Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4326 and EX28DRAFT_4327 are separated by 193 nucleotides EX28DRAFT_4327 and EX28DRAFT_4328 are separated by 22 nucleotides
EX28DRAFT_4326: EX28DRAFT_4326 - methionine synthase (B12-dependent) (EC 2.1.1.13), at 130,048 to 133,731
_4326
EX28DRAFT_4327: EX28DRAFT_4327 - transcriptional regulator, IclR family, at 133,925 to 134,755
_4327
EX28DRAFT_4328: EX28DRAFT_4328 - Isocitrate dehydrogenase kinase/phosphatase, at 134,778 to 136,529
_4328
Position (kb) 133
134
135 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 132.930 kb on - strand, within EX28DRAFT_4326 at 132.958 kb on + strand, within EX28DRAFT_4326 at 132.958 kb on + strand, within EX28DRAFT_4326 at 132.959 kb on - strand, within EX28DRAFT_4326 at 132.959 kb on - strand, within EX28DRAFT_4326 at 132.959 kb on - strand, within EX28DRAFT_4326 at 132.963 kb on - strand, within EX28DRAFT_4326 at 133.035 kb on + strand, within EX28DRAFT_4326 at 133.037 kb on + strand, within EX28DRAFT_4326 at 133.037 kb on + strand, within EX28DRAFT_4326 at 133.038 kb on - strand, within EX28DRAFT_4326 at 133.272 kb on + strand, within EX28DRAFT_4326 at 133.273 kb on - strand, within EX28DRAFT_4326 at 133.480 kb on - strand at 133.540 kb on - strand at 133.540 kb on - strand at 133.717 kb on + strand at 133.718 kb on - strand at 133.718 kb on - strand at 134.365 kb on + strand, within EX28DRAFT_4327 at 134.366 kb on - strand, within EX28DRAFT_4327 at 134.486 kb on + strand, within EX28DRAFT_4327 at 134.487 kb on - strand, within EX28DRAFT_4327 at 135.060 kb on + strand, within EX28DRAFT_4328 at 135.060 kb on + strand, within EX28DRAFT_4328 at 135.060 kb on + strand, within EX28DRAFT_4328 at 135.061 kb on - strand, within EX28DRAFT_4328 at 135.061 kb on - strand, within EX28DRAFT_4328 at 135.089 kb on - strand, within EX28DRAFT_4328 at 135.110 kb on - strand, within EX28DRAFT_4328 at 135.174 kb on - strand, within EX28DRAFT_4328 at 135.208 kb on + strand, within EX28DRAFT_4328 at 135.516 kb on - strand, within EX28DRAFT_4328 at 135.524 kb on + strand, within EX28DRAFT_4328 at 135.524 kb on + strand, within EX28DRAFT_4328 at 135.552 kb on - strand, within EX28DRAFT_4328 at 135.570 kb on - strand, within EX28DRAFT_4328 at 135.570 kb on - strand, within EX28DRAFT_4328 at 135.619 kb on + strand, within EX28DRAFT_4328 at 135.619 kb on + strand, within EX28DRAFT_4328 at 135.620 kb on - strand, within EX28DRAFT_4328 at 135.678 kb on - strand, within EX28DRAFT_4328 at 135.678 kb on - strand, within EX28DRAFT_4328 at 135.678 kb on - strand, within EX28DRAFT_4328 at 135.679 kb on + strand, within EX28DRAFT_4328 at 135.679 kb on + strand, within EX28DRAFT_4328 at 135.679 kb on + strand, within EX28DRAFT_4328 at 135.680 kb on - strand, within EX28DRAFT_4328 at 135.680 kb on - strand, within EX28DRAFT_4328 at 135.680 kb on - strand, within EX28DRAFT_4328 at 135.680 kb on - strand, within EX28DRAFT_4328
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 132,930 - EX28DRAFT_4326 0.78 +0.2 132,958 + EX28DRAFT_4326 0.79 +0.3 132,958 + EX28DRAFT_4326 0.79 +0.5 132,959 - EX28DRAFT_4326 0.79 +0.5 132,959 - EX28DRAFT_4326 0.79 +0.5 132,959 - EX28DRAFT_4326 0.79 -0.3 132,963 - EX28DRAFT_4326 0.79 +2.9 133,035 + EX28DRAFT_4326 0.81 -0.3 133,037 + EX28DRAFT_4326 0.81 +0.3 133,037 + EX28DRAFT_4326 0.81 +0.4 133,038 - EX28DRAFT_4326 0.81 -0.6 133,272 + EX28DRAFT_4326 0.88 -0.4 133,273 - EX28DRAFT_4326 0.88 +0.9 133,480 - +0.5 133,540 - +0.3 133,540 - +0.3 133,717 + +0.2 133,718 - -0.2 133,718 - +0.9 134,365 + EX28DRAFT_4327 0.53 -0.6 134,366 - EX28DRAFT_4327 0.53 -0.1 134,486 + EX28DRAFT_4327 0.68 -1.3 134,487 - EX28DRAFT_4327 0.68 -0.1 135,060 + EX28DRAFT_4328 0.16 -1.3 135,060 + EX28DRAFT_4328 0.16 +0.6 135,060 + EX28DRAFT_4328 0.16 +0.2 135,061 - EX28DRAFT_4328 0.16 +1.0 135,061 - EX28DRAFT_4328 0.16 -1.9 135,089 - EX28DRAFT_4328 0.18 +0.4 135,110 - EX28DRAFT_4328 0.19 -0.1 135,174 - EX28DRAFT_4328 0.23 +0.0 135,208 + EX28DRAFT_4328 0.25 -0.3 135,516 - EX28DRAFT_4328 0.42 -0.9 135,524 + EX28DRAFT_4328 0.43 -1.1 135,524 + EX28DRAFT_4328 0.43 +0.2 135,552 - EX28DRAFT_4328 0.44 +0.1 135,570 - EX28DRAFT_4328 0.45 -1.9 135,570 - EX28DRAFT_4328 0.45 -0.2 135,619 + EX28DRAFT_4328 0.48 +0.3 135,619 + EX28DRAFT_4328 0.48 -0.3 135,620 - EX28DRAFT_4328 0.48 -0.7 135,678 - EX28DRAFT_4328 0.51 -0.0 135,678 - EX28DRAFT_4328 0.51 -0.1 135,678 - EX28DRAFT_4328 0.51 -1.4 135,679 + EX28DRAFT_4328 0.51 +0.3 135,679 + EX28DRAFT_4328 0.51 -2.3 135,679 + EX28DRAFT_4328 0.51 +1.2 135,680 - EX28DRAFT_4328 0.51 -0.1 135,680 - EX28DRAFT_4328 0.51 -0.4 135,680 - EX28DRAFT_4328 0.51 -0.7 135,680 - EX28DRAFT_4328 0.51 +0.0
Or see this region's nucleotide sequence