Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3443 and EX28DRAFT_3444 are separated by 51 nucleotides EX28DRAFT_3444 and EX28DRAFT_3445 are separated by 73 nucleotides EX28DRAFT_3445 and EX28DRAFT_3446 are separated by 12 nucleotides
EX28DRAFT_3443: EX28DRAFT_3443 - Glycosyl hydrolase family 20, catalytic domain/Glycosyl hydrolase family 20, domain 2, at 115,571 to 117,955
_3443
EX28DRAFT_3444: EX28DRAFT_3444 - reactive intermediate/imine deaminase, at 118,007 to 118,393
_3444
EX28DRAFT_3445: EX28DRAFT_3445 - aspartate carbamoyltransferase, regulatory subunit, at 118,467 to 118,928
_3445
EX28DRAFT_3446: EX28DRAFT_3446 - aspartate carbamoyltransferase (EC 2.1.3.2), at 118,941 to 119,873
_3446
Position (kb) 118
119 Strain fitness (log2 ratio) -1
0
1 at 117.350 kb on + strand, within EX28DRAFT_3443 at 117.350 kb on + strand, within EX28DRAFT_3443 at 117.351 kb on - strand, within EX28DRAFT_3443 at 117.351 kb on - strand, within EX28DRAFT_3443 at 117.504 kb on - strand, within EX28DRAFT_3443 at 117.838 kb on + strand at 117.954 kb on - strand at 118.008 kb on + strand at 118.123 kb on - strand, within EX28DRAFT_3444 at 118.123 kb on - strand, within EX28DRAFT_3444 at 118.345 kb on - strand, within EX28DRAFT_3444 at 118.435 kb on + strand at 118.436 kb on - strand at 118.437 kb on + strand at 118.437 kb on + strand at 118.438 kb on - strand at 118.510 kb on + strand at 118.942 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 117,350 + EX28DRAFT_3443 0.75 +0.5 117,350 + EX28DRAFT_3443 0.75 -0.7 117,351 - EX28DRAFT_3443 0.75 -0.4 117,351 - EX28DRAFT_3443 0.75 +0.1 117,504 - EX28DRAFT_3443 0.81 -0.5 117,838 + +0.0 117,954 - +0.0 118,008 + -0.3 118,123 - EX28DRAFT_3444 0.30 -0.5 118,123 - EX28DRAFT_3444 0.30 -0.5 118,345 - EX28DRAFT_3444 0.87 -0.8 118,435 + -0.1 118,436 - +1.0 118,437 + +0.2 118,437 + +0.3 118,438 - -0.6 118,510 + +0.1 118,942 + +0.1
Or see this region's nucleotide sequence