Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_10040 and IAI46_10045 are separated by 7 nucleotides IAI46_10045 and IAI46_10050 are separated by 219 nucleotides IAI46_10050 and IAI46_10055 are separated by 177 nucleotides IAI46_10055 and IAI46_10060 are separated by 12 nucleotides IAI46_10060 and IAI46_10065 are separated by 42 nucleotides IAI46_10065 and IAI46_10070 overlap by 4 nucleotides
IAI46_10040: IAI46_10040 - DUF2975 domain-containing protein, at 2,120,560 to 2,121,123
_10040
IAI46_10045: IAI46_10045 - helix-turn-helix transcriptional regulator, at 2,121,131 to 2,121,346
_10045
IAI46_10050: IAI46_10050 - hypothetical protein, at 2,121,566 to 2,121,790
_10050
IAI46_10055: IAI46_10055 - hypothetical protein, at 2,121,968 to 2,122,288
_10055
IAI46_10060: IAI46_10060 - hypothetical protein, at 2,122,301 to 2,122,624
_10060
IAI46_10065: IAI46_10065 - hypothetical protein, at 2,122,667 to 2,123,026
_10065
IAI46_10070: IAI46_10070 - LysR family transcriptional regulator, at 2,123,023 to 2,123,130
_10070
Position (kb)
2121
2122
2123 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2121.141 kb on + strand at 2121.144 kb on + strand at 2121.144 kb on + strand at 2121.277 kb on + strand, within IAI46_10045 at 2121.382 kb on + strand at 2121.382 kb on + strand at 2121.394 kb on - strand at 2121.420 kb on + strand at 2121.460 kb on + strand at 2121.526 kb on + strand at 2121.800 kb on + strand at 2121.897 kb on - strand at 2122.041 kb on - strand, within IAI46_10055 at 2122.171 kb on + strand, within IAI46_10055 at 2122.186 kb on + strand, within IAI46_10055 at 2122.274 kb on - strand at 2122.475 kb on + strand, within IAI46_10060 at 2122.479 kb on + strand, within IAI46_10060 at 2122.480 kb on - strand, within IAI46_10060 at 2122.563 kb on + strand, within IAI46_10060 at 2122.622 kb on + strand at 2122.648 kb on + strand at 2122.649 kb on - strand at 2122.739 kb on + strand, within IAI46_10065 at 2122.739 kb on + strand, within IAI46_10065 at 2122.739 kb on + strand, within IAI46_10065 at 2122.739 kb on + strand, within IAI46_10065 at 2122.803 kb on + strand, within IAI46_10065 at 2122.810 kb on + strand, within IAI46_10065 at 2122.810 kb on + strand, within IAI46_10065 at 2122.811 kb on - strand, within IAI46_10065 at 2122.815 kb on + strand, within IAI46_10065 at 2122.919 kb on + strand, within IAI46_10065 at 2123.025 kb on - strand at 2123.092 kb on + strand, within IAI46_10070 at 2123.093 kb on - strand, within IAI46_10070 at 2123.222 kb on + strand at 2123.223 kb on - strand at 2123.228 kb on + strand at 2123.236 kb on + strand at 2123.237 kb on - strand at 2123.239 kb on + strand at 2123.240 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 2,121,141 + +0.4 2,121,144 + +0.4 2,121,144 + -0.6 2,121,277 + IAI46_10045 0.68 -1.1 2,121,382 + +0.9 2,121,382 + -0.3 2,121,394 - -0.3 2,121,420 + -1.1 2,121,460 + +1.0 2,121,526 + -1.6 2,121,800 + +1.3 2,121,897 - +0.9 2,122,041 - IAI46_10055 0.23 +0.4 2,122,171 + IAI46_10055 0.63 +0.4 2,122,186 + IAI46_10055 0.68 +0.1 2,122,274 - +1.5 2,122,475 + IAI46_10060 0.54 -0.1 2,122,479 + IAI46_10060 0.55 +1.3 2,122,480 - IAI46_10060 0.55 -0.1 2,122,563 + IAI46_10060 0.81 +0.3 2,122,622 + -2.1 2,122,648 + -1.1 2,122,649 - +0.3 2,122,739 + IAI46_10065 0.20 +1.3 2,122,739 + IAI46_10065 0.20 -0.5 2,122,739 + IAI46_10065 0.20 +0.3 2,122,739 + IAI46_10065 0.20 -0.3 2,122,803 + IAI46_10065 0.38 -0.1 2,122,810 + IAI46_10065 0.40 -0.1 2,122,810 + IAI46_10065 0.40 +0.9 2,122,811 - IAI46_10065 0.40 -0.8 2,122,815 + IAI46_10065 0.41 -0.5 2,122,919 + IAI46_10065 0.70 +0.3 2,123,025 - +1.1 2,123,092 + IAI46_10070 0.64 -0.6 2,123,093 - IAI46_10070 0.65 +0.3 2,123,222 + -0.1 2,123,223 - -0.3 2,123,228 + +1.4 2,123,236 + +0.3 2,123,237 - +0.5 2,123,239 + -2.8 2,123,240 - -1.0
Or see this region's nucleotide sequence