Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_11125 and IAI46_11130 are separated by 243 nucleotides IAI46_11130 and IAI46_11135 overlap by 4 nucleotides IAI46_11135 and IAI46_11140 are separated by 54 nucleotides
IAI46_11125: IAI46_11125 - AI-2E family transporter YdiK, at 2,351,236 to 2,352,336
_11125
IAI46_11130: IAI46_11130 - FAD-binding oxidoreductase, at 2,352,580 to 2,355,636
_11130
IAI46_11135: IAI46_11135 - 1,4-dihydroxy-2-naphthoyl-CoA hydrolase, at 2,355,633 to 2,356,049
_11135
IAI46_11140: IAI46_11140 - hypothetical protein, at 2,356,104 to 2,356,508
_11140
Position (kb)
2352
2353
2354
2355
2356 Strain fitness (log2 ratio)
-2
-1
0
1 at 2351.662 kb on + strand, within IAI46_11125 at 2351.802 kb on - strand, within IAI46_11125 at 2351.809 kb on + strand, within IAI46_11125 at 2351.810 kb on - strand, within IAI46_11125 at 2351.810 kb on - strand, within IAI46_11125 at 2351.840 kb on - strand, within IAI46_11125 at 2351.856 kb on + strand, within IAI46_11125 at 2351.856 kb on + strand, within IAI46_11125 at 2351.949 kb on + strand, within IAI46_11125 at 2351.950 kb on - strand, within IAI46_11125 at 2351.955 kb on - strand, within IAI46_11125 at 2351.963 kb on + strand, within IAI46_11125 at 2352.015 kb on + strand, within IAI46_11125 at 2352.033 kb on + strand, within IAI46_11125 at 2352.362 kb on - strand at 2352.362 kb on - strand at 2352.380 kb on - strand at 2352.380 kb on - strand at 2352.447 kb on + strand at 2352.447 kb on + strand at 2352.469 kb on - strand at 2352.476 kb on + strand at 2352.476 kb on + strand at 2352.477 kb on - strand at 2352.519 kb on + strand at 2352.717 kb on + strand at 2352.717 kb on + strand at 2352.717 kb on + strand at 2352.718 kb on - strand at 2352.718 kb on - strand at 2352.718 kb on - strand at 2352.721 kb on + strand at 2352.722 kb on - strand at 2353.110 kb on - strand, within IAI46_11130 at 2353.304 kb on - strand, within IAI46_11130 at 2353.414 kb on - strand, within IAI46_11130 at 2353.560 kb on + strand, within IAI46_11130 at 2353.774 kb on + strand, within IAI46_11130 at 2353.775 kb on - strand, within IAI46_11130 at 2353.882 kb on + strand, within IAI46_11130 at 2353.882 kb on + strand, within IAI46_11130 at 2353.882 kb on + strand, within IAI46_11130 at 2353.883 kb on - strand, within IAI46_11130 at 2354.053 kb on + strand, within IAI46_11130 at 2354.102 kb on - strand, within IAI46_11130 at 2354.131 kb on + strand, within IAI46_11130 at 2354.282 kb on + strand, within IAI46_11130 at 2354.286 kb on + strand, within IAI46_11130 at 2354.286 kb on + strand, within IAI46_11130 at 2354.287 kb on - strand, within IAI46_11130 at 2354.287 kb on - strand, within IAI46_11130 at 2354.314 kb on - strand, within IAI46_11130 at 2354.350 kb on - strand, within IAI46_11130 at 2354.539 kb on + strand, within IAI46_11130 at 2354.619 kb on + strand, within IAI46_11130 at 2354.689 kb on + strand, within IAI46_11130 at 2354.734 kb on + strand, within IAI46_11130 at 2354.734 kb on + strand, within IAI46_11130 at 2354.734 kb on + strand, within IAI46_11130 at 2354.735 kb on - strand, within IAI46_11130 at 2354.736 kb on + strand, within IAI46_11130 at 2354.737 kb on - strand, within IAI46_11130 at 2354.974 kb on + strand, within IAI46_11130 at 2354.975 kb on - strand, within IAI46_11130 at 2354.978 kb on - strand, within IAI46_11130 at 2355.006 kb on - strand, within IAI46_11130 at 2355.075 kb on + strand, within IAI46_11130 at 2355.075 kb on + strand, within IAI46_11130 at 2355.205 kb on + strand, within IAI46_11130 at 2355.207 kb on + strand, within IAI46_11130 at 2355.320 kb on - strand, within IAI46_11130 at 2355.422 kb on + strand at 2355.449 kb on - strand at 2355.497 kb on + strand at 2355.497 kb on + strand at 2355.497 kb on + strand at 2355.498 kb on - strand at 2355.498 kb on - strand at 2355.498 kb on - strand at 2355.498 kb on - strand at 2355.597 kb on - strand at 2355.692 kb on + strand, within IAI46_11135 at 2355.693 kb on - strand, within IAI46_11135 at 2355.693 kb on - strand, within IAI46_11135 at 2355.693 kb on - strand, within IAI46_11135 at 2355.738 kb on + strand, within IAI46_11135 at 2355.846 kb on + strand, within IAI46_11135 at 2355.874 kb on + strand, within IAI46_11135 at 2355.875 kb on - strand, within IAI46_11135 at 2356.105 kb on + strand at 2356.283 kb on + strand, within IAI46_11140 at 2356.309 kb on + strand, within IAI46_11140 at 2356.310 kb on - strand, within IAI46_11140 at 2356.310 kb on - strand, within IAI46_11140 at 2356.310 kb on - strand, within IAI46_11140 at 2356.460 kb on + strand, within IAI46_11140 at 2356.460 kb on + strand, within IAI46_11140 at 2356.634 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 2,351,662 + IAI46_11125 0.39 -0.6 2,351,802 - IAI46_11125 0.51 +0.3 2,351,809 + IAI46_11125 0.52 +0.2 2,351,810 - IAI46_11125 0.52 +0.0 2,351,810 - IAI46_11125 0.52 -1.6 2,351,840 - IAI46_11125 0.55 +0.6 2,351,856 + IAI46_11125 0.56 -0.9 2,351,856 + IAI46_11125 0.56 -0.4 2,351,949 + IAI46_11125 0.65 -0.8 2,351,950 - IAI46_11125 0.65 +0.4 2,351,955 - IAI46_11125 0.65 -0.6 2,351,963 + IAI46_11125 0.66 +1.0 2,352,015 + IAI46_11125 0.71 +0.0 2,352,033 + IAI46_11125 0.72 +1.2 2,352,362 - +0.2 2,352,362 - +0.4 2,352,380 - -1.0 2,352,380 - +1.1 2,352,447 + +1.0 2,352,447 + +1.0 2,352,469 - -1.4 2,352,476 + -0.2 2,352,476 + -0.4 2,352,477 - -1.6 2,352,519 + +0.1 2,352,717 + +0.2 2,352,717 + -0.4 2,352,717 + -0.5 2,352,718 - -1.3 2,352,718 - -1.6 2,352,718 - -0.4 2,352,721 + -0.7 2,352,722 - +0.3 2,353,110 - IAI46_11130 0.17 +1.4 2,353,304 - IAI46_11130 0.24 -1.7 2,353,414 - IAI46_11130 0.27 +1.0 2,353,560 + IAI46_11130 0.32 +0.4 2,353,774 + IAI46_11130 0.39 -1.6 2,353,775 - IAI46_11130 0.39 -1.1 2,353,882 + IAI46_11130 0.43 -1.5 2,353,882 + IAI46_11130 0.43 -0.2 2,353,882 + IAI46_11130 0.43 +0.9 2,353,883 - IAI46_11130 0.43 +1.1 2,354,053 + IAI46_11130 0.48 +1.0 2,354,102 - IAI46_11130 0.50 -1.1 2,354,131 + IAI46_11130 0.51 +0.2 2,354,282 + IAI46_11130 0.56 -0.6 2,354,286 + IAI46_11130 0.56 +0.1 2,354,286 + IAI46_11130 0.56 -2.0 2,354,287 - IAI46_11130 0.56 +0.3 2,354,287 - IAI46_11130 0.56 +1.0 2,354,314 - IAI46_11130 0.57 +1.2 2,354,350 - IAI46_11130 0.58 +0.7 2,354,539 + IAI46_11130 0.64 -0.6 2,354,619 + IAI46_11130 0.67 +0.4 2,354,689 + IAI46_11130 0.69 -1.2 2,354,734 + IAI46_11130 0.70 +0.2 2,354,734 + IAI46_11130 0.70 -0.8 2,354,734 + IAI46_11130 0.70 +0.0 2,354,735 - IAI46_11130 0.70 +0.6 2,354,736 + IAI46_11130 0.71 -0.4 2,354,737 - IAI46_11130 0.71 -0.7 2,354,974 + IAI46_11130 0.78 +0.8 2,354,975 - IAI46_11130 0.78 +0.1 2,354,978 - IAI46_11130 0.78 -0.6 2,355,006 - IAI46_11130 0.79 +0.2 2,355,075 + IAI46_11130 0.82 -1.2 2,355,075 + IAI46_11130 0.82 -0.6 2,355,205 + IAI46_11130 0.86 +0.0 2,355,207 + IAI46_11130 0.86 -1.1 2,355,320 - IAI46_11130 0.90 -0.6 2,355,422 + -0.8 2,355,449 - -0.8 2,355,497 + -2.0 2,355,497 + +0.3 2,355,497 + +0.4 2,355,498 - -0.1 2,355,498 - -0.6 2,355,498 - -1.0 2,355,498 - +0.6 2,355,597 - -0.7 2,355,692 + IAI46_11135 0.14 +0.6 2,355,693 - IAI46_11135 0.14 -1.7 2,355,693 - IAI46_11135 0.14 +0.0 2,355,693 - IAI46_11135 0.14 -0.4 2,355,738 + IAI46_11135 0.25 +0.4 2,355,846 + IAI46_11135 0.51 +1.0 2,355,874 + IAI46_11135 0.58 -0.4 2,355,875 - IAI46_11135 0.58 -0.3 2,356,105 + +1.0 2,356,283 + IAI46_11140 0.44 -0.3 2,356,309 + IAI46_11140 0.51 +0.9 2,356,310 - IAI46_11140 0.51 +1.2 2,356,310 - IAI46_11140 0.51 +1.1 2,356,310 - IAI46_11140 0.51 -0.0 2,356,460 + IAI46_11140 0.88 +0.7 2,356,460 + IAI46_11140 0.88 -0.6 2,356,634 - +0.9
Or see this region's nucleotide sequence