS12390 at 2645.064 kb on + strand, within AMB_RS12390 at 2645.286 kb on + strand at 2645.287 kb on - strand at 2645.394 kb on - strand at 2645.785 kb on - strand, within AMB_RS12395 at 2645.785 kb on - strand, within AMB_RS12395 at 2645.785 kb on - strand, within AMB_RS12395 at 2645.785 kb on - strand, within AMB_RS12395 at 2645.815 kb on + strand at 2645.816 kb on - strand at 2645.821 kb on - strand at 2645.822 kb on + strand at 2645.823 kb on - strand at 2645.823 kb on - strand at 2645.823 kb on - strand at 2647.921 kb on + strand at 2647.974 kb on + strand at 2647.974 kb on + strand at 2647.975 kb on - strand at 2647.975 kb on - strand at 2647.975 kb on - strand at 2648.100 kb on + strand, within AMB_RS12405 at 2648.100 kb on + strand, within AMB_RS12405 at 2648.608 kb on - strand at 2648.781 kb on + strand at 2648.805 kb on + strand at 2648.805 kb on + strand at 2648.806 kb on - strand at 2648.806 kb on - strand at 2648.836 kb on + strand at 2648.857 kb on + strand at 2648.857 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbonsource_succinate remove 2,644,909 + AMB_RS12390 0.82 -0.5 2,644,910 - AMB_RS12390 0.82 -0.6 2,644,910 - AMB_RS12390 0.82 -0.8 2,644,998 + AMB_RS12390 0.85 +0.2 2,645,028 + AMB_RS12390 0.86 -0.8 2,645,064 + AMB_RS12390 0.87 -0.6 2,645,286 + -1.7 2,645,287 - -0.1 2,645,394 - -0.8 2,645,785 - AMB_RS12395 0.85 -1.3 2,645,785 - AMB_RS12395 0.85 -1.2 2,645,785 - AMB_RS12395 0.85 -0.0 2,645,785 - AMB_RS12395 0.85 -2.2 2,645,815 + -0.6 2,645,816 - -0.0 2,645,821 - -0.3 2,645,822 + -1.0 2,645,823 - -0.1 2,645,823 - +0.0 2,645,823 - -0.0 2,647,921 + +1.0 2,647,974 + -0.2 2,647,974 + +0.3 2,647,975 - -0.2 2,647,975 - +0.1 2,647,975 - +0.2 2,648,100 + AMB_RS12405 0.13 -0.1 2,648,100 + AMB_RS12405 0.13 -0.4 2,648,608 - -0.3 2,648,781 + -1.0 2,648,805 + -0.5 2,648,805 + +0.1 2,648,806 - -1.2 2,648,806 - -0.4 2,648,836 + -0.6 2,648,857 + -0.1 2,648,857 + -0.7
Or see this region's nucleotide sequence