Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2105 and EX28DRAFT_2106 are separated by 79 nucleotides EX28DRAFT_2106 and EX28DRAFT_2107 are separated by 174 nucleotides EX28DRAFT_2107 and EX28DRAFT_2108 are separated by 48 nucleotides EX28DRAFT_2108 and EX28DRAFT_2109 are separated by 19 nucleotides
EX28DRAFT_2105: EX28DRAFT_2105 - Predicted permease, at 2,127,772 to 2,129,457
_2105
EX28DRAFT_2106: EX28DRAFT_2106 - RNA, at 2,129,537 to 2,129,615
_2106
EX28DRAFT_2107: EX28DRAFT_2107 - hypothetical protein, at 2,129,790 to 2,130,389
_2107
EX28DRAFT_2108: EX28DRAFT_2108 - phage portal protein, PBSX family, at 2,130,438 to 2,131,037
_2108
EX28DRAFT_2109: EX28DRAFT_2109 - Reverse transcriptase (RNA-dependent DNA polymerase), at 2,131,057 to 2,132,040
_2109
Position (kb)
2130
2131
2132 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2129.510 kb on + strand at 2129.526 kb on + strand at 2129.526 kb on + strand at 2129.527 kb on - strand at 2129.530 kb on + strand at 2129.833 kb on + strand at 2129.833 kb on + strand at 2129.834 kb on - strand at 2129.834 kb on - strand at 2129.874 kb on + strand, within EX28DRAFT_2107 at 2129.889 kb on - strand, within EX28DRAFT_2107 at 2129.952 kb on + strand, within EX28DRAFT_2107 at 2129.953 kb on - strand, within EX28DRAFT_2107 at 2129.961 kb on - strand, within EX28DRAFT_2107 at 2129.987 kb on + strand, within EX28DRAFT_2107 at 2130.028 kb on - strand, within EX28DRAFT_2107 at 2130.076 kb on + strand, within EX28DRAFT_2107 at 2130.088 kb on + strand, within EX28DRAFT_2107 at 2130.088 kb on + strand, within EX28DRAFT_2107 at 2130.089 kb on - strand, within EX28DRAFT_2107 at 2130.138 kb on - strand, within EX28DRAFT_2107 at 2130.138 kb on - strand, within EX28DRAFT_2107 at 2130.376 kb on + strand at 2130.376 kb on + strand at 2130.420 kb on + strand at 2130.425 kb on + strand at 2130.425 kb on + strand at 2130.426 kb on - strand at 2130.471 kb on - strand at 2130.483 kb on + strand at 2130.484 kb on - strand at 2130.558 kb on + strand, within EX28DRAFT_2108 at 2130.559 kb on - strand, within EX28DRAFT_2108 at 2130.618 kb on + strand, within EX28DRAFT_2108 at 2130.618 kb on + strand, within EX28DRAFT_2108 at 2130.622 kb on - strand, within EX28DRAFT_2108 at 2130.622 kb on - strand, within EX28DRAFT_2108 at 2130.648 kb on - strand, within EX28DRAFT_2108 at 2130.648 kb on - strand, within EX28DRAFT_2108 at 2130.654 kb on + strand, within EX28DRAFT_2108 at 2130.654 kb on + strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.655 kb on - strand, within EX28DRAFT_2108 at 2130.656 kb on + strand, within EX28DRAFT_2108 at 2130.657 kb on - strand, within EX28DRAFT_2108 at 2130.780 kb on - strand, within EX28DRAFT_2108 at 2130.815 kb on + strand, within EX28DRAFT_2108 at 2130.819 kb on + strand, within EX28DRAFT_2108 at 2130.844 kb on - strand, within EX28DRAFT_2108 at 2130.894 kb on + strand, within EX28DRAFT_2108 at 2131.010 kb on - strand at 2131.025 kb on + strand at 2131.082 kb on - strand at 2131.254 kb on + strand, within EX28DRAFT_2109 at 2131.258 kb on + strand, within EX28DRAFT_2109 at 2131.258 kb on + strand, within EX28DRAFT_2109 at 2131.506 kb on + strand, within EX28DRAFT_2109 at 2131.576 kb on - strand, within EX28DRAFT_2109
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,129,510 + +1.0 2,129,526 + -1.6 2,129,526 + +0.2 2,129,527 - +0.8 2,129,530 + +0.4 2,129,833 + -0.0 2,129,833 + -1.8 2,129,834 - -0.5 2,129,834 - +0.3 2,129,874 + EX28DRAFT_2107 0.14 +0.4 2,129,889 - EX28DRAFT_2107 0.17 -0.5 2,129,952 + EX28DRAFT_2107 0.27 -0.1 2,129,953 - EX28DRAFT_2107 0.27 -1.6 2,129,961 - EX28DRAFT_2107 0.28 +0.4 2,129,987 + EX28DRAFT_2107 0.33 +2.2 2,130,028 - EX28DRAFT_2107 0.40 +2.7 2,130,076 + EX28DRAFT_2107 0.48 -0.2 2,130,088 + EX28DRAFT_2107 0.50 +1.4 2,130,088 + EX28DRAFT_2107 0.50 +0.4 2,130,089 - EX28DRAFT_2107 0.50 +0.4 2,130,138 - EX28DRAFT_2107 0.58 +1.0 2,130,138 - EX28DRAFT_2107 0.58 -1.0 2,130,376 + -0.6 2,130,376 + +2.0 2,130,420 + -0.4 2,130,425 + +1.6 2,130,425 + -0.2 2,130,426 - +0.9 2,130,471 - +2.0 2,130,483 + +0.3 2,130,484 - -0.8 2,130,558 + EX28DRAFT_2108 0.20 -0.5 2,130,559 - EX28DRAFT_2108 0.20 +0.8 2,130,618 + EX28DRAFT_2108 0.30 -1.5 2,130,618 + EX28DRAFT_2108 0.30 +3.6 2,130,622 - EX28DRAFT_2108 0.31 -1.0 2,130,622 - EX28DRAFT_2108 0.31 +1.8 2,130,648 - EX28DRAFT_2108 0.35 +0.2 2,130,648 - EX28DRAFT_2108 0.35 -0.9 2,130,654 + EX28DRAFT_2108 0.36 +0.7 2,130,654 + EX28DRAFT_2108 0.36 -0.6 2,130,655 - EX28DRAFT_2108 0.36 -0.1 2,130,655 - EX28DRAFT_2108 0.36 +0.8 2,130,655 - EX28DRAFT_2108 0.36 -1.5 2,130,655 - EX28DRAFT_2108 0.36 -0.4 2,130,655 - EX28DRAFT_2108 0.36 +0.1 2,130,655 - EX28DRAFT_2108 0.36 -0.3 2,130,656 + EX28DRAFT_2108 0.36 +0.0 2,130,657 - EX28DRAFT_2108 0.36 +0.1 2,130,780 - EX28DRAFT_2108 0.57 -1.1 2,130,815 + EX28DRAFT_2108 0.63 -0.4 2,130,819 + EX28DRAFT_2108 0.64 -0.3 2,130,844 - EX28DRAFT_2108 0.68 +0.6 2,130,894 + EX28DRAFT_2108 0.76 -0.1 2,131,010 - -0.8 2,131,025 + +0.8 2,131,082 - +0.1 2,131,254 + EX28DRAFT_2109 0.20 +0.0 2,131,258 + EX28DRAFT_2109 0.20 -1.3 2,131,258 + EX28DRAFT_2109 0.20 +0.2 2,131,506 + EX28DRAFT_2109 0.46 -0.1 2,131,576 - EX28DRAFT_2109 0.53 -1.9
Or see this region's nucleotide sequence