Strain Fitness in Escherichia coli BL21 around ECD_02192

Experiment: NophageControl

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02191 and ECD_02192 are separated by 88 nucleotidesECD_02192 and ECD_02193 are separated by 78 nucleotidesECD_02193 and ECD_02194 are separated by 54 nucleotidesECD_02194 and ECD_02195 are separated by 64 nucleotides ECD_02191: ECD_02191 - 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylate synthase; SEPHCHC synthase, at 2,267,763 to 2,269,433 _02191 ECD_02192: ECD_02192 - isochorismate synthase 2, at 2,269,522 to 2,270,817 _02192 ECD_02193: ECD_02193 - putative membrane-anchored DUF883 family ribosome-binding protein, at 2,270,896 to 2,271,201 _02193 ECD_02194: ECD_02194 - GNAT family putative N-acetyltransferase, at 2,271,256 to 2,271,717 _02194 ECD_02195: ECD_02195 - RNase BN, tRNA processing enzyme, at 2,271,782 to 2,272,699 _02195 Position (kb) 2269 2270 2271Strain fitness (log2 ratio) -1 0 1 2 3at 2268.884 kb on + strand, within ECD_02191at 2268.885 kb on - strand, within ECD_02191at 2268.885 kb on - strand, within ECD_02191at 2268.911 kb on - strand, within ECD_02191at 2269.102 kb on - strand, within ECD_02191at 2269.331 kb on + strandat 2269.416 kb on - strandat 2269.426 kb on - strandat 2269.426 kb on - strandat 2269.461 kb on + strandat 2269.466 kb on - strandat 2269.468 kb on - strandat 2269.468 kb on - strandat 2269.484 kb on - strandat 2269.624 kb on + strandat 2269.624 kb on + strandat 2269.624 kb on + strandat 2269.811 kb on - strand, within ECD_02192at 2269.931 kb on - strand, within ECD_02192at 2270.006 kb on - strand, within ECD_02192at 2270.035 kb on - strand, within ECD_02192at 2270.498 kb on - strand, within ECD_02192at 2270.644 kb on - strand, within ECD_02192at 2270.806 kb on - strandat 2270.921 kb on - strandat 2270.979 kb on - strand, within ECD_02193at 2270.979 kb on - strand, within ECD_02193at 2271.015 kb on - strand, within ECD_02193at 2271.018 kb on - strand, within ECD_02193at 2271.018 kb on - strand, within ECD_02193at 2271.018 kb on - strand, within ECD_02193at 2271.018 kb on - strand, within ECD_02193at 2271.018 kb on - strand, within ECD_02193at 2271.095 kb on + strand, within ECD_02193at 2271.180 kb on + strandat 2271.234 kb on - strandat 2271.237 kb on + strandat 2271.238 kb on - strandat 2271.238 kb on - strandat 2271.311 kb on + strand, within ECD_02194at 2271.347 kb on + strand, within ECD_02194at 2271.409 kb on - strand, within ECD_02194at 2271.666 kb on - strand, within ECD_02194at 2271.718 kb on - strandat 2271.737 kb on + strandat 2271.737 kb on + strandat 2271.738 kb on - strandat 2271.738 kb on - strandat 2271.738 kb on - strandat 2271.745 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NophageControl
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2,268,884 + ECD_02191 0.67 -0.4
2,268,885 - ECD_02191 0.67 -0.4
2,268,885 - ECD_02191 0.67 -1.2
2,268,911 - ECD_02191 0.69 +0.4
2,269,102 - ECD_02191 0.80 -0.7
2,269,331 + +2.1
2,269,416 - +0.7
2,269,426 - +3.5
2,269,426 - +0.0
2,269,461 + +0.6
2,269,466 - -0.8
2,269,468 - +0.4
2,269,468 - +0.7
2,269,484 - +0.5
2,269,624 + -0.0
2,269,624 + +1.2
2,269,624 + +0.7
2,269,811 - ECD_02192 0.22 +1.5
2,269,931 - ECD_02192 0.32 -0.2
2,270,006 - ECD_02192 0.37 -1.0
2,270,035 - ECD_02192 0.40 -1.1
2,270,498 - ECD_02192 0.75 +0.7
2,270,644 - ECD_02192 0.87 -0.7
2,270,806 - -0.0
2,270,921 - +1.0
2,270,979 - ECD_02193 0.27 +0.2
2,270,979 - ECD_02193 0.27 +0.7
2,271,015 - ECD_02193 0.39 -0.0
2,271,018 - ECD_02193 0.40 +0.1
2,271,018 - ECD_02193 0.40 -1.1
2,271,018 - ECD_02193 0.40 +1.0
2,271,018 - ECD_02193 0.40 +1.1
2,271,018 - ECD_02193 0.40 +0.6
2,271,095 + ECD_02193 0.65 +0.9
2,271,180 + -0.3
2,271,234 - +0.3
2,271,237 + +0.9
2,271,238 - -0.1
2,271,238 - +0.3
2,271,311 + ECD_02194 0.12 -1.2
2,271,347 + ECD_02194 0.20 +0.1
2,271,409 - ECD_02194 0.33 -0.3
2,271,666 - ECD_02194 0.89 -0.0
2,271,718 - -0.1
2,271,737 + -0.3
2,271,737 + +0.0
2,271,738 - -0.8
2,271,738 - -1.3
2,271,738 - -0.1
2,271,745 - -0.1

Or see this region's nucleotide sequence