Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3954 and EX28DRAFT_3955 are separated by 312 nucleotides EX28DRAFT_3955 and EX28DRAFT_3956 are separated by 21 nucleotides EX28DRAFT_3956 and EX28DRAFT_3957 overlap by 8 nucleotides
EX28DRAFT_3954: EX28DRAFT_3954 - acetolactate synthase, large subunit (EC 2.2.1.6), at 164,026 to 165,714
_3954
EX28DRAFT_3955: EX28DRAFT_3955 - type VI secretion system effector, Hcp1 family, at 166,027 to 166,506
_3955
EX28DRAFT_3956: EX28DRAFT_3956 - hypothetical protein, at 166,528 to 166,710
_3956
EX28DRAFT_3957: EX28DRAFT_3957 - hypothetical protein, at 166,703 to 167,416
_3957
Position (kb)
166
167 Strain fitness (log2 ratio)
-1
0
1
2 at 165.587 kb on + strand at 165.588 kb on - strand at 165.588 kb on - strand at 165.588 kb on - strand at 165.953 kb on + strand at 165.956 kb on - strand at 166.059 kb on + strand at 166.061 kb on + strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.062 kb on - strand at 166.152 kb on - strand, within EX28DRAFT_3955 at 166.201 kb on - strand, within EX28DRAFT_3955 at 166.342 kb on + strand, within EX28DRAFT_3955 at 166.342 kb on + strand, within EX28DRAFT_3955 at 166.395 kb on + strand, within EX28DRAFT_3955 at 166.395 kb on + strand, within EX28DRAFT_3955 at 166.600 kb on + strand, within EX28DRAFT_3956 at 166.603 kb on + strand, within EX28DRAFT_3956 at 166.604 kb on - strand, within EX28DRAFT_3956 at 166.725 kb on - strand at 166.836 kb on + strand, within EX28DRAFT_3957 at 166.851 kb on + strand, within EX28DRAFT_3957 at 166.936 kb on + strand, within EX28DRAFT_3957 at 166.937 kb on - strand, within EX28DRAFT_3957 at 166.940 kb on + strand, within EX28DRAFT_3957 at 166.940 kb on + strand, within EX28DRAFT_3957 at 166.941 kb on - strand, within EX28DRAFT_3957 at 166.980 kb on - strand, within EX28DRAFT_3957 at 166.980 kb on - strand, within EX28DRAFT_3957 at 167.038 kb on + strand, within EX28DRAFT_3957 at 167.038 kb on + strand, within EX28DRAFT_3957 at 167.039 kb on - strand, within EX28DRAFT_3957 at 167.048 kb on + strand, within EX28DRAFT_3957 at 167.049 kb on - strand, within EX28DRAFT_3957 at 167.086 kb on + strand, within EX28DRAFT_3957 at 167.087 kb on - strand, within EX28DRAFT_3957 at 167.148 kb on + strand, within EX28DRAFT_3957 at 167.166 kb on - strand, within EX28DRAFT_3957 at 167.673 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 165,587 + -0.0 165,588 - +1.8 165,588 - +0.3 165,588 - -0.5 165,953 + -0.5 165,956 - -0.1 166,059 + +1.2 166,061 + +0.8 166,062 - -0.5 166,062 - -1.1 166,062 - +0.1 166,062 - -0.2 166,062 - -0.3 166,062 - -1.0 166,062 - +1.0 166,062 - -0.5 166,152 - EX28DRAFT_3955 0.26 -0.9 166,201 - EX28DRAFT_3955 0.36 +0.5 166,342 + EX28DRAFT_3955 0.66 -0.9 166,342 + EX28DRAFT_3955 0.66 -0.3 166,395 + EX28DRAFT_3955 0.77 -0.3 166,395 + EX28DRAFT_3955 0.77 +0.3 166,600 + EX28DRAFT_3956 0.39 -1.2 166,603 + EX28DRAFT_3956 0.41 +0.0 166,604 - EX28DRAFT_3956 0.42 -1.3 166,725 - +0.2 166,836 + EX28DRAFT_3957 0.19 +0.3 166,851 + EX28DRAFT_3957 0.21 -0.5 166,936 + EX28DRAFT_3957 0.33 -0.3 166,937 - EX28DRAFT_3957 0.33 +0.0 166,940 + EX28DRAFT_3957 0.33 -0.9 166,940 + EX28DRAFT_3957 0.33 +0.4 166,941 - EX28DRAFT_3957 0.33 -0.2 166,980 - EX28DRAFT_3957 0.39 +0.2 166,980 - EX28DRAFT_3957 0.39 +0.2 167,038 + EX28DRAFT_3957 0.47 -1.0 167,038 + EX28DRAFT_3957 0.47 -0.1 167,039 - EX28DRAFT_3957 0.47 -0.3 167,048 + EX28DRAFT_3957 0.48 +0.6 167,049 - EX28DRAFT_3957 0.48 +0.2 167,086 + EX28DRAFT_3957 0.54 -0.2 167,087 - EX28DRAFT_3957 0.54 -0.2 167,148 + EX28DRAFT_3957 0.62 +0.4 167,166 - EX28DRAFT_3957 0.65 -0.2 167,673 + +0.1
Or see this region's nucleotide sequence