Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_03020 and IAI46_03025 are separated by 212 nucleotides IAI46_03025 and IAI46_03030 are separated by 82 nucleotides
IAI46_03020: IAI46_03020 - tRNA/rRNA methyltransferase, at 643,943 to 644,629
_03020
IAI46_03025: IAI46_03025 - thr operon leader peptide, at 644,842 to 644,910
_03025
IAI46_03030: IAI46_03030 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 644,993 to 647,452
_03030
Position (kb)
644
645 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 643.846 kb on + strand at 643.864 kb on + strand at 643.886 kb on + strand at 643.926 kb on + strand at 643.938 kb on + strand at 643.954 kb on + strand at 643.954 kb on + strand at 643.955 kb on - strand at 643.963 kb on - strand at 644.027 kb on - strand, within IAI46_03020 at 644.102 kb on - strand, within IAI46_03020 at 644.154 kb on + strand, within IAI46_03020 at 644.201 kb on + strand, within IAI46_03020 at 644.202 kb on - strand, within IAI46_03020 at 644.204 kb on + strand, within IAI46_03020 at 644.204 kb on + strand, within IAI46_03020 at 644.204 kb on + strand, within IAI46_03020 at 644.204 kb on + strand, within IAI46_03020 at 644.205 kb on - strand, within IAI46_03020 at 644.209 kb on + strand, within IAI46_03020 at 644.341 kb on - strand, within IAI46_03020 at 644.341 kb on - strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.344 kb on + strand, within IAI46_03020 at 644.345 kb on - strand, within IAI46_03020 at 644.345 kb on - strand, within IAI46_03020 at 644.345 kb on - strand, within IAI46_03020 at 644.345 kb on - strand, within IAI46_03020 at 644.345 kb on - strand, within IAI46_03020 at 644.345 kb on - strand, within IAI46_03020 at 644.393 kb on + strand, within IAI46_03020 at 644.393 kb on + strand, within IAI46_03020 at 644.394 kb on - strand, within IAI46_03020 at 644.394 kb on - strand, within IAI46_03020 at 644.394 kb on - strand, within IAI46_03020 at 644.394 kb on - strand, within IAI46_03020 at 644.394 kb on - strand, within IAI46_03020 at 644.399 kb on + strand, within IAI46_03020 at 644.400 kb on - strand, within IAI46_03020 at 644.472 kb on + strand, within IAI46_03020 at 644.472 kb on + strand, within IAI46_03020 at 644.473 kb on - strand, within IAI46_03020 at 644.473 kb on - strand, within IAI46_03020 at 644.590 kb on + strand at 644.590 kb on + strand at 644.636 kb on + strand at 644.636 kb on + strand at 644.646 kb on + strand at 644.749 kb on + strand at 644.749 kb on + strand at 644.750 kb on - strand at 644.750 kb on - strand at 644.753 kb on + strand at 644.754 kb on - strand at 644.804 kb on + strand at 644.804 kb on + strand at 644.818 kb on + strand at 644.819 kb on - strand at 644.819 kb on - strand at 644.823 kb on - strand at 644.823 kb on - strand at 644.823 kb on - strand at 644.872 kb on - strand, within IAI46_03025 at 644.872 kb on - strand, within IAI46_03025 at 644.874 kb on + strand, within IAI46_03025 at 644.874 kb on + strand, within IAI46_03025 at 644.945 kb on + strand at 644.945 kb on + strand at 644.946 kb on - strand at 644.946 kb on - strand at 645.071 kb on - strand at 645.071 kb on - strand at 645.181 kb on + strand at 645.181 kb on + strand at 645.182 kb on - strand at 645.358 kb on + strand, within IAI46_03030 at 645.358 kb on + strand, within IAI46_03030 at 645.454 kb on - strand, within IAI46_03030 at 645.486 kb on + strand, within IAI46_03030 at 645.487 kb on - strand, within IAI46_03030 at 645.490 kb on + strand, within IAI46_03030 at 645.617 kb on + strand, within IAI46_03030 at 645.689 kb on + strand, within IAI46_03030 at 645.691 kb on + strand, within IAI46_03030 at 645.692 kb on - strand, within IAI46_03030 at 645.692 kb on - strand, within IAI46_03030 at 645.767 kb on + strand, within IAI46_03030 at 645.902 kb on - strand, within IAI46_03030
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 643,846 + +0.7 643,864 + -0.3 643,886 + +0.7 643,926 + +0.4 643,938 + +0.0 643,954 + -0.1 643,954 + +1.2 643,955 - +0.5 643,963 - -0.2 644,027 - IAI46_03020 0.12 -0.7 644,102 - IAI46_03020 0.23 +0.3 644,154 + IAI46_03020 0.31 +0.0 644,201 + IAI46_03020 0.38 -0.6 644,202 - IAI46_03020 0.38 +0.0 644,204 + IAI46_03020 0.38 -0.8 644,204 + IAI46_03020 0.38 -0.1 644,204 + IAI46_03020 0.38 +0.6 644,204 + IAI46_03020 0.38 +0.7 644,205 - IAI46_03020 0.38 +1.1 644,209 + IAI46_03020 0.39 -0.5 644,341 - IAI46_03020 0.58 +0.1 644,341 - IAI46_03020 0.58 -1.7 644,344 + IAI46_03020 0.58 +0.0 644,344 + IAI46_03020 0.58 -1.6 644,344 + IAI46_03020 0.58 -0.8 644,344 + IAI46_03020 0.58 +0.3 644,344 + IAI46_03020 0.58 +0.1 644,344 + IAI46_03020 0.58 -0.0 644,344 + IAI46_03020 0.58 +2.5 644,345 - IAI46_03020 0.59 -0.1 644,345 - IAI46_03020 0.59 -0.3 644,345 - IAI46_03020 0.59 +0.6 644,345 - IAI46_03020 0.59 +0.1 644,345 - IAI46_03020 0.59 +0.3 644,345 - IAI46_03020 0.59 +0.1 644,393 + IAI46_03020 0.66 +0.3 644,393 + IAI46_03020 0.66 -0.5 644,394 - IAI46_03020 0.66 -1.1 644,394 - IAI46_03020 0.66 +0.8 644,394 - IAI46_03020 0.66 -0.3 644,394 - IAI46_03020 0.66 -0.5 644,394 - IAI46_03020 0.66 +0.9 644,399 + IAI46_03020 0.66 +0.2 644,400 - IAI46_03020 0.67 +0.6 644,472 + IAI46_03020 0.77 +0.1 644,472 + IAI46_03020 0.77 +0.7 644,473 - IAI46_03020 0.77 -0.6 644,473 - IAI46_03020 0.77 -1.1 644,590 + +0.8 644,590 + +1.0 644,636 + -0.7 644,636 + -1.0 644,646 + -0.3 644,749 + +0.2 644,749 + +0.5 644,750 - +0.7 644,750 - +0.2 644,753 + +0.0 644,754 - -0.9 644,804 + +0.2 644,804 + +0.4 644,818 + +0.7 644,819 - -0.5 644,819 - -0.7 644,823 - -0.1 644,823 - +0.7 644,823 - -2.0 644,872 - IAI46_03025 0.43 -1.3 644,872 - IAI46_03025 0.43 -1.2 644,874 + IAI46_03025 0.46 +0.3 644,874 + IAI46_03025 0.46 -0.1 644,945 + +0.5 644,945 + +0.4 644,946 - +0.9 644,946 - -1.9 645,071 - -1.4 645,071 - -1.0 645,181 + -1.5 645,181 + -1.9 645,182 - -1.1 645,358 + IAI46_03030 0.15 +0.2 645,358 + IAI46_03030 0.15 -1.1 645,454 - IAI46_03030 0.19 -0.7 645,486 + IAI46_03030 0.20 -0.1 645,487 - IAI46_03030 0.20 -0.5 645,490 + IAI46_03030 0.20 -0.2 645,617 + IAI46_03030 0.25 +0.7 645,689 + IAI46_03030 0.28 -1.4 645,691 + IAI46_03030 0.28 -2.1 645,692 - IAI46_03030 0.28 -1.2 645,692 - IAI46_03030 0.28 -1.1 645,767 + IAI46_03030 0.31 -0.8 645,902 - IAI46_03030 0.37 -0.6
Or see this region's nucleotide sequence