Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1779 and EX28DRAFT_1780 overlap by 17 nucleotides EX28DRAFT_1780 and EX28DRAFT_1781 are separated by 99 nucleotides EX28DRAFT_1781 and EX28DRAFT_1782 are separated by 32 nucleotides EX28DRAFT_1782 and EX28DRAFT_1783 are separated by 101 nucleotides
EX28DRAFT_1779: EX28DRAFT_1779 - Predicted membrane protein, at 1,795,762 to 1,796,208
_1779
EX28DRAFT_1780: EX28DRAFT_1780 - AraC-type DNA-binding domain-containing proteins, at 1,796,192 to 1,796,983
_1780
EX28DRAFT_1781: EX28DRAFT_1781 - cyanate transporter, at 1,797,083 to 1,798,270
_1781
EX28DRAFT_1782: EX28DRAFT_1782 - Glutamine amidotransferase class-I, at 1,798,303 to 1,799,010
_1782
EX28DRAFT_1783: EX28DRAFT_1783 - Uncharacterized conserved protein, at 1,799,112 to 1,799,456
_1783
Position (kb)
1797
1798
1799 Strain fitness (log2 ratio)
-1
0
1
2 at 1796.206 kb on + strand at 1796.207 kb on - strand at 1796.222 kb on - strand at 1796.223 kb on + strand at 1796.223 kb on + strand at 1796.224 kb on - strand at 1796.267 kb on - strand at 1796.278 kb on + strand, within EX28DRAFT_1780 at 1796.278 kb on + strand, within EX28DRAFT_1780 at 1796.339 kb on + strand, within EX28DRAFT_1780 at 1796.339 kb on + strand, within EX28DRAFT_1780 at 1796.339 kb on + strand, within EX28DRAFT_1780 at 1796.513 kb on - strand, within EX28DRAFT_1780 at 1796.890 kb on + strand, within EX28DRAFT_1780 at 1796.892 kb on + strand, within EX28DRAFT_1780 at 1796.892 kb on + strand, within EX28DRAFT_1780 at 1796.893 kb on - strand, within EX28DRAFT_1780 at 1796.893 kb on - strand, within EX28DRAFT_1780 at 1796.959 kb on - strand at 1797.081 kb on + strand at 1797.081 kb on + strand at 1797.082 kb on - strand at 1797.088 kb on + strand at 1797.212 kb on + strand, within EX28DRAFT_1781 at 1797.460 kb on + strand, within EX28DRAFT_1781 at 1797.504 kb on - strand, within EX28DRAFT_1781 at 1797.504 kb on - strand, within EX28DRAFT_1781 at 1797.761 kb on + strand, within EX28DRAFT_1781 at 1797.970 kb on + strand, within EX28DRAFT_1781 at 1797.971 kb on - strand, within EX28DRAFT_1781 at 1798.461 kb on + strand, within EX28DRAFT_1782 at 1798.462 kb on - strand, within EX28DRAFT_1782 at 1798.462 kb on - strand, within EX28DRAFT_1782 at 1798.506 kb on - strand, within EX28DRAFT_1782 at 1798.506 kb on - strand, within EX28DRAFT_1782 at 1798.506 kb on - strand, within EX28DRAFT_1782 at 1798.506 kb on - strand, within EX28DRAFT_1782 at 1798.506 kb on - strand, within EX28DRAFT_1782 at 1798.506 kb on - strand, within EX28DRAFT_1782 at 1798.509 kb on + strand, within EX28DRAFT_1782 at 1798.510 kb on - strand, within EX28DRAFT_1782 at 1798.510 kb on - strand, within EX28DRAFT_1782 at 1798.564 kb on - strand, within EX28DRAFT_1782 at 1798.691 kb on + strand, within EX28DRAFT_1782 at 1798.691 kb on + strand, within EX28DRAFT_1782 at 1798.792 kb on - strand, within EX28DRAFT_1782 at 1798.794 kb on - strand, within EX28DRAFT_1782 at 1798.944 kb on + strand at 1798.959 kb on - strand at 1799.035 kb on + strand at 1799.036 kb on - strand at 1799.048 kb on - strand at 1799.048 kb on - strand at 1799.048 kb on - strand at 1799.092 kb on + strand at 1799.092 kb on + strand at 1799.111 kb on + strand at 1799.112 kb on - strand at 1799.112 kb on - strand at 1799.132 kb on - strand at 1799.133 kb on + strand at 1799.257 kb on + strand, within EX28DRAFT_1783 at 1799.258 kb on - strand, within EX28DRAFT_1783 at 1799.258 kb on - strand, within EX28DRAFT_1783 at 1799.262 kb on - strand, within EX28DRAFT_1783
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,796,206 + -0.1 1,796,207 - -0.2 1,796,222 - -0.1 1,796,223 + -1.1 1,796,223 + +0.6 1,796,224 - -0.4 1,796,267 - +0.3 1,796,278 + EX28DRAFT_1780 0.11 -0.3 1,796,278 + EX28DRAFT_1780 0.11 +0.4 1,796,339 + EX28DRAFT_1780 0.19 -0.5 1,796,339 + EX28DRAFT_1780 0.19 +1.4 1,796,339 + EX28DRAFT_1780 0.19 +0.8 1,796,513 - EX28DRAFT_1780 0.41 +0.3 1,796,890 + EX28DRAFT_1780 0.88 +0.7 1,796,892 + EX28DRAFT_1780 0.88 -0.2 1,796,892 + EX28DRAFT_1780 0.88 +0.0 1,796,893 - EX28DRAFT_1780 0.89 -0.0 1,796,893 - EX28DRAFT_1780 0.89 +0.5 1,796,959 - +1.2 1,797,081 + +1.8 1,797,081 + +0.2 1,797,082 - -0.6 1,797,088 + +0.4 1,797,212 + EX28DRAFT_1781 0.11 -0.4 1,797,460 + EX28DRAFT_1781 0.32 +0.0 1,797,504 - EX28DRAFT_1781 0.35 -1.1 1,797,504 - EX28DRAFT_1781 0.35 -1.2 1,797,761 + EX28DRAFT_1781 0.57 +0.2 1,797,970 + EX28DRAFT_1781 0.75 -0.5 1,797,971 - EX28DRAFT_1781 0.75 +0.3 1,798,461 + EX28DRAFT_1782 0.22 +1.1 1,798,462 - EX28DRAFT_1782 0.22 -0.6 1,798,462 - EX28DRAFT_1782 0.22 +0.2 1,798,506 - EX28DRAFT_1782 0.29 -0.4 1,798,506 - EX28DRAFT_1782 0.29 -0.1 1,798,506 - EX28DRAFT_1782 0.29 +0.3 1,798,506 - EX28DRAFT_1782 0.29 +0.5 1,798,506 - EX28DRAFT_1782 0.29 +0.4 1,798,506 - EX28DRAFT_1782 0.29 -0.2 1,798,509 + EX28DRAFT_1782 0.29 +1.0 1,798,510 - EX28DRAFT_1782 0.29 +0.2 1,798,510 - EX28DRAFT_1782 0.29 -0.4 1,798,564 - EX28DRAFT_1782 0.37 -0.1 1,798,691 + EX28DRAFT_1782 0.55 +0.3 1,798,691 + EX28DRAFT_1782 0.55 +0.2 1,798,792 - EX28DRAFT_1782 0.69 -0.9 1,798,794 - EX28DRAFT_1782 0.69 -1.0 1,798,944 + +0.5 1,798,959 - +0.6 1,799,035 + +0.8 1,799,036 - +2.2 1,799,048 - +0.2 1,799,048 - -0.2 1,799,048 - +0.8 1,799,092 + +1.2 1,799,092 + -0.2 1,799,111 + +0.2 1,799,112 - -0.1 1,799,112 - -0.1 1,799,132 - +1.0 1,799,133 + -1.1 1,799,257 + EX28DRAFT_1783 0.42 +0.7 1,799,258 - EX28DRAFT_1783 0.42 +0.4 1,799,258 - EX28DRAFT_1783 0.42 -0.1 1,799,262 - EX28DRAFT_1783 0.43 +1.2
Or see this region's nucleotide sequence