Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3750 and EX28DRAFT_3751 are separated by 146 nucleotides EX28DRAFT_3751 and EX28DRAFT_3752 are separated by 92 nucleotides EX28DRAFT_3752 and EX28DRAFT_3753 are separated by 111 nucleotides
EX28DRAFT_3750: EX28DRAFT_3750 - periplasmic serine protease, Do/DeqQ family, at 429,558 to 430,994
_3750
EX28DRAFT_3751: EX28DRAFT_3751 - Sugar diacid utilization regulator, at 431,141 to 432,298
_3751
EX28DRAFT_3752: EX28DRAFT_3752 - Protein of unknown function (DUF3461), at 432,391 to 432,777
_3752
EX28DRAFT_3753: EX28DRAFT_3753 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 432,889 to 433,713
_3753
Position (kb) 431
432
433 Strain fitness (log2 ratio) -2
-1
0
1 at 430.380 kb on - strand, within EX28DRAFT_3750 at 430.653 kb on - strand, within EX28DRAFT_3750 at 430.682 kb on + strand, within EX28DRAFT_3750 at 431.110 kb on + strand at 431.138 kb on + strand at 431.138 kb on + strand at 431.139 kb on - strand at 431.180 kb on - strand at 431.181 kb on - strand at 431.209 kb on + strand at 431.209 kb on + strand at 431.216 kb on - strand at 431.223 kb on + strand at 431.468 kb on - strand, within EX28DRAFT_3751 at 431.558 kb on - strand, within EX28DRAFT_3751 at 431.575 kb on + strand, within EX28DRAFT_3751 at 431.575 kb on + strand, within EX28DRAFT_3751 at 431.576 kb on - strand, within EX28DRAFT_3751 at 431.897 kb on + strand, within EX28DRAFT_3751 at 431.897 kb on + strand, within EX28DRAFT_3751 at 431.897 kb on + strand, within EX28DRAFT_3751 at 431.897 kb on + strand, within EX28DRAFT_3751 at 431.898 kb on - strand, within EX28DRAFT_3751 at 431.898 kb on - strand, within EX28DRAFT_3751 at 431.898 kb on - strand, within EX28DRAFT_3751 at 431.990 kb on + strand, within EX28DRAFT_3751 at 431.991 kb on - strand, within EX28DRAFT_3751 at 431.991 kb on - strand, within EX28DRAFT_3751 at 431.991 kb on - strand, within EX28DRAFT_3751 at 431.991 kb on - strand, within EX28DRAFT_3751 at 432.198 kb on - strand at 432.266 kb on + strand at 432.296 kb on + strand at 432.297 kb on - strand at 432.297 kb on - strand at 432.297 kb on - strand at 432.446 kb on - strand, within EX28DRAFT_3752 at 432.546 kb on - strand, within EX28DRAFT_3752 at 432.546 kb on - strand, within EX28DRAFT_3752 at 432.592 kb on + strand, within EX28DRAFT_3752 at 432.615 kb on + strand, within EX28DRAFT_3752 at 432.629 kb on + strand, within EX28DRAFT_3752 at 432.748 kb on - strand at 432.773 kb on + strand at 432.849 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 430,380 - EX28DRAFT_3750 0.57 -0.4 430,653 - EX28DRAFT_3750 0.76 +0.7 430,682 + EX28DRAFT_3750 0.78 -0.1 431,110 + +0.8 431,138 + +0.1 431,138 + -1.7 431,139 - -0.0 431,180 - +0.0 431,181 - +0.5 431,209 + +0.1 431,209 + +0.2 431,216 - +0.0 431,223 + +0.1 431,468 - EX28DRAFT_3751 0.28 -0.6 431,558 - EX28DRAFT_3751 0.36 +0.6 431,575 + EX28DRAFT_3751 0.37 +0.3 431,575 + EX28DRAFT_3751 0.37 +0.6 431,576 - EX28DRAFT_3751 0.38 +0.0 431,897 + EX28DRAFT_3751 0.65 +0.8 431,897 + EX28DRAFT_3751 0.65 -0.1 431,897 + EX28DRAFT_3751 0.65 +0.3 431,897 + EX28DRAFT_3751 0.65 -0.1 431,898 - EX28DRAFT_3751 0.65 -1.9 431,898 - EX28DRAFT_3751 0.65 -0.3 431,898 - EX28DRAFT_3751 0.65 -1.3 431,990 + EX28DRAFT_3751 0.73 +0.8 431,991 - EX28DRAFT_3751 0.73 -0.1 431,991 - EX28DRAFT_3751 0.73 +0.3 431,991 - EX28DRAFT_3751 0.73 -0.3 431,991 - EX28DRAFT_3751 0.73 +1.0 432,198 - -1.4 432,266 + +0.7 432,296 + -2.0 432,297 - -0.3 432,297 - +0.2 432,297 - +0.8 432,446 - EX28DRAFT_3752 0.14 +0.1 432,546 - EX28DRAFT_3752 0.40 -0.6 432,546 - EX28DRAFT_3752 0.40 -0.7 432,592 + EX28DRAFT_3752 0.52 +0.0 432,615 + EX28DRAFT_3752 0.58 -0.4 432,629 + EX28DRAFT_3752 0.61 -0.9 432,748 - -0.5 432,773 + +0.5 432,849 + -0.5
Or see this region's nucleotide sequence