Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2803 and EX28DRAFT_2804 are separated by 5 nucleotides EX28DRAFT_2804 and EX28DRAFT_2805 are separated by 134 nucleotides EX28DRAFT_2805 and EX28DRAFT_2806 are separated by 214 nucleotides
EX28DRAFT_2803: EX28DRAFT_2803 - [NiFe] hydrogenase maturation protein HypF, at 105,712 to 107,928
_2803
EX28DRAFT_2804: EX28DRAFT_2804 - Fe-S-cluster-containing hydrogenase components 2, at 107,934 to 108,479
_2804
EX28DRAFT_2805: EX28DRAFT_2805 - Transcriptional regulators, at 108,614 to 109,627
_2805
EX28DRAFT_2806: EX28DRAFT_2806 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 109,842 to 111,293
_2806
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 107.673 kb on + strand, within EX28DRAFT_2803 at 107.673 kb on + strand, within EX28DRAFT_2803 at 107.674 kb on - strand, within EX28DRAFT_2803 at 107.911 kb on + strand at 107.912 kb on - strand at 107.928 kb on + strand at 107.928 kb on + strand at 108.117 kb on + strand, within EX28DRAFT_2804 at 108.117 kb on + strand, within EX28DRAFT_2804 at 108.118 kb on - strand, within EX28DRAFT_2804 at 108.145 kb on - strand, within EX28DRAFT_2804 at 108.145 kb on - strand, within EX28DRAFT_2804 at 108.147 kb on + strand, within EX28DRAFT_2804 at 108.229 kb on - strand, within EX28DRAFT_2804 at 108.229 kb on - strand, within EX28DRAFT_2804 at 108.229 kb on - strand, within EX28DRAFT_2804 at 108.254 kb on + strand, within EX28DRAFT_2804 at 108.320 kb on + strand, within EX28DRAFT_2804 at 108.327 kb on - strand, within EX28DRAFT_2804 at 108.432 kb on - strand at 108.449 kb on + strand at 108.450 kb on - strand at 108.467 kb on + strand at 108.468 kb on - strand at 108.468 kb on - strand at 108.495 kb on + strand at 108.496 kb on - strand at 108.502 kb on - strand at 108.591 kb on + strand at 108.591 kb on + strand at 108.591 kb on + strand at 108.592 kb on - strand at 108.682 kb on + strand at 108.682 kb on + strand at 108.682 kb on + strand at 108.682 kb on + strand at 108.752 kb on - strand, within EX28DRAFT_2805 at 108.780 kb on + strand, within EX28DRAFT_2805 at 108.781 kb on - strand, within EX28DRAFT_2805 at 109.075 kb on + strand, within EX28DRAFT_2805 at 109.258 kb on - strand, within EX28DRAFT_2805 at 109.404 kb on + strand, within EX28DRAFT_2805 at 109.404 kb on + strand, within EX28DRAFT_2805 at 109.417 kb on - strand, within EX28DRAFT_2805 at 109.426 kb on + strand, within EX28DRAFT_2805 at 109.426 kb on + strand, within EX28DRAFT_2805 at 109.427 kb on - strand, within EX28DRAFT_2805 at 109.427 kb on - strand, within EX28DRAFT_2805 at 109.549 kb on - strand at 109.549 kb on - strand at 109.628 kb on - strand at 109.630 kb on + strand at 109.630 kb on + strand at 109.631 kb on - strand at 109.631 kb on - strand at 109.631 kb on - strand at 109.663 kb on + strand at 110.137 kb on + strand, within EX28DRAFT_2806 at 110.258 kb on - strand, within EX28DRAFT_2806 at 110.491 kb on - strand, within EX28DRAFT_2806 at 110.538 kb on + strand, within EX28DRAFT_2806 at 110.538 kb on + strand, within EX28DRAFT_2806
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 107,673 + EX28DRAFT_2803 0.88 +0.8 107,673 + EX28DRAFT_2803 0.88 +0.4 107,674 - EX28DRAFT_2803 0.88 +0.9 107,911 + -0.1 107,912 - +0.5 107,928 + +0.4 107,928 + -0.3 108,117 + EX28DRAFT_2804 0.34 -1.0 108,117 + EX28DRAFT_2804 0.34 +0.0 108,118 - EX28DRAFT_2804 0.34 -0.2 108,145 - EX28DRAFT_2804 0.39 -0.1 108,145 - EX28DRAFT_2804 0.39 -0.3 108,147 + EX28DRAFT_2804 0.39 +0.5 108,229 - EX28DRAFT_2804 0.54 -1.0 108,229 - EX28DRAFT_2804 0.54 +0.6 108,229 - EX28DRAFT_2804 0.54 +0.2 108,254 + EX28DRAFT_2804 0.59 -0.5 108,320 + EX28DRAFT_2804 0.71 -1.4 108,327 - EX28DRAFT_2804 0.72 -1.0 108,432 - +0.2 108,449 + +0.6 108,450 - -0.3 108,467 + +0.4 108,468 - +0.8 108,468 - +0.1 108,495 + -0.5 108,496 - +0.4 108,502 - +1.1 108,591 + +0.1 108,591 + +0.2 108,591 + -0.0 108,592 - -0.4 108,682 + +0.0 108,682 + +0.4 108,682 + +0.2 108,682 + -1.0 108,752 - EX28DRAFT_2805 0.14 -0.5 108,780 + EX28DRAFT_2805 0.16 -1.2 108,781 - EX28DRAFT_2805 0.16 -1.8 109,075 + EX28DRAFT_2805 0.45 -0.8 109,258 - EX28DRAFT_2805 0.64 -0.8 109,404 + EX28DRAFT_2805 0.78 -0.0 109,404 + EX28DRAFT_2805 0.78 -0.5 109,417 - EX28DRAFT_2805 0.79 +0.9 109,426 + EX28DRAFT_2805 0.80 -0.9 109,426 + EX28DRAFT_2805 0.80 -0.7 109,427 - EX28DRAFT_2805 0.80 -0.7 109,427 - EX28DRAFT_2805 0.80 -0.8 109,549 - -0.4 109,549 - +0.8 109,628 - -0.2 109,630 + -0.2 109,630 + +0.1 109,631 - +0.1 109,631 - +0.4 109,631 - +2.0 109,663 + +0.1 110,137 + EX28DRAFT_2806 0.20 +0.4 110,258 - EX28DRAFT_2806 0.29 -0.7 110,491 - EX28DRAFT_2806 0.45 +0.0 110,538 + EX28DRAFT_2806 0.48 +0.0 110,538 + EX28DRAFT_2806 0.48 +0.6
Or see this region's nucleotide sequence