Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0164 and EX28DRAFT_0165 are separated by 4 nucleotides EX28DRAFT_0165 and EX28DRAFT_0166 are separated by 3 nucleotides
EX28DRAFT_0164: EX28DRAFT_0164 - FOG: EAL domain, at 110,002 to 112,242
_0164
EX28DRAFT_0165: EX28DRAFT_0165 - exopolyphosphatase, at 112,247 to 113,785
_0165
EX28DRAFT_0166: EX28DRAFT_0166 - polyphosphate kinase 1, at 113,789 to 115,849
_0166
Position (kb) 112
113
114 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 111.334 kb on + strand, within EX28DRAFT_0164 at 111.335 kb on - strand, within EX28DRAFT_0164 at 111.335 kb on - strand, within EX28DRAFT_0164 at 111.335 kb on - strand, within EX28DRAFT_0164 at 111.335 kb on - strand, within EX28DRAFT_0164 at 111.340 kb on + strand, within EX28DRAFT_0164 at 111.340 kb on + strand, within EX28DRAFT_0164 at 111.341 kb on - strand, within EX28DRAFT_0164 at 111.341 kb on - strand, within EX28DRAFT_0164 at 111.367 kb on + strand, within EX28DRAFT_0164 at 111.602 kb on + strand, within EX28DRAFT_0164 at 111.817 kb on + strand, within EX28DRAFT_0164 at 111.818 kb on - strand, within EX28DRAFT_0164 at 111.891 kb on - strand, within EX28DRAFT_0164 at 111.927 kb on + strand, within EX28DRAFT_0164 at 111.928 kb on - strand, within EX28DRAFT_0164 at 111.928 kb on - strand, within EX28DRAFT_0164 at 111.964 kb on + strand, within EX28DRAFT_0164 at 111.964 kb on + strand, within EX28DRAFT_0164 at 111.965 kb on - strand, within EX28DRAFT_0164 at 112.112 kb on - strand at 112.130 kb on + strand at 112.130 kb on + strand at 112.131 kb on - strand at 112.131 kb on - strand at 112.135 kb on - strand at 112.162 kb on + strand at 112.162 kb on + strand at 112.163 kb on - strand at 112.627 kb on - strand, within EX28DRAFT_0165 at 112.755 kb on + strand, within EX28DRAFT_0165 at 112.756 kb on - strand, within EX28DRAFT_0165 at 112.809 kb on + strand, within EX28DRAFT_0165 at 112.926 kb on - strand, within EX28DRAFT_0165 at 113.030 kb on - strand, within EX28DRAFT_0165 at 113.259 kb on + strand, within EX28DRAFT_0165 at 113.589 kb on - strand, within EX28DRAFT_0165 at 113.770 kb on + strand at 113.786 kb on - strand at 113.823 kb on + strand at 113.898 kb on + strand at 113.899 kb on - strand at 113.899 kb on - strand at 113.899 kb on - strand at 114.036 kb on + strand, within EX28DRAFT_0166 at 114.036 kb on + strand, within EX28DRAFT_0166 at 114.037 kb on - strand, within EX28DRAFT_0166 at 114.037 kb on - strand, within EX28DRAFT_0166 at 114.046 kb on + strand, within EX28DRAFT_0166 at 114.067 kb on + strand, within EX28DRAFT_0166 at 114.068 kb on - strand, within EX28DRAFT_0166 at 114.208 kb on - strand, within EX28DRAFT_0166 at 114.208 kb on - strand, within EX28DRAFT_0166 at 114.313 kb on - strand, within EX28DRAFT_0166 at 114.453 kb on + strand, within EX28DRAFT_0166 at 114.454 kb on - strand, within EX28DRAFT_0166 at 114.735 kb on + strand, within EX28DRAFT_0166
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 111,334 + EX28DRAFT_0164 0.59 +0.2 111,335 - EX28DRAFT_0164 0.59 +0.5 111,335 - EX28DRAFT_0164 0.59 +0.3 111,335 - EX28DRAFT_0164 0.59 +0.5 111,335 - EX28DRAFT_0164 0.59 -0.0 111,340 + EX28DRAFT_0164 0.60 +0.4 111,340 + EX28DRAFT_0164 0.60 +0.3 111,341 - EX28DRAFT_0164 0.60 +0.5 111,341 - EX28DRAFT_0164 0.60 -0.2 111,367 + EX28DRAFT_0164 0.61 +0.2 111,602 + EX28DRAFT_0164 0.71 +0.3 111,817 + EX28DRAFT_0164 0.81 +0.1 111,818 - EX28DRAFT_0164 0.81 -0.4 111,891 - EX28DRAFT_0164 0.84 -0.6 111,927 + EX28DRAFT_0164 0.86 -0.3 111,928 - EX28DRAFT_0164 0.86 -1.7 111,928 - EX28DRAFT_0164 0.86 -1.0 111,964 + EX28DRAFT_0164 0.88 +0.8 111,964 + EX28DRAFT_0164 0.88 +0.1 111,965 - EX28DRAFT_0164 0.88 -0.0 112,112 - +0.4 112,130 + -0.1 112,130 + -0.3 112,131 - -0.3 112,131 - -0.2 112,135 - +0.3 112,162 + +0.4 112,162 + -0.1 112,163 - -0.9 112,627 - EX28DRAFT_0165 0.25 -1.0 112,755 + EX28DRAFT_0165 0.33 +1.2 112,756 - EX28DRAFT_0165 0.33 -0.1 112,809 + EX28DRAFT_0165 0.37 -0.0 112,926 - EX28DRAFT_0165 0.44 -1.3 113,030 - EX28DRAFT_0165 0.51 -1.1 113,259 + EX28DRAFT_0165 0.66 -0.8 113,589 - EX28DRAFT_0165 0.87 -1.9 113,770 + -0.9 113,786 - -0.2 113,823 + -0.6 113,898 + -1.9 113,899 - -0.2 113,899 - -0.4 113,899 - -0.1 114,036 + EX28DRAFT_0166 0.12 -0.5 114,036 + EX28DRAFT_0166 0.12 +0.5 114,037 - EX28DRAFT_0166 0.12 +0.1 114,037 - EX28DRAFT_0166 0.12 -1.1 114,046 + EX28DRAFT_0166 0.12 -1.0 114,067 + EX28DRAFT_0166 0.13 -0.3 114,068 - EX28DRAFT_0166 0.14 -1.7 114,208 - EX28DRAFT_0166 0.20 +0.1 114,208 - EX28DRAFT_0166 0.20 +1.3 114,313 - EX28DRAFT_0166 0.25 +1.9 114,453 + EX28DRAFT_0166 0.32 -2.8 114,454 - EX28DRAFT_0166 0.32 -1.3 114,735 + EX28DRAFT_0166 0.46 -1.2
Or see this region's nucleotide sequence