Experiment: D-Trehalose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sufS and sufD overlap by 4 nucleotides sufD and sufC overlap by 26 nucleotides sufC and ynhE are separated by 9 nucleotides
b1680: sufS - selenocysteine lyase (NCBI), at 1,757,327 to 1,758,547
sufS
b1681: sufD - component of SufBCD complex (NCBI), at 1,758,544 to 1,759,815
sufD
b1682: sufC - cysteine desulfurase ATPase component (NCBI), at 1,759,790 to 1,760,536
sufC
b1683: ynhE - orf, hypothetical protein (VIMSS), at 1,760,546 to 1,762,033
ynhE
Position (kb)
1758
1759
1760 Strain fitness (log2 ratio)
-1
0
1
2 at 1757.561 kb on - strand, within sufS at 1757.561 kb on - strand, within sufS at 1757.579 kb on + strand, within sufS at 1757.579 kb on + strand, within sufS at 1757.646 kb on + strand, within sufS at 1757.646 kb on + strand, within sufS at 1757.680 kb on - strand, within sufS at 1757.680 kb on - strand, within sufS at 1757.706 kb on + strand, within sufS at 1757.706 kb on + strand, within sufS at 1757.765 kb on - strand, within sufS at 1757.775 kb on - strand, within sufS at 1757.775 kb on - strand, within sufS at 1758.135 kb on - strand, within sufS at 1758.135 kb on - strand, within sufS at 1758.168 kb on + strand, within sufS at 1758.607 kb on + strand at 1758.670 kb on - strand at 1758.690 kb on + strand, within sufD at 1758.690 kb on + strand, within sufD at 1758.821 kb on - strand, within sufD at 1759.046 kb on - strand, within sufD at 1759.046 kb on - strand, within sufD at 1759.054 kb on + strand, within sufD at 1759.134 kb on + strand, within sufD at 1759.288 kb on + strand, within sufD at 1759.296 kb on - strand, within sufD at 1759.345 kb on - strand, within sufD at 1759.576 kb on - strand, within sufD at 1759.578 kb on - strand, within sufD at 1759.578 kb on - strand, within sufD at 1759.590 kb on - strand, within sufD at 1759.590 kb on - strand, within sufD at 1759.624 kb on + strand, within sufD at 1759.624 kb on + strand, within sufD at 1759.806 kb on - strand at 1759.806 kb on - strand at 1759.812 kb on + strand at 1759.812 kb on + strand at 1759.877 kb on + strand, within sufC at 1759.902 kb on - strand, within sufC at 1759.902 kb on - strand, within sufC at 1759.907 kb on - strand, within sufC at 1759.944 kb on + strand, within sufC at 1759.944 kb on + strand, within sufC at 1759.999 kb on + strand, within sufC at 1760.009 kb on - strand, within sufC at 1760.777 kb on - strand, within ynhE at 1760.788 kb on - strand, within ynhE
Per-strain Table
Position Strand Gene LocusTag Fraction D-Trehalose (C) remove 1,757,561 - sufS b1680 0.19 +0.4 1,757,561 - sufS b1680 0.19 +0.8 1,757,579 + sufS b1680 0.21 +1.5 1,757,579 + sufS b1680 0.21 +2.3 1,757,646 + sufS b1680 0.26 +1.3 1,757,646 + sufS b1680 0.26 +0.8 1,757,680 - sufS b1680 0.29 +0.1 1,757,680 - sufS b1680 0.29 -0.2 1,757,706 + sufS b1680 0.31 +0.2 1,757,706 + sufS b1680 0.31 +1.0 1,757,765 - sufS b1680 0.36 +0.7 1,757,775 - sufS b1680 0.37 -0.1 1,757,775 - sufS b1680 0.37 +0.0 1,758,135 - sufS b1680 0.66 +0.5 1,758,135 - sufS b1680 0.66 +0.2 1,758,168 + sufS b1680 0.69 +0.4 1,758,607 + -0.0 1,758,670 - -0.1 1,758,690 + sufD b1681 0.11 +0.3 1,758,690 + sufD b1681 0.11 -0.6 1,758,821 - sufD b1681 0.22 +0.6 1,759,046 - sufD b1681 0.39 -0.0 1,759,046 - sufD b1681 0.39 -0.3 1,759,054 + sufD b1681 0.40 +1.0 1,759,134 + sufD b1681 0.46 +0.4 1,759,288 + sufD b1681 0.58 +1.0 1,759,296 - sufD b1681 0.59 +0.3 1,759,345 - sufD b1681 0.63 +0.4 1,759,576 - sufD b1681 0.81 +0.8 1,759,578 - sufD b1681 0.81 +0.6 1,759,578 - sufD b1681 0.81 +0.3 1,759,590 - sufD b1681 0.82 +0.6 1,759,590 - sufD b1681 0.82 -0.2 1,759,624 + sufD b1681 0.85 +1.1 1,759,624 + sufD b1681 0.85 +0.1 1,759,806 - +0.4 1,759,806 - +0.8 1,759,812 + +0.2 1,759,812 + -0.3 1,759,877 + sufC b1682 0.12 -0.3 1,759,902 - sufC b1682 0.15 +0.1 1,759,902 - sufC b1682 0.15 +1.1 1,759,907 - sufC b1682 0.16 +0.6 1,759,944 + sufC b1682 0.21 +0.8 1,759,944 + sufC b1682 0.21 +0.5 1,759,999 + sufC b1682 0.28 +0.6 1,760,009 - sufC b1682 0.29 +0.4 1,760,777 - ynhE b1683 0.16 +0.5 1,760,788 - ynhE b1683 0.16 +0.6
Or see this region's nucleotide sequence