Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2001 and EX28DRAFT_2002 are separated by 9 nucleotides EX28DRAFT_2002 and EX28DRAFT_2003 overlap by 4 nucleotides EX28DRAFT_2003 and EX28DRAFT_2004 are separated by 51 nucleotides
EX28DRAFT_2001: EX28DRAFT_2001 - alkanesulfonate monooxygenase (EC 1.14.14.5), at 2,004,421 to 2,005,566
_2001
EX28DRAFT_2002: EX28DRAFT_2002 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 2,005,576 to 2,006,367
_2002
EX28DRAFT_2003: EX28DRAFT_2003 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 2,006,364 to 2,007,134
_2003
EX28DRAFT_2004: EX28DRAFT_2004 - aminopeptidase N, Escherichia coli type, at 2,007,186 to 2,009,798
_2004
Position (kb)
2005
2006
2007 Strain fitness (log2 ratio)
-1
0
1
2 at 2004.796 kb on - strand, within EX28DRAFT_2001 at 2004.805 kb on + strand, within EX28DRAFT_2001 at 2004.882 kb on + strand, within EX28DRAFT_2001 at 2004.882 kb on + strand, within EX28DRAFT_2001 at 2004.883 kb on - strand, within EX28DRAFT_2001 at 2004.908 kb on - strand, within EX28DRAFT_2001 at 2004.908 kb on - strand, within EX28DRAFT_2001 at 2004.944 kb on - strand, within EX28DRAFT_2001 at 2005.000 kb on + strand, within EX28DRAFT_2001 at 2005.401 kb on + strand, within EX28DRAFT_2001 at 2005.565 kb on - strand at 2005.712 kb on + strand, within EX28DRAFT_2002 at 2005.993 kb on + strand, within EX28DRAFT_2002 at 2005.994 kb on - strand, within EX28DRAFT_2002 at 2006.063 kb on + strand, within EX28DRAFT_2002 at 2006.082 kb on + strand, within EX28DRAFT_2002 at 2006.083 kb on - strand, within EX28DRAFT_2002 at 2006.084 kb on + strand, within EX28DRAFT_2002 at 2006.085 kb on - strand, within EX28DRAFT_2002 at 2006.118 kb on + strand, within EX28DRAFT_2002 at 2006.199 kb on - strand, within EX28DRAFT_2002 at 2006.199 kb on - strand, within EX28DRAFT_2002 at 2006.266 kb on + strand, within EX28DRAFT_2002 at 2006.267 kb on - strand, within EX28DRAFT_2002 at 2006.341 kb on + strand at 2006.341 kb on + strand at 2006.341 kb on + strand at 2006.341 kb on + strand at 2006.370 kb on - strand at 2006.370 kb on - strand at 2006.462 kb on + strand, within EX28DRAFT_2003 at 2006.678 kb on + strand, within EX28DRAFT_2003 at 2006.980 kb on + strand, within EX28DRAFT_2003 at 2006.995 kb on + strand, within EX28DRAFT_2003 at 2007.109 kb on + strand at 2007.119 kb on + strand at 2007.119 kb on + strand at 2007.119 kb on + strand at 2007.120 kb on - strand at 2007.120 kb on - strand at 2007.175 kb on + strand at 2007.176 kb on - strand at 2007.185 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,004,796 - EX28DRAFT_2001 0.33 -0.3 2,004,805 + EX28DRAFT_2001 0.34 +0.4 2,004,882 + EX28DRAFT_2001 0.40 +0.1 2,004,882 + EX28DRAFT_2001 0.40 -0.2 2,004,883 - EX28DRAFT_2001 0.40 +0.7 2,004,908 - EX28DRAFT_2001 0.42 +2.2 2,004,908 - EX28DRAFT_2001 0.42 -0.4 2,004,944 - EX28DRAFT_2001 0.46 +0.8 2,005,000 + EX28DRAFT_2001 0.51 +0.5 2,005,401 + EX28DRAFT_2001 0.86 -0.6 2,005,565 - -0.1 2,005,712 + EX28DRAFT_2002 0.17 +0.3 2,005,993 + EX28DRAFT_2002 0.53 +1.0 2,005,994 - EX28DRAFT_2002 0.53 +0.1 2,006,063 + EX28DRAFT_2002 0.61 +2.7 2,006,082 + EX28DRAFT_2002 0.64 +0.7 2,006,083 - EX28DRAFT_2002 0.64 +0.0 2,006,084 + EX28DRAFT_2002 0.64 -1.0 2,006,085 - EX28DRAFT_2002 0.64 -0.5 2,006,118 + EX28DRAFT_2002 0.68 -0.8 2,006,199 - EX28DRAFT_2002 0.79 +0.1 2,006,199 - EX28DRAFT_2002 0.79 +0.9 2,006,266 + EX28DRAFT_2002 0.87 +0.3 2,006,267 - EX28DRAFT_2002 0.87 +0.2 2,006,341 + +0.9 2,006,341 + +0.6 2,006,341 + -0.1 2,006,341 + -0.0 2,006,370 - -0.8 2,006,370 - +0.1 2,006,462 + EX28DRAFT_2003 0.13 +0.8 2,006,678 + EX28DRAFT_2003 0.41 +0.7 2,006,980 + EX28DRAFT_2003 0.80 +0.0 2,006,995 + EX28DRAFT_2003 0.82 +0.2 2,007,109 + +0.5 2,007,119 + +0.4 2,007,119 + +0.2 2,007,119 + +0.6 2,007,120 - -0.1 2,007,120 - -0.1 2,007,175 + -0.4 2,007,176 - +1.4 2,007,185 - +0.3
Or see this region's nucleotide sequence