Strain Fitness in Escherichia coli BW25113 around b3384

Experiment: D-Trehalose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhfZ and trpS are separated by 289 nucleotidestrpS and gph overlap by 8 nucleotidesgph and rpe overlap by 8 nucleotides b3383: yhfZ - hypothetical protein (NCBI), at 3,509,461 to 3,510,366 yhfZ b3384: trpS - tryptophanyl-tRNA synthetase (NCBI), at 3,510,656 to 3,511,660 trpS b3385: gph - phosphoglycolate phosphatase (NCBI), at 3,511,653 to 3,512,411 gph b3386: rpe - ribulose-phosphate 3-epimerase (NCBI), at 3,512,404 to 3,513,081 rpe Position (kb) 3510 3511 3512Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3509.723 kb on + strand, within yhfZat 3509.803 kb on + strand, within yhfZat 3509.803 kb on + strand, within yhfZat 3509.875 kb on - strand, within yhfZat 3509.888 kb on - strand, within yhfZat 3509.888 kb on - strand, within yhfZat 3509.926 kb on - strand, within yhfZat 3509.966 kb on + strand, within yhfZat 3509.966 kb on + strand, within yhfZat 3510.024 kb on - strand, within yhfZat 3510.058 kb on - strand, within yhfZat 3510.062 kb on + strand, within yhfZat 3510.084 kb on - strand, within yhfZat 3510.098 kb on - strand, within yhfZat 3510.098 kb on - strand, within yhfZat 3510.117 kb on - strand, within yhfZat 3510.124 kb on + strand, within yhfZat 3510.145 kb on - strand, within yhfZat 3510.189 kb on + strand, within yhfZat 3510.189 kb on + strand, within yhfZat 3510.189 kb on + strand, within yhfZat 3510.584 kb on + strandat 3510.584 kb on + strandat 3510.596 kb on + strandat 3510.597 kb on + strandat 3510.602 kb on + strandat 3510.605 kb on + strandat 3510.628 kb on - strandat 3510.635 kb on + strandat 3510.635 kb on + strandat 3511.699 kb on - strandat 3511.714 kb on + strandat 3511.714 kb on + strandat 3511.714 kb on + strandat 3511.793 kb on + strand, within gphat 3511.795 kb on + strand, within gphat 3511.820 kb on + strand, within gphat 3511.825 kb on - strand, within gphat 3511.851 kb on + strand, within gphat 3511.851 kb on + strand, within gphat 3511.856 kb on - strand, within gphat 3511.877 kb on + strand, within gphat 3511.877 kb on + strand, within gphat 3511.887 kb on + strand, within gphat 3511.897 kb on - strand, within gphat 3511.925 kb on - strand, within gphat 3511.950 kb on - strand, within gphat 3511.950 kb on - strand, within gphat 3511.965 kb on + strand, within gphat 3511.965 kb on + strand, within gphat 3511.965 kb on + strand, within gphat 3511.973 kb on - strand, within gphat 3511.975 kb on - strand, within gphat 3511.975 kb on - strand, within gphat 3511.985 kb on - strand, within gphat 3512.007 kb on + strand, within gphat 3512.011 kb on - strand, within gphat 3512.020 kb on - strand, within gphat 3512.021 kb on - strand, within gphat 3512.026 kb on - strand, within gphat 3512.030 kb on + strand, within gphat 3512.038 kb on - strand, within gphat 3512.038 kb on - strand, within gphat 3512.038 kb on - strand, within gphat 3512.104 kb on + strand, within gphat 3512.109 kb on + strand, within gphat 3512.132 kb on + strand, within gphat 3512.140 kb on - strand, within gphat 3512.140 kb on - strand, within gphat 3512.140 kb on - strand, within gphat 3512.166 kb on + strand, within gphat 3512.166 kb on + strand, within gphat 3512.172 kb on - strand, within gphat 3512.177 kb on + strand, within gphat 3512.216 kb on - strand, within gphat 3512.217 kb on + strand, within gphat 3512.218 kb on + strand, within gphat 3512.226 kb on - strand, within gphat 3512.226 kb on - strand, within gphat 3512.235 kb on + strand, within gphat 3512.246 kb on + strand, within gphat 3512.295 kb on + strand, within gphat 3512.295 kb on + strand, within gphat 3512.313 kb on - strand, within gphat 3512.313 kb on - strand, within gphat 3512.345 kb on + strandat 3512.345 kb on + strandat 3512.352 kb on + strandat 3512.358 kb on - strandat 3512.360 kb on - strandat 3512.360 kb on - strandat 3512.368 kb on - strandat 3512.425 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Trehalose (C)
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3,509,723 + yhfZ b3383 0.29 +0.1
3,509,803 + yhfZ b3383 0.38 -0.3
3,509,803 + yhfZ b3383 0.38 +0.2
3,509,875 - yhfZ b3383 0.46 +0.8
3,509,888 - yhfZ b3383 0.47 +0.4
3,509,888 - yhfZ b3383 0.47 -1.9
3,509,926 - yhfZ b3383 0.51 -0.3
3,509,966 + yhfZ b3383 0.56 +0.6
3,509,966 + yhfZ b3383 0.56 +0.1
3,510,024 - yhfZ b3383 0.62 +0.4
3,510,058 - yhfZ b3383 0.66 +0.5
3,510,062 + yhfZ b3383 0.66 -0.0
3,510,084 - yhfZ b3383 0.69 +0.3
3,510,098 - yhfZ b3383 0.70 +0.0
3,510,098 - yhfZ b3383 0.70 +0.7
3,510,117 - yhfZ b3383 0.72 +0.7
3,510,124 + yhfZ b3383 0.73 +0.1
3,510,145 - yhfZ b3383 0.75 +0.6
3,510,189 + yhfZ b3383 0.80 +0.1
3,510,189 + yhfZ b3383 0.80 -2.3
3,510,189 + yhfZ b3383 0.80 -0.9
3,510,584 + +0.1
3,510,584 + -0.1
3,510,596 + -0.0
3,510,597 + +1.0
3,510,602 + +0.0
3,510,605 + -0.5
3,510,628 - -0.1
3,510,635 + -0.9
3,510,635 + +0.6
3,511,699 - +0.7
3,511,714 + +1.0
3,511,714 + +0.2
3,511,714 + -0.2
3,511,793 + gph b3385 0.18 -0.8
3,511,795 + gph b3385 0.19 -0.6
3,511,820 + gph b3385 0.22 -0.5
3,511,825 - gph b3385 0.23 -0.2
3,511,851 + gph b3385 0.26 -0.8
3,511,851 + gph b3385 0.26 -1.1
3,511,856 - gph b3385 0.27 +1.1
3,511,877 + gph b3385 0.30 -1.4
3,511,877 + gph b3385 0.30 +0.3
3,511,887 + gph b3385 0.31 -0.6
3,511,897 - gph b3385 0.32 -0.6
3,511,925 - gph b3385 0.36 -0.4
3,511,950 - gph b3385 0.39 -1.3
3,511,950 - gph b3385 0.39 -1.4
3,511,965 + gph b3385 0.41 +0.3
3,511,965 + gph b3385 0.41 +0.1
3,511,965 + gph b3385 0.41 +0.1
3,511,973 - gph b3385 0.42 -0.3
3,511,975 - gph b3385 0.42 -1.2
3,511,975 - gph b3385 0.42 -0.5
3,511,985 - gph b3385 0.44 -0.6
3,512,007 + gph b3385 0.47 +0.2
3,512,011 - gph b3385 0.47 +0.5
3,512,020 - gph b3385 0.48 -0.1
3,512,021 - gph b3385 0.48 -1.2
3,512,026 - gph b3385 0.49 -1.2
3,512,030 + gph b3385 0.50 +0.4
3,512,038 - gph b3385 0.51 -1.5
3,512,038 - gph b3385 0.51 -3.1
3,512,038 - gph b3385 0.51 +0.6
3,512,104 + gph b3385 0.59 -0.0
3,512,109 + gph b3385 0.60 +0.1
3,512,132 + gph b3385 0.63 +0.1
3,512,140 - gph b3385 0.64 +0.1
3,512,140 - gph b3385 0.64 -0.6
3,512,140 - gph b3385 0.64 -0.5
3,512,166 + gph b3385 0.68 -0.4
3,512,166 + gph b3385 0.68 -0.0
3,512,172 - gph b3385 0.68 +0.5
3,512,177 + gph b3385 0.69 -1.2
3,512,216 - gph b3385 0.74 +0.3
3,512,217 + gph b3385 0.74 +2.0
3,512,218 + gph b3385 0.74 +0.2
3,512,226 - gph b3385 0.75 +0.1
3,512,226 - gph b3385 0.75 -0.1
3,512,235 + gph b3385 0.77 -0.4
3,512,246 + gph b3385 0.78 +0.6
3,512,295 + gph b3385 0.85 -0.0
3,512,295 + gph b3385 0.85 -0.4
3,512,313 - gph b3385 0.87 +0.2
3,512,313 - gph b3385 0.87 -0.4
3,512,345 + +0.2
3,512,345 + +0.1
3,512,352 + -0.7
3,512,358 - +0.5
3,512,360 - -1.2
3,512,360 - -0.6
3,512,368 - +0.4
3,512,425 - +0.2

Or see this region's nucleotide sequence