Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0032 and EX28DRAFT_0033 are separated by 40 nucleotides EX28DRAFT_0033 and EX28DRAFT_0034 are separated by 107 nucleotides EX28DRAFT_0034 and EX28DRAFT_0035 are separated by 2 nucleotides EX28DRAFT_0035 and EX28DRAFT_0036 are separated by 57 nucleotides EX28DRAFT_0036 and EX28DRAFT_0037 are separated by 0 nucleotides
EX28DRAFT_0032: EX28DRAFT_0032 - DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6), at 14,505 to 15,494
_0032
EX28DRAFT_0033: EX28DRAFT_0033 - LSU ribosomal protein L17P, at 15,535 to 15,918
_0033
EX28DRAFT_0034: EX28DRAFT_0034 - Domain of unknown function (DUF1992), at 16,026 to 16,394
_0034
EX28DRAFT_0035: EX28DRAFT_0035 - Zn(II)-responsive transcriptional regulator, at 16,397 to 16,822
_0035
EX28DRAFT_0036: EX28DRAFT_0036 - Uncharacterized protein conserved in bacteria, at 16,880 to 17,095
_0036
EX28DRAFT_0037: EX28DRAFT_0037 - large conductance mechanosensitive channel protein, at 17,096 to 17,506
_0037
Position (kb)
16
17 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 15.960 kb on - strand at 15.985 kb on - strand at 15.985 kb on - strand at 15.996 kb on - strand at 15.999 kb on - strand at 16.059 kb on - strand at 16.153 kb on + strand, within EX28DRAFT_0034 at 16.316 kb on - strand, within EX28DRAFT_0034 at 16.393 kb on - strand at 16.396 kb on + strand at 16.396 kb on + strand at 16.397 kb on - strand at 16.397 kb on - strand at 16.397 kb on - strand at 16.397 kb on - strand at 16.400 kb on + strand at 16.400 kb on + strand at 16.455 kb on - strand, within EX28DRAFT_0035 at 16.492 kb on + strand, within EX28DRAFT_0035 at 16.493 kb on - strand, within EX28DRAFT_0035 at 16.514 kb on + strand, within EX28DRAFT_0035 at 16.515 kb on - strand, within EX28DRAFT_0035 at 16.596 kb on + strand, within EX28DRAFT_0035 at 16.597 kb on - strand, within EX28DRAFT_0035 at 16.643 kb on - strand, within EX28DRAFT_0035 at 16.643 kb on - strand, within EX28DRAFT_0035 at 16.684 kb on + strand, within EX28DRAFT_0035 at 16.685 kb on - strand, within EX28DRAFT_0035 at 16.687 kb on - strand, within EX28DRAFT_0035 at 16.828 kb on - strand at 16.880 kb on - strand at 16.912 kb on + strand, within EX28DRAFT_0036 at 16.935 kb on + strand, within EX28DRAFT_0036 at 16.935 kb on + strand, within EX28DRAFT_0036 at 17.026 kb on + strand, within EX28DRAFT_0036 at 17.061 kb on + strand, within EX28DRAFT_0036 at 17.086 kb on + strand at 17.086 kb on + strand at 17.087 kb on - strand at 17.091 kb on - strand at 17.228 kb on - strand, within EX28DRAFT_0037 at 17.228 kb on - strand, within EX28DRAFT_0037 at 17.228 kb on - strand, within EX28DRAFT_0037 at 17.262 kb on - strand, within EX28DRAFT_0037 at 17.291 kb on + strand, within EX28DRAFT_0037 at 17.291 kb on + strand, within EX28DRAFT_0037 at 17.294 kb on + strand, within EX28DRAFT_0037 at 17.295 kb on - strand, within EX28DRAFT_0037 at 17.386 kb on + strand, within EX28DRAFT_0037
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 15,960 - +0.7 15,985 - +1.6 15,985 - +0.1 15,996 - -0.3 15,999 - -1.3 16,059 - +0.6 16,153 + EX28DRAFT_0034 0.34 +0.6 16,316 - EX28DRAFT_0034 0.79 +0.2 16,393 - +0.4 16,396 + +0.6 16,396 + -2.6 16,397 - +0.4 16,397 - -0.4 16,397 - +0.4 16,397 - -1.1 16,400 + +0.3 16,400 + +0.1 16,455 - EX28DRAFT_0035 0.14 -1.2 16,492 + EX28DRAFT_0035 0.22 +0.1 16,493 - EX28DRAFT_0035 0.23 +0.8 16,514 + EX28DRAFT_0035 0.27 +0.2 16,515 - EX28DRAFT_0035 0.28 +0.0 16,596 + EX28DRAFT_0035 0.47 -0.3 16,597 - EX28DRAFT_0035 0.47 -0.0 16,643 - EX28DRAFT_0035 0.58 -2.2 16,643 - EX28DRAFT_0035 0.58 +0.2 16,684 + EX28DRAFT_0035 0.67 +0.8 16,685 - EX28DRAFT_0035 0.68 +0.0 16,687 - EX28DRAFT_0035 0.68 +1.8 16,828 - -0.7 16,880 - +0.4 16,912 + EX28DRAFT_0036 0.15 +0.1 16,935 + EX28DRAFT_0036 0.25 -0.1 16,935 + EX28DRAFT_0036 0.25 +0.1 17,026 + EX28DRAFT_0036 0.68 -0.0 17,061 + EX28DRAFT_0036 0.84 +0.2 17,086 + -0.2 17,086 + +0.6 17,087 - +0.6 17,091 - +0.0 17,228 - EX28DRAFT_0037 0.32 -1.2 17,228 - EX28DRAFT_0037 0.32 +0.9 17,228 - EX28DRAFT_0037 0.32 +0.4 17,262 - EX28DRAFT_0037 0.40 +0.7 17,291 + EX28DRAFT_0037 0.47 +0.2 17,291 + EX28DRAFT_0037 0.47 -0.4 17,294 + EX28DRAFT_0037 0.48 +0.8 17,295 - EX28DRAFT_0037 0.48 +1.1 17,386 + EX28DRAFT_0037 0.71 +0.1
Or see this region's nucleotide sequence