Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3537 and EX28DRAFT_3538 are separated by 179 nucleotides EX28DRAFT_3538 and EX28DRAFT_3539 are separated by 17 nucleotides EX28DRAFT_3539 and EX28DRAFT_3540 are separated by 182 nucleotides
EX28DRAFT_3537: EX28DRAFT_3537 - Predicted transcriptional regulator, at 200,962 to 201,879
_3537
EX28DRAFT_3538: EX28DRAFT_3538 - 4-hydroxyphenylacetate 3-monooxygenase, reductase component, at 202,059 to 202,571
_3538
EX28DRAFT_3539: EX28DRAFT_3539 - 4-hydroxyphenylacetate 3-monooxygenase, oxygenase component, at 202,589 to 204,151
_3539
EX28DRAFT_3540: EX28DRAFT_3540 - 4-hydroxyphenylacetate catabolism regulatory protein HpaA, at 204,334 to 205,266
_3540
Position (kb) 202
203
204
205 Strain fitness (log2 ratio) -2
-1
0
1 at 201.714 kb on + strand, within EX28DRAFT_3537 at 201.742 kb on + strand, within EX28DRAFT_3537 at 201.820 kb on + strand at 201.879 kb on + strand at 201.880 kb on - strand at 201.893 kb on - strand at 201.894 kb on + strand at 201.898 kb on + strand at 201.898 kb on + strand at 201.903 kb on - strand at 201.975 kb on + strand at 201.975 kb on + strand at 201.976 kb on - strand at 202.002 kb on + strand at 202.111 kb on + strand, within EX28DRAFT_3538 at 202.111 kb on + strand, within EX28DRAFT_3538 at 202.112 kb on - strand, within EX28DRAFT_3538 at 202.145 kb on + strand, within EX28DRAFT_3538 at 202.166 kb on - strand, within EX28DRAFT_3538 at 202.166 kb on - strand, within EX28DRAFT_3538 at 202.564 kb on - strand at 202.771 kb on - strand, within EX28DRAFT_3539 at 202.978 kb on + strand, within EX28DRAFT_3539 at 202.979 kb on - strand, within EX28DRAFT_3539 at 202.979 kb on - strand, within EX28DRAFT_3539 at 203.049 kb on + strand, within EX28DRAFT_3539 at 203.050 kb on - strand, within EX28DRAFT_3539 at 203.544 kb on + strand, within EX28DRAFT_3539 at 203.628 kb on + strand, within EX28DRAFT_3539 at 203.732 kb on - strand, within EX28DRAFT_3539 at 203.757 kb on + strand, within EX28DRAFT_3539 at 203.985 kb on + strand, within EX28DRAFT_3539 at 204.057 kb on + strand at 204.057 kb on + strand at 204.061 kb on + strand at 204.061 kb on + strand at 204.063 kb on + strand at 204.063 kb on + strand at 204.063 kb on + strand at 204.063 kb on + strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.064 kb on - strand at 204.092 kb on + strand at 204.151 kb on + strand at 204.793 kb on - strand, within EX28DRAFT_3540 at 204.933 kb on - strand, within EX28DRAFT_3540
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 201,714 + EX28DRAFT_3537 0.82 +0.2 201,742 + EX28DRAFT_3537 0.85 +0.1 201,820 + -0.3 201,879 + -0.3 201,880 - +0.3 201,893 - -1.1 201,894 + -0.1 201,898 + -0.4 201,898 + +0.7 201,903 - -0.0 201,975 + -0.2 201,975 + +0.1 201,976 - -0.5 202,002 + +0.7 202,111 + EX28DRAFT_3538 0.10 -0.3 202,111 + EX28DRAFT_3538 0.10 -0.6 202,112 - EX28DRAFT_3538 0.10 +0.4 202,145 + EX28DRAFT_3538 0.17 -1.9 202,166 - EX28DRAFT_3538 0.21 -0.9 202,166 - EX28DRAFT_3538 0.21 +0.4 202,564 - -1.0 202,771 - EX28DRAFT_3539 0.12 -0.3 202,978 + EX28DRAFT_3539 0.25 -0.5 202,979 - EX28DRAFT_3539 0.25 +0.2 202,979 - EX28DRAFT_3539 0.25 -1.1 203,049 + EX28DRAFT_3539 0.29 +0.2 203,050 - EX28DRAFT_3539 0.29 -0.4 203,544 + EX28DRAFT_3539 0.61 -0.6 203,628 + EX28DRAFT_3539 0.66 +0.2 203,732 - EX28DRAFT_3539 0.73 +0.1 203,757 + EX28DRAFT_3539 0.75 -0.5 203,985 + EX28DRAFT_3539 0.89 -0.2 204,057 + -0.2 204,057 + -0.2 204,061 + -0.1 204,061 + -0.3 204,063 + +1.0 204,063 + -0.9 204,063 + +1.2 204,063 + -0.9 204,064 - -0.8 204,064 - -0.3 204,064 - -0.1 204,064 - -0.2 204,064 - -0.4 204,092 + -1.1 204,151 + -0.4 204,793 - EX28DRAFT_3540 0.49 -0.9 204,933 - EX28DRAFT_3540 0.64 -0.3
Or see this region's nucleotide sequence