Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2451 and EX28DRAFT_2452 are separated by 86 nucleotides EX28DRAFT_2452 and EX28DRAFT_2453 are separated by 74 nucleotides EX28DRAFT_2453 and EX28DRAFT_2454 are separated by 92 nucleotides
EX28DRAFT_2451: EX28DRAFT_2451 - PTS system, glucose-like IIB component, at 2,481,290 to 2,482,789
_2451
EX28DRAFT_2452: EX28DRAFT_2452 - transcriptional regulator, LacI family, at 2,482,876 to 2,483,892
_2452
EX28DRAFT_2453: EX28DRAFT_2453 - Predicted membrane-bound metal-dependent hydrolases, at 2,483,967 to 2,484,488
_2453
EX28DRAFT_2454: EX28DRAFT_2454 - cysteinyl-tRNA synthetase (EC 6.1.1.16), at 2,484,581 to 2,485,966
_2454
Position (kb)
2482
2483
2484 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2481.972 kb on - strand, within EX28DRAFT_2451 at 2482.064 kb on + strand, within EX28DRAFT_2451 at 2482.064 kb on + strand, within EX28DRAFT_2451 at 2482.142 kb on + strand, within EX28DRAFT_2451 at 2482.243 kb on + strand, within EX28DRAFT_2451 at 2482.304 kb on + strand, within EX28DRAFT_2451 at 2482.304 kb on + strand, within EX28DRAFT_2451 at 2482.305 kb on - strand, within EX28DRAFT_2451 at 2482.305 kb on - strand, within EX28DRAFT_2451 at 2482.305 kb on - strand, within EX28DRAFT_2451 at 2482.316 kb on + strand, within EX28DRAFT_2451 at 2482.316 kb on + strand, within EX28DRAFT_2451 at 2482.316 kb on + strand, within EX28DRAFT_2451 at 2482.316 kb on + strand, within EX28DRAFT_2451 at 2482.317 kb on - strand, within EX28DRAFT_2451 at 2482.317 kb on - strand, within EX28DRAFT_2451 at 2482.367 kb on + strand, within EX28DRAFT_2451 at 2482.458 kb on - strand, within EX28DRAFT_2451 at 2482.787 kb on + strand at 2482.790 kb on + strand at 2482.791 kb on - strand at 2482.791 kb on - strand at 2483.014 kb on + strand, within EX28DRAFT_2452 at 2483.022 kb on + strand, within EX28DRAFT_2452 at 2483.149 kb on + strand, within EX28DRAFT_2452 at 2483.151 kb on + strand, within EX28DRAFT_2452 at 2483.152 kb on - strand, within EX28DRAFT_2452 at 2483.214 kb on + strand, within EX28DRAFT_2452 at 2483.243 kb on - strand, within EX28DRAFT_2452 at 2483.392 kb on + strand, within EX28DRAFT_2452 at 2483.426 kb on - strand, within EX28DRAFT_2452 at 2483.521 kb on + strand, within EX28DRAFT_2452 at 2483.724 kb on + strand, within EX28DRAFT_2452 at 2483.725 kb on - strand, within EX28DRAFT_2452 at 2483.725 kb on - strand, within EX28DRAFT_2452 at 2483.761 kb on + strand, within EX28DRAFT_2452 at 2483.762 kb on - strand, within EX28DRAFT_2452 at 2483.762 kb on - strand, within EX28DRAFT_2452 at 2483.762 kb on - strand, within EX28DRAFT_2452 at 2483.762 kb on - strand, within EX28DRAFT_2452 at 2483.937 kb on + strand at 2483.981 kb on + strand at 2484.014 kb on - strand at 2484.172 kb on + strand, within EX28DRAFT_2453 at 2484.172 kb on + strand, within EX28DRAFT_2453 at 2484.360 kb on - strand, within EX28DRAFT_2453 at 2484.388 kb on - strand, within EX28DRAFT_2453 at 2484.487 kb on - strand at 2484.582 kb on + strand at 2484.583 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,481,972 - EX28DRAFT_2451 0.45 -0.2 2,482,064 + EX28DRAFT_2451 0.52 -0.5 2,482,064 + EX28DRAFT_2451 0.52 -0.4 2,482,142 + EX28DRAFT_2451 0.57 +2.7 2,482,243 + EX28DRAFT_2451 0.64 +0.0 2,482,304 + EX28DRAFT_2451 0.68 -2.9 2,482,304 + EX28DRAFT_2451 0.68 -1.4 2,482,305 - EX28DRAFT_2451 0.68 -0.4 2,482,305 - EX28DRAFT_2451 0.68 +0.2 2,482,305 - EX28DRAFT_2451 0.68 -1.6 2,482,316 + EX28DRAFT_2451 0.68 +0.7 2,482,316 + EX28DRAFT_2451 0.68 +0.2 2,482,316 + EX28DRAFT_2451 0.68 -0.3 2,482,316 + EX28DRAFT_2451 0.68 -0.1 2,482,317 - EX28DRAFT_2451 0.68 -0.1 2,482,317 - EX28DRAFT_2451 0.68 -0.1 2,482,367 + EX28DRAFT_2451 0.72 +0.7 2,482,458 - EX28DRAFT_2451 0.78 -0.1 2,482,787 + +0.5 2,482,790 + -1.0 2,482,791 - +0.3 2,482,791 - -0.1 2,483,014 + EX28DRAFT_2452 0.14 -1.5 2,483,022 + EX28DRAFT_2452 0.14 +1.6 2,483,149 + EX28DRAFT_2452 0.27 -0.2 2,483,151 + EX28DRAFT_2452 0.27 +0.6 2,483,152 - EX28DRAFT_2452 0.27 -0.3 2,483,214 + EX28DRAFT_2452 0.33 +0.3 2,483,243 - EX28DRAFT_2452 0.36 -0.3 2,483,392 + EX28DRAFT_2452 0.51 -0.4 2,483,426 - EX28DRAFT_2452 0.54 -0.2 2,483,521 + EX28DRAFT_2452 0.63 -2.6 2,483,724 + EX28DRAFT_2452 0.83 +0.6 2,483,725 - EX28DRAFT_2452 0.83 -0.2 2,483,725 - EX28DRAFT_2452 0.83 -2.8 2,483,761 + EX28DRAFT_2452 0.87 -0.3 2,483,762 - EX28DRAFT_2452 0.87 -0.0 2,483,762 - EX28DRAFT_2452 0.87 -0.3 2,483,762 - EX28DRAFT_2452 0.87 +0.6 2,483,762 - EX28DRAFT_2452 0.87 -1.0 2,483,937 + +1.2 2,483,981 + -0.3 2,484,014 - -0.1 2,484,172 + EX28DRAFT_2453 0.39 -0.4 2,484,172 + EX28DRAFT_2453 0.39 +0.2 2,484,360 - EX28DRAFT_2453 0.75 +0.6 2,484,388 - EX28DRAFT_2453 0.81 -0.0 2,484,487 - +0.6 2,484,582 + +0.4 2,484,583 - +3.0
Or see this region's nucleotide sequence