Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_3547

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_3546 and EX28DRAFT_3547 overlap by 4 nucleotidesEX28DRAFT_3547 and EX28DRAFT_3548 are separated by 270 nucleotidesEX28DRAFT_3548 and EX28DRAFT_3549 are separated by 83 nucleotides EX28DRAFT_3546: EX28DRAFT_3546 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 209,532 to 210,998 _3546 EX28DRAFT_3547: EX28DRAFT_3547 - 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, C-terminal subunit/4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase, N-terminal subunit, at 210,995 to 212,272 _3547 EX28DRAFT_3548: EX28DRAFT_3548 - homoprotocatechuate degradation operon regulator, HpaR, at 212,543 to 212,983 _3548 EX28DRAFT_3549: EX28DRAFT_3549 - methyl-accepting chemotaxis sensory transducer with TarH sensor, at 213,067 to 214,731 _3549 Position (kb) 210 211 212 213Strain fitness (log2 ratio) -2 -1 0 1at 210.277 kb on + strand, within EX28DRAFT_3546at 210.277 kb on + strand, within EX28DRAFT_3546at 210.277 kb on + strand, within EX28DRAFT_3546at 210.278 kb on - strand, within EX28DRAFT_3546at 210.604 kb on + strand, within EX28DRAFT_3546at 210.604 kb on + strand, within EX28DRAFT_3546at 210.604 kb on + strand, within EX28DRAFT_3546at 210.604 kb on + strand, within EX28DRAFT_3546at 210.604 kb on + strand, within EX28DRAFT_3546at 210.604 kb on + strand, within EX28DRAFT_3546at 210.605 kb on - strand, within EX28DRAFT_3546at 210.605 kb on - strand, within EX28DRAFT_3546at 210.912 kb on + strandat 211.139 kb on - strand, within EX28DRAFT_3547at 211.152 kb on + strand, within EX28DRAFT_3547at 211.336 kb on - strand, within EX28DRAFT_3547at 211.393 kb on - strand, within EX28DRAFT_3547at 211.470 kb on + strand, within EX28DRAFT_3547at 211.470 kb on + strand, within EX28DRAFT_3547at 211.470 kb on + strand, within EX28DRAFT_3547at 211.471 kb on - strand, within EX28DRAFT_3547at 211.471 kb on - strand, within EX28DRAFT_3547at 211.471 kb on - strand, within EX28DRAFT_3547at 211.471 kb on - strand, within EX28DRAFT_3547at 211.535 kb on - strand, within EX28DRAFT_3547at 211.535 kb on - strand, within EX28DRAFT_3547at 211.538 kb on + strand, within EX28DRAFT_3547at 211.590 kb on + strand, within EX28DRAFT_3547at 211.610 kb on + strand, within EX28DRAFT_3547at 211.610 kb on + strand, within EX28DRAFT_3547at 212.159 kb on - strandat 212.190 kb on + strandat 212.190 kb on + strandat 212.278 kb on + strandat 212.278 kb on + strandat 212.278 kb on + strandat 212.278 kb on + strandat 212.279 kb on - strandat 212.279 kb on - strandat 212.804 kb on + strand, within EX28DRAFT_3548at 212.804 kb on + strand, within EX28DRAFT_3548at 212.805 kb on - strand, within EX28DRAFT_3548at 212.838 kb on - strand, within EX28DRAFT_3548at 212.938 kb on + strand, within EX28DRAFT_3548at 212.939 kb on - strand, within EX28DRAFT_3548at 213.071 kb on + strandat 213.071 kb on + strandat 213.071 kb on + strandat 213.116 kb on + strandat 213.128 kb on + strandat 213.129 kb on - strandat 213.261 kb on + strand, within EX28DRAFT_3549at 213.261 kb on + strand, within EX28DRAFT_3549

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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210,277 + EX28DRAFT_3546 0.51 +1.7
210,277 + EX28DRAFT_3546 0.51 -0.6
210,277 + EX28DRAFT_3546 0.51 -0.5
210,278 - EX28DRAFT_3546 0.51 +0.1
210,604 + EX28DRAFT_3546 0.73 -0.1
210,604 + EX28DRAFT_3546 0.73 -0.4
210,604 + EX28DRAFT_3546 0.73 +0.0
210,604 + EX28DRAFT_3546 0.73 +0.5
210,604 + EX28DRAFT_3546 0.73 -0.3
210,604 + EX28DRAFT_3546 0.73 +0.3
210,605 - EX28DRAFT_3546 0.73 +0.3
210,605 - EX28DRAFT_3546 0.73 +0.1
210,912 + +0.2
211,139 - EX28DRAFT_3547 0.11 -0.1
211,152 + EX28DRAFT_3547 0.12 -0.5
211,336 - EX28DRAFT_3547 0.27 -0.5
211,393 - EX28DRAFT_3547 0.31 +0.6
211,470 + EX28DRAFT_3547 0.37 +0.1
211,470 + EX28DRAFT_3547 0.37 +0.2
211,470 + EX28DRAFT_3547 0.37 +0.1
211,471 - EX28DRAFT_3547 0.37 -0.3
211,471 - EX28DRAFT_3547 0.37 -0.1
211,471 - EX28DRAFT_3547 0.37 +0.1
211,471 - EX28DRAFT_3547 0.37 -0.4
211,535 - EX28DRAFT_3547 0.42 -0.4
211,535 - EX28DRAFT_3547 0.42 -0.3
211,538 + EX28DRAFT_3547 0.42 -1.0
211,590 + EX28DRAFT_3547 0.47 -0.1
211,610 + EX28DRAFT_3547 0.48 -0.2
211,610 + EX28DRAFT_3547 0.48 -1.9
212,159 - -0.1
212,190 + -0.9
212,190 + +0.2
212,278 + +0.3
212,278 + +0.3
212,278 + +0.6
212,278 + -0.2
212,279 - +0.4
212,279 - +0.7
212,804 + EX28DRAFT_3548 0.59 -0.6
212,804 + EX28DRAFT_3548 0.59 +0.0
212,805 - EX28DRAFT_3548 0.59 +0.3
212,838 - EX28DRAFT_3548 0.67 -0.1
212,938 + EX28DRAFT_3548 0.90 +0.1
212,939 - EX28DRAFT_3548 0.90 +0.3
213,071 + -0.2
213,071 + -1.2
213,071 + -0.4
213,116 + +0.7
213,128 + -0.4
213,129 - +0.2
213,261 + EX28DRAFT_3549 0.12 -0.4
213,261 + EX28DRAFT_3549 0.12 +0.1

Or see this region's nucleotide sequence