Experiment: NophageControl
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01131 and ECD_01132 are separated by 53 nucleotides ECD_01132 and ECD_01133 are separated by 9 nucleotides ECD_01133 and ECD_01134 are separated by 171 nucleotides
ECD_01131: ECD_01131 - tRNA(Gln,Lys,Glu) U34 2-thiouridylase, at 1,196,034 to 1,197,140
_01131
ECD_01132: ECD_01132 - bifunctional thiamine pyrimidine pyrophosphate hydrolase and thiamine pyrophosphate hydrolase, at 1,197,194 to 1,197,655
_01132
ECD_01133: ECD_01133 - 23S rRNA pseudouridine(2457) synthase, at 1,197,665 to 1,198,318
_01133
ECD_01134: ECD_01134 - isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase, at 1,198,490 to 1,199,740
_01134
Position (kb)
1197
1198
1199 Strain fitness (log2 ratio)
-1
0
1
2 at 1197.212 kb on - strand at 1197.224 kb on - strand at 1197.581 kb on - strand, within ECD_01132 at 1197.715 kb on - strand at 1197.801 kb on + strand, within ECD_01133 at 1197.802 kb on - strand, within ECD_01133 at 1197.802 kb on - strand, within ECD_01133 at 1197.802 kb on - strand, within ECD_01133 at 1197.804 kb on - strand, within ECD_01133 at 1197.804 kb on - strand, within ECD_01133 at 1197.804 kb on - strand, within ECD_01133 at 1197.804 kb on - strand, within ECD_01133 at 1197.804 kb on - strand, within ECD_01133 at 1197.804 kb on - strand, within ECD_01133 at 1198.177 kb on - strand, within ECD_01133 at 1198.321 kb on + strand at 1198.322 kb on - strand at 1198.381 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NophageControl remove 1,197,212 - -0.9 1,197,224 - +1.0 1,197,581 - ECD_01132 0.84 -0.5 1,197,715 - +0.6 1,197,801 + ECD_01133 0.21 +2.1 1,197,802 - ECD_01133 0.21 +0.8 1,197,802 - ECD_01133 0.21 -1.4 1,197,802 - ECD_01133 0.21 +1.2 1,197,804 - ECD_01133 0.21 +0.1 1,197,804 - ECD_01133 0.21 -1.6 1,197,804 - ECD_01133 0.21 +1.1 1,197,804 - ECD_01133 0.21 +0.0 1,197,804 - ECD_01133 0.21 +0.7 1,197,804 - ECD_01133 0.21 +0.8 1,198,177 - ECD_01133 0.78 +0.1 1,198,321 + +1.4 1,198,322 - +0.2 1,198,381 + +0.5
Or see this region's nucleotide sequence