Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3475 and EX28DRAFT_3476 are separated by 19 nucleotides EX28DRAFT_3476 and EX28DRAFT_3477 are separated by 53 nucleotides EX28DRAFT_3477 and EX28DRAFT_3478 are separated by 12 nucleotides
EX28DRAFT_3475: EX28DRAFT_3475 - hypothetical protein, at 143,469 to 143,654
_3475
EX28DRAFT_3476: EX28DRAFT_3476 - hypothetical protein, at 143,674 to 143,841
_3476
EX28DRAFT_3477: EX28DRAFT_3477 - Helix-turn-helix domain, at 143,895 to 144,509
_3477
EX28DRAFT_3478: EX28DRAFT_3478 - hypothetical protein, at 144,522 to 144,803
_3478
Position (kb)
143
144 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 142.714 kb on + strand at 142.714 kb on + strand at 142.715 kb on - strand at 142.777 kb on - strand at 142.786 kb on - strand at 142.786 kb on - strand at 142.832 kb on + strand at 142.832 kb on + strand at 142.832 kb on + strand at 142.833 kb on - strand at 142.870 kb on - strand at 142.873 kb on + strand at 142.873 kb on + strand at 142.873 kb on + strand at 142.873 kb on + strand at 142.874 kb on - strand at 142.874 kb on - strand at 142.874 kb on - strand at 142.914 kb on + strand at 142.915 kb on - strand at 142.924 kb on + strand at 142.924 kb on + strand at 142.924 kb on + strand at 142.925 kb on - strand at 142.925 kb on - strand at 142.925 kb on - strand at 142.932 kb on - strand at 142.934 kb on + strand at 142.934 kb on + strand at 142.943 kb on + strand at 142.944 kb on - strand at 142.944 kb on - strand at 142.990 kb on - strand at 142.990 kb on - strand at 142.995 kb on + strand at 142.996 kb on - strand at 143.006 kb on + strand at 143.014 kb on + strand at 143.054 kb on - strand at 143.057 kb on + strand at 143.058 kb on - strand at 143.060 kb on + strand at 143.073 kb on - strand at 143.073 kb on - strand at 143.089 kb on - strand at 143.089 kb on - strand at 143.172 kb on + strand at 143.209 kb on - strand at 143.233 kb on + strand at 143.234 kb on - strand at 143.234 kb on - strand at 143.234 kb on - strand at 143.349 kb on - strand at 143.351 kb on - strand at 143.355 kb on + strand at 143.356 kb on - strand at 143.356 kb on - strand at 143.356 kb on - strand at 143.359 kb on - strand at 143.366 kb on - strand at 143.368 kb on - strand at 143.445 kb on - strand at 143.545 kb on + strand, within EX28DRAFT_3475 at 143.545 kb on + strand, within EX28DRAFT_3475 at 143.789 kb on - strand, within EX28DRAFT_3476 at 143.856 kb on + strand at 143.860 kb on - strand at 143.881 kb on + strand at 144.013 kb on - strand, within EX28DRAFT_3477 at 144.033 kb on - strand, within EX28DRAFT_3477 at 144.116 kb on + strand, within EX28DRAFT_3477 at 144.142 kb on + strand, within EX28DRAFT_3477 at 144.167 kb on + strand, within EX28DRAFT_3477 at 144.173 kb on + strand, within EX28DRAFT_3477 at 144.174 kb on - strand, within EX28DRAFT_3477 at 144.174 kb on - strand, within EX28DRAFT_3477 at 144.192 kb on + strand, within EX28DRAFT_3477 at 144.193 kb on - strand, within EX28DRAFT_3477 at 144.193 kb on - strand, within EX28DRAFT_3477 at 144.220 kb on + strand, within EX28DRAFT_3477 at 144.257 kb on - strand, within EX28DRAFT_3477 at 144.321 kb on - strand, within EX28DRAFT_3477 at 144.340 kb on - strand, within EX28DRAFT_3477 at 144.421 kb on + strand, within EX28DRAFT_3477 at 144.421 kb on + strand, within EX28DRAFT_3477 at 144.475 kb on - strand at 144.539 kb on - strand at 144.572 kb on + strand, within EX28DRAFT_3478 at 144.614 kb on - strand, within EX28DRAFT_3478 at 144.668 kb on - strand, within EX28DRAFT_3478 at 144.704 kb on - strand, within EX28DRAFT_3478 at 144.751 kb on - strand, within EX28DRAFT_3478 at 144.840 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 142,714 + +0.2 142,714 + +0.5 142,715 - +0.7 142,777 - -0.2 142,786 - -0.1 142,786 - +0.6 142,832 + +0.0 142,832 + +3.9 142,832 + -0.5 142,833 - -0.3 142,870 - +0.3 142,873 + +0.6 142,873 + -0.1 142,873 + -0.3 142,873 + -0.1 142,874 - +0.5 142,874 - +0.4 142,874 - +0.1 142,914 + -0.7 142,915 - +1.0 142,924 + +0.6 142,924 + -0.3 142,924 + +0.1 142,925 - +0.1 142,925 - +1.2 142,925 - +2.3 142,932 - +0.2 142,934 + +0.1 142,934 + -0.3 142,943 + -0.1 142,944 - -0.5 142,944 - -0.3 142,990 - -0.0 142,990 - +0.7 142,995 + +0.3 142,996 - +0.7 143,006 + +0.5 143,014 + +0.2 143,054 - +2.7 143,057 + -0.4 143,058 - +0.6 143,060 + -0.2 143,073 - +0.6 143,073 - +0.2 143,089 - -0.1 143,089 - -0.3 143,172 + +0.7 143,209 - -0.3 143,233 + +0.5 143,234 - +0.5 143,234 - +1.1 143,234 - +0.0 143,349 - +0.1 143,351 - +0.2 143,355 + -0.1 143,356 - +0.3 143,356 - -0.3 143,356 - -0.8 143,359 - +0.4 143,366 - +0.1 143,368 - -0.1 143,445 - +0.2 143,545 + EX28DRAFT_3475 0.41 -1.1 143,545 + EX28DRAFT_3475 0.41 +0.2 143,789 - EX28DRAFT_3476 0.68 +0.7 143,856 + -0.0 143,860 - -0.1 143,881 + -0.5 144,013 - EX28DRAFT_3477 0.19 -0.5 144,033 - EX28DRAFT_3477 0.22 +0.4 144,116 + EX28DRAFT_3477 0.36 -0.8 144,142 + EX28DRAFT_3477 0.40 +0.7 144,167 + EX28DRAFT_3477 0.44 -0.4 144,173 + EX28DRAFT_3477 0.45 -0.4 144,174 - EX28DRAFT_3477 0.45 +0.2 144,174 - EX28DRAFT_3477 0.45 +1.6 144,192 + EX28DRAFT_3477 0.48 -0.1 144,193 - EX28DRAFT_3477 0.48 -0.8 144,193 - EX28DRAFT_3477 0.48 -0.4 144,220 + EX28DRAFT_3477 0.53 -0.2 144,257 - EX28DRAFT_3477 0.59 +0.1 144,321 - EX28DRAFT_3477 0.69 -0.3 144,340 - EX28DRAFT_3477 0.72 -0.3 144,421 + EX28DRAFT_3477 0.86 -0.4 144,421 + EX28DRAFT_3477 0.86 -0.3 144,475 - +1.0 144,539 - -0.3 144,572 + EX28DRAFT_3478 0.18 -0.1 144,614 - EX28DRAFT_3478 0.33 -0.2 144,668 - EX28DRAFT_3478 0.52 -0.3 144,704 - EX28DRAFT_3478 0.65 -1.9 144,751 - EX28DRAFT_3478 0.81 -1.1 144,840 + +0.0
Or see this region's nucleotide sequence