Experiment: L-Lysine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS12175 and ABIE40_RS12180 are separated by 28 nucleotides ABIE40_RS12180 and ABIE40_RS12185 are separated by 54 nucleotides ABIE40_RS12185 and ABIE40_RS12190 are separated by 384 nucleotides ABIE40_RS12190 and ABIE40_RS12195 are separated by 78 nucleotides
ABIE40_RS12175: ABIE40_RS12175 - DUF983 domain-containing protein, at 2,374,847 to 2,375,233
_RS12175
ABIE40_RS12180: ABIE40_RS12180 - 4-oxalocrotonate tautomerase family protein, at 2,375,262 to 2,375,570
_RS12180
ABIE40_RS12185: ABIE40_RS12185 - zinc-dependent alcohol dehydrogenase family protein, at 2,375,625 to 2,376,614
_RS12185
ABIE40_RS12190: ABIE40_RS12190 - hypothetical protein, at 2,376,999 to 2,377,532
_RS12190
ABIE40_RS12195: ABIE40_RS12195 - PLP-dependent aspartate aminotransferase family protein, at 2,377,611 to 2,378,792
_RS12195
Position (kb)
2375
2376
2377 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2374.637 kb on + strand at 2374.637 kb on + strand at 2374.637 kb on + strand at 2374.637 kb on + strand at 2374.637 kb on + strand at 2374.637 kb on + strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.638 kb on - strand at 2374.639 kb on + strand at 2374.796 kb on - strand at 2374.796 kb on - strand at 2374.796 kb on - strand at 2374.796 kb on - strand at 2374.796 kb on - strand at 2374.796 kb on - strand at 2374.799 kb on + strand at 2374.799 kb on + strand at 2374.799 kb on + strand at 2374.799 kb on + strand at 2374.799 kb on + strand at 2374.800 kb on - strand at 2374.800 kb on - strand at 2374.800 kb on - strand at 2374.800 kb on - strand at 2374.800 kb on - strand at 2374.849 kb on - strand at 2374.849 kb on - strand at 2374.850 kb on + strand at 2374.851 kb on - strand at 2374.864 kb on + strand at 2374.864 kb on + strand at 2374.864 kb on + strand at 2374.864 kb on + strand at 2374.865 kb on - strand at 2374.865 kb on - strand at 2374.868 kb on + strand at 2374.868 kb on + strand at 2374.868 kb on + strand at 2374.869 kb on - strand at 2374.890 kb on + strand, within ABIE40_RS12175 at 2374.890 kb on + strand, within ABIE40_RS12175 at 2374.890 kb on + strand, within ABIE40_RS12175 at 2374.891 kb on - strand, within ABIE40_RS12175 at 2374.891 kb on - strand, within ABIE40_RS12175 at 2374.896 kb on + strand, within ABIE40_RS12175 at 2374.896 kb on + strand, within ABIE40_RS12175 at 2374.897 kb on - strand, within ABIE40_RS12175 at 2374.897 kb on - strand, within ABIE40_RS12175 at 2375.194 kb on + strand, within ABIE40_RS12175 at 2375.194 kb on + strand, within ABIE40_RS12175 at 2375.195 kb on - strand at 2375.195 kb on - strand at 2375.195 kb on - strand at 2375.195 kb on - strand at 2375.195 kb on - strand at 2375.195 kb on - strand at 2375.221 kb on - strand at 2375.221 kb on - strand at 2375.221 kb on - strand at 2375.247 kb on + strand at 2375.248 kb on - strand at 2375.248 kb on - strand at 2375.248 kb on - strand at 2375.363 kb on + strand, within ABIE40_RS12180 at 2375.364 kb on - strand, within ABIE40_RS12180 at 2375.364 kb on - strand, within ABIE40_RS12180 at 2375.420 kb on + strand, within ABIE40_RS12180 at 2375.421 kb on - strand, within ABIE40_RS12180 at 2375.421 kb on - strand, within ABIE40_RS12180 at 2375.456 kb on + strand, within ABIE40_RS12180 at 2375.456 kb on + strand, within ABIE40_RS12180 at 2375.457 kb on - strand, within ABIE40_RS12180 at 2375.457 kb on - strand, within ABIE40_RS12180 at 2375.457 kb on - strand, within ABIE40_RS12180 at 2375.457 kb on - strand, within ABIE40_RS12180 at 2375.496 kb on - strand, within ABIE40_RS12180 at 2375.526 kb on - strand, within ABIE40_RS12180 at 2375.594 kb on + strand at 2375.594 kb on + strand at 2375.594 kb on + strand at 2375.595 kb on - strand at 2375.595 kb on - strand at 2375.595 kb on - strand at 2375.595 kb on - strand at 2375.595 kb on - strand at 2375.836 kb on + strand, within ABIE40_RS12185 at 2375.837 kb on - strand, within ABIE40_RS12185 at 2375.865 kb on + strand, within ABIE40_RS12185 at 2375.866 kb on - strand, within ABIE40_RS12185 at 2375.866 kb on - strand, within ABIE40_RS12185 at 2376.000 kb on + strand, within ABIE40_RS12185 at 2376.000 kb on + strand, within ABIE40_RS12185 at 2376.000 kb on + strand, within ABIE40_RS12185 at 2376.001 kb on - strand, within ABIE40_RS12185 at 2376.001 kb on - strand, within ABIE40_RS12185 at 2376.004 kb on + strand, within ABIE40_RS12185 at 2376.004 kb on + strand, within ABIE40_RS12185 at 2376.004 kb on + strand, within ABIE40_RS12185 at 2376.004 kb on + strand, within ABIE40_RS12185 at 2376.005 kb on - strand, within ABIE40_RS12185 at 2376.005 kb on - strand, within ABIE40_RS12185 at 2376.061 kb on + strand, within ABIE40_RS12185 at 2376.061 kb on + strand, within ABIE40_RS12185 at 2376.091 kb on + strand, within ABIE40_RS12185 at 2376.091 kb on + strand, within ABIE40_RS12185 at 2376.131 kb on + strand, within ABIE40_RS12185 at 2376.131 kb on + strand, within ABIE40_RS12185 at 2376.132 kb on - strand, within ABIE40_RS12185 at 2376.132 kb on - strand, within ABIE40_RS12185 at 2376.132 kb on - strand, within ABIE40_RS12185 at 2376.132 kb on - strand, within ABIE40_RS12185 at 2376.132 kb on - strand, within ABIE40_RS12185 at 2376.257 kb on + strand, within ABIE40_RS12185 at 2376.257 kb on + strand, within ABIE40_RS12185 at 2376.257 kb on + strand, within ABIE40_RS12185 at 2376.257 kb on + strand, within ABIE40_RS12185 at 2376.258 kb on - strand, within ABIE40_RS12185 at 2376.258 kb on - strand, within ABIE40_RS12185 at 2376.258 kb on - strand, within ABIE40_RS12185 at 2376.258 kb on - strand, within ABIE40_RS12185 at 2376.258 kb on - strand, within ABIE40_RS12185 at 2376.258 kb on - strand, within ABIE40_RS12185 at 2376.305 kb on - strand, within ABIE40_RS12185 at 2376.305 kb on - strand, within ABIE40_RS12185 at 2376.305 kb on - strand, within ABIE40_RS12185 at 2376.483 kb on - strand, within ABIE40_RS12185 at 2376.561 kb on + strand at 2376.564 kb on + strand at 2376.564 kb on + strand at 2376.565 kb on - strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.676 kb on + strand at 2376.677 kb on - strand at 2376.677 kb on - strand at 2376.677 kb on - strand at 2376.677 kb on - strand at 2376.677 kb on - strand at 2376.678 kb on + strand at 2376.678 kb on + strand at 2376.678 kb on + strand at 2376.678 kb on + strand at 2376.678 kb on + strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.679 kb on - strand at 2376.680 kb on + strand at 2376.681 kb on - strand at 2376.681 kb on - strand at 2376.754 kb on + strand at 2376.755 kb on - strand at 2376.755 kb on - strand at 2376.786 kb on + strand at 2376.786 kb on + strand at 2376.787 kb on - strand at 2376.787 kb on - strand at 2376.787 kb on - strand at 2376.787 kb on - strand at 2376.787 kb on - strand at 2376.787 kb on - strand at 2376.899 kb on + strand at 2376.899 kb on + strand at 2376.902 kb on + strand at 2376.903 kb on - strand at 2376.934 kb on + strand at 2376.934 kb on + strand at 2376.935 kb on - strand at 2376.935 kb on - strand at 2376.935 kb on - strand at 2376.935 kb on - strand at 2376.935 kb on - strand at 2376.992 kb on + strand at 2376.993 kb on - strand at 2377.091 kb on - strand, within ABIE40_RS12190 at 2377.213 kb on + strand, within ABIE40_RS12190 at 2377.213 kb on + strand, within ABIE40_RS12190 at 2377.214 kb on - strand, within ABIE40_RS12190 at 2377.219 kb on + strand, within ABIE40_RS12190 at 2377.219 kb on + strand, within ABIE40_RS12190 at 2377.219 kb on + strand, within ABIE40_RS12190 at 2377.220 kb on - strand, within ABIE40_RS12190 at 2377.220 kb on - strand, within ABIE40_RS12190 at 2377.267 kb on + strand, within ABIE40_RS12190 at 2377.342 kb on + strand, within ABIE40_RS12190 at 2377.343 kb on - strand, within ABIE40_RS12190 at 2377.343 kb on - strand, within ABIE40_RS12190 at 2377.343 kb on - strand, within ABIE40_RS12190 at 2377.356 kb on + strand, within ABIE40_RS12190 at 2377.356 kb on + strand, within ABIE40_RS12190 at 2377.356 kb on + strand, within ABIE40_RS12190 at 2377.356 kb on + strand, within ABIE40_RS12190 at 2377.356 kb on + strand, within ABIE40_RS12190 at 2377.357 kb on - strand, within ABIE40_RS12190 at 2377.357 kb on - strand, within ABIE40_RS12190 at 2377.363 kb on + strand, within ABIE40_RS12190 at 2377.363 kb on + strand, within ABIE40_RS12190 at 2377.363 kb on + strand, within ABIE40_RS12190 at 2377.363 kb on + strand, within ABIE40_RS12190 at 2377.363 kb on + strand, within ABIE40_RS12190 at 2377.364 kb on - strand, within ABIE40_RS12190 at 2377.364 kb on - strand, within ABIE40_RS12190 at 2377.364 kb on - strand, within ABIE40_RS12190 at 2377.364 kb on - strand, within ABIE40_RS12190 at 2377.407 kb on + strand, within ABIE40_RS12190 at 2377.407 kb on + strand, within ABIE40_RS12190 at 2377.407 kb on + strand, within ABIE40_RS12190 at 2377.407 kb on + strand, within ABIE40_RS12190 at 2377.408 kb on - strand, within ABIE40_RS12190 at 2377.408 kb on - strand, within ABIE40_RS12190 at 2377.408 kb on - strand, within ABIE40_RS12190 at 2377.498 kb on + strand at 2377.498 kb on + strand at 2377.498 kb on + strand at 2377.498 kb on + strand at 2377.499 kb on - strand at 2377.499 kb on - strand at 2377.499 kb on - strand at 2377.530 kb on + strand at 2377.530 kb on + strand at 2377.531 kb on - strand at 2377.562 kb on + strand at 2377.562 kb on + strand at 2377.562 kb on + strand at 2377.562 kb on + strand at 2377.563 kb on - strand at 2377.563 kb on - strand at 2377.563 kb on - strand at 2377.565 kb on + strand at 2377.565 kb on + strand at 2377.566 kb on - strand at 2377.581 kb on - strand at 2377.581 kb on - strand at 2377.581 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (C) remove 2,374,637 + +0.8 2,374,637 + -0.2 2,374,637 + -0.9 2,374,637 + -0.7 2,374,637 + -1.1 2,374,637 + +1.2 2,374,638 - +2.8 2,374,638 - -0.3 2,374,638 - -0.5 2,374,638 - -1.7 2,374,638 - -0.8 2,374,638 - +0.2 2,374,638 - -0.1 2,374,638 - +0.5 2,374,639 + +1.5 2,374,796 - +0.2 2,374,796 - -3.7 2,374,796 - +0.0 2,374,796 - -1.0 2,374,796 - -0.6 2,374,796 - -0.5 2,374,799 + -1.4 2,374,799 + +0.4 2,374,799 + -0.2 2,374,799 + -0.6 2,374,799 + +1.7 2,374,800 - -0.7 2,374,800 - +0.1 2,374,800 - -0.3 2,374,800 - -1.7 2,374,800 - +0.1 2,374,849 - -0.8 2,374,849 - +0.6 2,374,850 + +0.4 2,374,851 - +0.5 2,374,864 + -0.2 2,374,864 + -0.2 2,374,864 + +1.1 2,374,864 + +0.2 2,374,865 - -0.5 2,374,865 - -1.9 2,374,868 + -0.7 2,374,868 + +0.2 2,374,868 + +1.1 2,374,869 - +0.7 2,374,890 + ABIE40_RS12175 0.11 -1.0 2,374,890 + ABIE40_RS12175 0.11 +1.2 2,374,890 + ABIE40_RS12175 0.11 +1.4 2,374,891 - ABIE40_RS12175 0.11 +1.0 2,374,891 - ABIE40_RS12175 0.11 -0.1 2,374,896 + ABIE40_RS12175 0.13 +0.0 2,374,896 + ABIE40_RS12175 0.13 +0.2 2,374,897 - ABIE40_RS12175 0.13 -0.6 2,374,897 - ABIE40_RS12175 0.13 -0.1 2,375,194 + ABIE40_RS12175 0.90 -1.5 2,375,194 + ABIE40_RS12175 0.90 +1.3 2,375,195 - -0.9 2,375,195 - -1.0 2,375,195 - -0.5 2,375,195 - +0.6 2,375,195 - +1.0 2,375,195 - -2.6 2,375,221 - +0.3 2,375,221 - +0.7 2,375,221 - -0.2 2,375,247 + +0.9 2,375,248 - -0.6 2,375,248 - +1.5 2,375,248 - +0.2 2,375,363 + ABIE40_RS12180 0.33 +0.0 2,375,364 - ABIE40_RS12180 0.33 -1.7 2,375,364 - ABIE40_RS12180 0.33 -1.7 2,375,420 + ABIE40_RS12180 0.51 -1.4 2,375,421 - ABIE40_RS12180 0.51 +2.3 2,375,421 - ABIE40_RS12180 0.51 -0.1 2,375,456 + ABIE40_RS12180 0.63 -1.4 2,375,456 + ABIE40_RS12180 0.63 +0.8 2,375,457 - ABIE40_RS12180 0.63 -0.3 2,375,457 - ABIE40_RS12180 0.63 +0.5 2,375,457 - ABIE40_RS12180 0.63 +0.1 2,375,457 - ABIE40_RS12180 0.63 -0.1 2,375,496 - ABIE40_RS12180 0.76 -0.3 2,375,526 - ABIE40_RS12180 0.85 -0.0 2,375,594 + -0.2 2,375,594 + -0.9 2,375,594 + +0.2 2,375,595 - -0.1 2,375,595 - -1.0 2,375,595 - +0.0 2,375,595 - +0.5 2,375,595 - -0.7 2,375,836 + ABIE40_RS12185 0.21 -1.9 2,375,837 - ABIE40_RS12185 0.21 +0.2 2,375,865 + ABIE40_RS12185 0.24 +0.1 2,375,866 - ABIE40_RS12185 0.24 +0.9 2,375,866 - ABIE40_RS12185 0.24 -0.3 2,376,000 + ABIE40_RS12185 0.38 -0.3 2,376,000 + ABIE40_RS12185 0.38 -0.5 2,376,000 + ABIE40_RS12185 0.38 +0.5 2,376,001 - ABIE40_RS12185 0.38 +0.1 2,376,001 - ABIE40_RS12185 0.38 -0.2 2,376,004 + ABIE40_RS12185 0.38 -0.9 2,376,004 + ABIE40_RS12185 0.38 +3.3 2,376,004 + ABIE40_RS12185 0.38 -0.7 2,376,004 + ABIE40_RS12185 0.38 -0.3 2,376,005 - ABIE40_RS12185 0.38 +1.3 2,376,005 - ABIE40_RS12185 0.38 +0.1 2,376,061 + ABIE40_RS12185 0.44 +0.2 2,376,061 + ABIE40_RS12185 0.44 -0.4 2,376,091 + ABIE40_RS12185 0.47 +1.8 2,376,091 + ABIE40_RS12185 0.47 -0.9 2,376,131 + ABIE40_RS12185 0.51 -1.7 2,376,131 + ABIE40_RS12185 0.51 +0.5 2,376,132 - ABIE40_RS12185 0.51 +0.8 2,376,132 - ABIE40_RS12185 0.51 -1.9 2,376,132 - ABIE40_RS12185 0.51 -0.3 2,376,132 - ABIE40_RS12185 0.51 -0.5 2,376,132 - ABIE40_RS12185 0.51 +1.1 2,376,257 + ABIE40_RS12185 0.64 -0.2 2,376,257 + ABIE40_RS12185 0.64 +1.8 2,376,257 + ABIE40_RS12185 0.64 -0.9 2,376,257 + ABIE40_RS12185 0.64 +0.3 2,376,258 - ABIE40_RS12185 0.64 +1.2 2,376,258 - ABIE40_RS12185 0.64 +0.7 2,376,258 - ABIE40_RS12185 0.64 +2.2 2,376,258 - ABIE40_RS12185 0.64 +0.3 2,376,258 - ABIE40_RS12185 0.64 +0.2 2,376,258 - ABIE40_RS12185 0.64 +1.1 2,376,305 - ABIE40_RS12185 0.69 +1.3 2,376,305 - ABIE40_RS12185 0.69 -0.0 2,376,305 - ABIE40_RS12185 0.69 +0.0 2,376,483 - ABIE40_RS12185 0.87 +1.7 2,376,561 + +0.1 2,376,564 + +0.7 2,376,564 + -0.3 2,376,565 - -0.9 2,376,676 + +1.0 2,376,676 + -0.3 2,376,676 + -0.5 2,376,676 + -0.3 2,376,676 + -0.1 2,376,676 + +0.3 2,376,676 + -0.7 2,376,676 + +1.1 2,376,676 + -0.4 2,376,676 + +0.5 2,376,676 + -0.3 2,376,676 + -0.0 2,376,676 + -0.3 2,376,677 - +0.7 2,376,677 - +0.3 2,376,677 - +0.0 2,376,677 - +0.5 2,376,677 - +1.9 2,376,678 + -0.3 2,376,678 + -1.5 2,376,678 + +0.3 2,376,678 + -0.3 2,376,678 + +0.0 2,376,679 - -0.4 2,376,679 - +1.1 2,376,679 - -0.2 2,376,679 - +1.3 2,376,679 - +0.1 2,376,679 - -0.6 2,376,679 - -0.4 2,376,679 - +0.1 2,376,679 - -1.9 2,376,679 - -0.5 2,376,680 + +0.6 2,376,681 - -1.6 2,376,681 - -0.3 2,376,754 + -0.7 2,376,755 - -2.6 2,376,755 - -0.1 2,376,786 + -0.2 2,376,786 + -0.7 2,376,787 - +1.2 2,376,787 - -0.4 2,376,787 - -0.5 2,376,787 - -0.4 2,376,787 - -0.2 2,376,787 - +0.2 2,376,899 + +0.9 2,376,899 + -0.6 2,376,902 + -0.4 2,376,903 - -0.0 2,376,934 + -0.9 2,376,934 + +0.0 2,376,935 - -0.6 2,376,935 - +0.6 2,376,935 - -1.1 2,376,935 - +1.2 2,376,935 - -1.2 2,376,992 + -0.4 2,376,993 - -0.9 2,377,091 - ABIE40_RS12190 0.17 -1.1 2,377,213 + ABIE40_RS12190 0.40 -1.5 2,377,213 + ABIE40_RS12190 0.40 +0.4 2,377,214 - ABIE40_RS12190 0.40 +0.1 2,377,219 + ABIE40_RS12190 0.41 -1.0 2,377,219 + ABIE40_RS12190 0.41 +0.5 2,377,219 + ABIE40_RS12190 0.41 -0.0 2,377,220 - ABIE40_RS12190 0.41 -0.4 2,377,220 - ABIE40_RS12190 0.41 +0.5 2,377,267 + ABIE40_RS12190 0.50 +0.1 2,377,342 + ABIE40_RS12190 0.64 +2.9 2,377,343 - ABIE40_RS12190 0.64 -0.1 2,377,343 - ABIE40_RS12190 0.64 +0.4 2,377,343 - ABIE40_RS12190 0.64 -0.8 2,377,356 + ABIE40_RS12190 0.67 +0.7 2,377,356 + ABIE40_RS12190 0.67 -2.3 2,377,356 + ABIE40_RS12190 0.67 -0.1 2,377,356 + ABIE40_RS12190 0.67 -0.1 2,377,356 + ABIE40_RS12190 0.67 +2.2 2,377,357 - ABIE40_RS12190 0.67 -0.2 2,377,357 - ABIE40_RS12190 0.67 +0.4 2,377,363 + ABIE40_RS12190 0.68 -0.7 2,377,363 + ABIE40_RS12190 0.68 -0.2 2,377,363 + ABIE40_RS12190 0.68 -0.6 2,377,363 + ABIE40_RS12190 0.68 -0.4 2,377,363 + ABIE40_RS12190 0.68 -1.3 2,377,364 - ABIE40_RS12190 0.68 -1.9 2,377,364 - ABIE40_RS12190 0.68 -0.3 2,377,364 - ABIE40_RS12190 0.68 -1.5 2,377,364 - ABIE40_RS12190 0.68 -0.1 2,377,407 + ABIE40_RS12190 0.76 -0.2 2,377,407 + ABIE40_RS12190 0.76 +0.2 2,377,407 + ABIE40_RS12190 0.76 +0.0 2,377,407 + ABIE40_RS12190 0.76 -2.5 2,377,408 - ABIE40_RS12190 0.77 +0.2 2,377,408 - ABIE40_RS12190 0.77 +0.2 2,377,408 - ABIE40_RS12190 0.77 +0.8 2,377,498 + -0.3 2,377,498 + -1.1 2,377,498 + -0.2 2,377,498 + -0.1 2,377,499 - +0.2 2,377,499 - +1.1 2,377,499 - -1.4 2,377,530 + -1.4 2,377,530 + -0.2 2,377,531 - -0.6 2,377,562 + +0.7 2,377,562 + -0.2 2,377,562 + +0.3 2,377,562 + -0.8 2,377,563 - +0.2 2,377,563 - +0.3 2,377,563 - -3.9 2,377,565 + -0.5 2,377,565 + -0.0 2,377,566 - -0.1 2,377,581 - +0.4 2,377,581 - -0.3 2,377,581 - -0.5
Or see this region's nucleotide sequence