Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3048 and EX28DRAFT_3049 are separated by 195 nucleotides EX28DRAFT_3049 and EX28DRAFT_3050 are separated by 105 nucleotides
EX28DRAFT_3048: EX28DRAFT_3048 - AraC-type DNA-binding domain-containing proteins, at 355,088 to 355,987
_3048
EX28DRAFT_3049: EX28DRAFT_3049 - Uncharacterized oxidoreductases, Fe-dependent alcohol dehydrogenase family, at 356,183 to 357,346
_3049
EX28DRAFT_3050: EX28DRAFT_3050 - Aldo/keto reductases, related to diketogulonate reductase, at 357,452 to 358,279
_3050
Position (kb) 356
357
358 Strain fitness (log2 ratio) -1
0
1 at 355.250 kb on - strand, within EX28DRAFT_3048 at 355.250 kb on - strand, within EX28DRAFT_3048 at 355.268 kb on - strand, within EX28DRAFT_3048 at 355.268 kb on - strand, within EX28DRAFT_3048 at 355.309 kb on + strand, within EX28DRAFT_3048 at 355.597 kb on + strand, within EX28DRAFT_3048 at 355.598 kb on - strand, within EX28DRAFT_3048 at 355.748 kb on + strand, within EX28DRAFT_3048 at 355.764 kb on + strand, within EX28DRAFT_3048 at 355.802 kb on - strand, within EX28DRAFT_3048 at 355.803 kb on + strand, within EX28DRAFT_3048 at 355.988 kb on - strand at 356.195 kb on - strand at 356.288 kb on + strand at 356.510 kb on + strand, within EX28DRAFT_3049 at 356.563 kb on + strand, within EX28DRAFT_3049 at 356.724 kb on - strand, within EX28DRAFT_3049 at 356.776 kb on + strand, within EX28DRAFT_3049 at 356.790 kb on - strand, within EX28DRAFT_3049 at 356.790 kb on - strand, within EX28DRAFT_3049 at 356.799 kb on - strand, within EX28DRAFT_3049 at 356.913 kb on + strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 356.914 kb on - strand, within EX28DRAFT_3049 at 357.304 kb on + strand at 357.329 kb on + strand at 357.329 kb on + strand at 357.330 kb on - strand at 357.330 kb on - strand at 357.394 kb on + strand at 357.395 kb on - strand at 357.511 kb on - strand at 357.517 kb on - strand at 357.556 kb on + strand, within EX28DRAFT_3050 at 357.663 kb on + strand, within EX28DRAFT_3050 at 357.663 kb on + strand, within EX28DRAFT_3050 at 357.664 kb on - strand, within EX28DRAFT_3050 at 357.664 kb on - strand, within EX28DRAFT_3050 at 357.664 kb on - strand, within EX28DRAFT_3050 at 357.797 kb on + strand, within EX28DRAFT_3050 at 357.866 kb on - strand, within EX28DRAFT_3050 at 357.866 kb on - strand, within EX28DRAFT_3050 at 357.988 kb on + strand, within EX28DRAFT_3050 at 357.989 kb on - strand, within EX28DRAFT_3050 at 358.072 kb on + strand, within EX28DRAFT_3050 at 358.072 kb on + strand, within EX28DRAFT_3050 at 358.077 kb on - strand, within EX28DRAFT_3050 at 358.077 kb on - strand, within EX28DRAFT_3050 at 358.277 kb on + strand at 358.277 kb on + strand at 358.286 kb on - strand at 358.321 kb on + strand at 358.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 355,250 - EX28DRAFT_3048 0.18 -0.6 355,250 - EX28DRAFT_3048 0.18 +0.0 355,268 - EX28DRAFT_3048 0.20 -0.8 355,268 - EX28DRAFT_3048 0.20 -0.3 355,309 + EX28DRAFT_3048 0.25 +1.2 355,597 + EX28DRAFT_3048 0.57 -0.4 355,598 - EX28DRAFT_3048 0.57 +0.7 355,748 + EX28DRAFT_3048 0.73 +0.3 355,764 + EX28DRAFT_3048 0.75 -0.7 355,802 - EX28DRAFT_3048 0.79 +1.2 355,803 + EX28DRAFT_3048 0.79 -0.3 355,988 - +0.2 356,195 - +0.1 356,288 + +0.2 356,510 + EX28DRAFT_3049 0.28 -0.3 356,563 + EX28DRAFT_3049 0.33 +0.3 356,724 - EX28DRAFT_3049 0.46 -1.0 356,776 + EX28DRAFT_3049 0.51 +0.2 356,790 - EX28DRAFT_3049 0.52 -0.2 356,790 - EX28DRAFT_3049 0.52 -0.0 356,799 - EX28DRAFT_3049 0.53 -0.8 356,913 + EX28DRAFT_3049 0.63 -0.2 356,914 - EX28DRAFT_3049 0.63 +0.1 356,914 - EX28DRAFT_3049 0.63 +0.7 356,914 - EX28DRAFT_3049 0.63 -0.2 356,914 - EX28DRAFT_3049 0.63 -0.3 357,304 + +0.4 357,329 + -0.2 357,329 + +0.2 357,330 - +0.4 357,330 - -0.6 357,394 + -0.6 357,395 - -1.2 357,511 - +0.0 357,517 - -1.3 357,556 + EX28DRAFT_3050 0.13 -0.5 357,663 + EX28DRAFT_3050 0.25 +0.2 357,663 + EX28DRAFT_3050 0.25 +0.6 357,664 - EX28DRAFT_3050 0.26 -0.2 357,664 - EX28DRAFT_3050 0.26 +0.3 357,664 - EX28DRAFT_3050 0.26 -0.2 357,797 + EX28DRAFT_3050 0.42 +0.2 357,866 - EX28DRAFT_3050 0.50 -0.0 357,866 - EX28DRAFT_3050 0.50 -0.0 357,988 + EX28DRAFT_3050 0.65 +0.2 357,989 - EX28DRAFT_3050 0.65 -0.6 358,072 + EX28DRAFT_3050 0.75 +0.5 358,072 + EX28DRAFT_3050 0.75 -0.7 358,077 - EX28DRAFT_3050 0.75 -1.2 358,077 - EX28DRAFT_3050 0.75 +0.6 358,277 + +0.4 358,277 + +0.1 358,286 - -0.2 358,321 + +0.7 358,322 - -0.7
Or see this region's nucleotide sequence