Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_18805 and IAI46_18810 are separated by 352 nucleotides IAI46_18810 and IAI46_18815 are separated by 60 nucleotides IAI46_18815 and IAI46_18820 are separated by 200 nucleotides IAI46_18820 and IAI46_18825 are separated by 29 nucleotides IAI46_18825 and IAI46_18830 are separated by 106 nucleotides
IAI46_18805: IAI46_18805 - YncE family protein, at 3,961,010 to 3,962,095
_18805
IAI46_18810: IAI46_18810 - TOBE domain-containing protein, at 3,962,448 to 3,962,876
_18810
IAI46_18815: IAI46_18815 - LysE family translocator, at 3,962,937 to 3,963,554
_18815
IAI46_18820: IAI46_18820 - Lrp/AsnC family transcriptional regulator, at 3,963,755 to 3,964,210
_18820
IAI46_18825: IAI46_18825 - DUF2767 family protein, at 3,964,240 to 3,964,446
_18825
IAI46_18830: IAI46_18830 - FAD-dependent oxidoreductase, at 3,964,553 to 3,965,761
_18830
Position (kb)
3962
3963
3964 Strain fitness (log2 ratio)
-2
-1
0
1 at 3962.114 kb on - strand at 3962.115 kb on + strand at 3962.115 kb on + strand at 3962.116 kb on - strand at 3962.116 kb on - strand at 3962.116 kb on - strand at 3962.185 kb on - strand at 3962.185 kb on - strand at 3962.367 kb on - strand at 3962.409 kb on + strand at 3962.409 kb on + strand at 3962.410 kb on - strand at 3962.509 kb on + strand, within IAI46_18810 at 3962.559 kb on - strand, within IAI46_18810 at 3962.569 kb on + strand, within IAI46_18810 at 3962.569 kb on + strand, within IAI46_18810 at 3962.639 kb on + strand, within IAI46_18810 at 3962.745 kb on - strand, within IAI46_18810 at 3962.875 kb on - strand at 3962.875 kb on - strand at 3962.944 kb on - strand at 3963.070 kb on + strand, within IAI46_18815 at 3963.187 kb on - strand, within IAI46_18815 at 3963.187 kb on - strand, within IAI46_18815 at 3963.353 kb on + strand, within IAI46_18815 at 3963.407 kb on + strand, within IAI46_18815 at 3963.430 kb on - strand, within IAI46_18815 at 3963.433 kb on - strand, within IAI46_18815 at 3963.478 kb on + strand, within IAI46_18815 at 3963.478 kb on + strand, within IAI46_18815 at 3963.479 kb on - strand, within IAI46_18815 at 3963.501 kb on - strand at 3963.501 kb on - strand at 3963.558 kb on + strand at 3963.558 kb on + strand at 3963.559 kb on - strand at 3963.725 kb on + strand at 3963.903 kb on - strand, within IAI46_18820 at 3963.938 kb on - strand, within IAI46_18820 at 3964.209 kb on - strand at 3964.239 kb on + strand at 3964.239 kb on + strand at 3964.240 kb on - strand at 3964.273 kb on + strand, within IAI46_18825 at 3964.273 kb on + strand, within IAI46_18825 at 3964.283 kb on + strand, within IAI46_18825 at 3964.283 kb on + strand, within IAI46_18825 at 3964.296 kb on - strand, within IAI46_18825 at 3964.365 kb on + strand, within IAI46_18825 at 3964.444 kb on + strand at 3964.444 kb on + strand at 3964.444 kb on + strand at 3964.444 kb on + strand at 3964.445 kb on - strand at 3964.469 kb on + strand at 3964.545 kb on + strand at 3964.546 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 3,962,114 - +0.7 3,962,115 + +0.9 3,962,115 + -0.3 3,962,116 - +0.4 3,962,116 - +0.2 3,962,116 - +1.3 3,962,185 - +1.2 3,962,185 - -0.0 3,962,367 - -0.2 3,962,409 + +0.1 3,962,409 + -0.6 3,962,410 - +0.1 3,962,509 + IAI46_18810 0.14 -1.3 3,962,559 - IAI46_18810 0.26 +1.1 3,962,569 + IAI46_18810 0.28 -1.1 3,962,569 + IAI46_18810 0.28 +0.6 3,962,639 + IAI46_18810 0.45 +0.7 3,962,745 - IAI46_18810 0.69 +0.7 3,962,875 - -0.8 3,962,875 - +0.1 3,962,944 - +0.8 3,963,070 + IAI46_18815 0.22 +0.3 3,963,187 - IAI46_18815 0.40 -0.5 3,963,187 - IAI46_18815 0.40 +0.4 3,963,353 + IAI46_18815 0.67 +0.1 3,963,407 + IAI46_18815 0.76 +0.1 3,963,430 - IAI46_18815 0.80 -0.2 3,963,433 - IAI46_18815 0.80 +0.2 3,963,478 + IAI46_18815 0.88 -0.0 3,963,478 + IAI46_18815 0.88 -0.8 3,963,479 - IAI46_18815 0.88 -0.3 3,963,501 - -0.3 3,963,501 - -0.4 3,963,558 + -1.5 3,963,558 + -1.0 3,963,559 - -0.4 3,963,725 + +0.6 3,963,903 - IAI46_18820 0.32 -0.1 3,963,938 - IAI46_18820 0.40 -1.5 3,964,209 - +0.5 3,964,239 + +1.1 3,964,239 + -0.0 3,964,240 - +0.6 3,964,273 + IAI46_18825 0.16 +0.7 3,964,273 + IAI46_18825 0.16 -0.5 3,964,283 + IAI46_18825 0.21 +0.1 3,964,283 + IAI46_18825 0.21 -0.1 3,964,296 - IAI46_18825 0.27 -1.5 3,964,365 + IAI46_18825 0.60 -0.1 3,964,444 + -1.2 3,964,444 + +0.7 3,964,444 + -0.3 3,964,444 + -0.4 3,964,445 - +0.2 3,964,469 + +1.1 3,964,545 + -1.9 3,964,546 - +0.3
Or see this region's nucleotide sequence