Experiment: L-Lysine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE40_RS08635 and ABIE40_RS08640 are separated by 107 nucleotides ABIE40_RS08640 and ABIE40_RS08645 are separated by 223 nucleotides ABIE40_RS08645 and ABIE40_RS08650 are separated by 64 nucleotides
ABIE40_RS08635: ABIE40_RS08635 - class I poly(R)-hydroxyalkanoic acid synthase, at 1,666,201 to 1,668,078
_RS08635
ABIE40_RS08640: ABIE40_RS08640 - hypothetical protein, at 1,668,186 to 1,668,602
_RS08640
ABIE40_RS08645: ABIE40_RS08645 - LL-diaminopimelate aminotransferase, at 1,668,826 to 1,670,043
_RS08645
ABIE40_RS08650: ABIE40_RS08650 - homoserine dehydrogenase, at 1,670,108 to 1,671,430
_RS08650
Position (kb)
1668
1669
1670
1671 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1667.964 kb on + strand at 1667.964 kb on + strand at 1667.964 kb on + strand at 1667.964 kb on + strand at 1667.964 kb on + strand at 1667.965 kb on - strand at 1667.965 kb on - strand at 1667.966 kb on + strand at 1667.966 kb on + strand at 1667.966 kb on + strand at 1667.966 kb on + strand at 1667.966 kb on + strand at 1667.966 kb on + strand at 1667.967 kb on - strand at 1667.967 kb on - strand at 1667.967 kb on - strand at 1667.967 kb on - strand at 1667.985 kb on + strand at 1668.074 kb on + strand at 1668.074 kb on + strand at 1668.074 kb on + strand at 1668.075 kb on - strand at 1668.075 kb on - strand at 1668.123 kb on + strand at 1668.123 kb on + strand at 1668.124 kb on - strand at 1668.148 kb on + strand at 1668.236 kb on + strand, within ABIE40_RS08640 at 1668.237 kb on - strand, within ABIE40_RS08640 at 1668.546 kb on + strand, within ABIE40_RS08640 at 1668.600 kb on + strand at 1668.600 kb on + strand at 1668.600 kb on + strand at 1668.601 kb on - strand at 1668.729 kb on + strand at 1668.730 kb on - strand at 1668.730 kb on - strand at 1668.755 kb on + strand at 1668.862 kb on + strand at 1668.862 kb on + strand at 1668.862 kb on + strand at 1668.862 kb on + strand at 1668.862 kb on + strand at 1668.863 kb on - strand at 1669.015 kb on + strand, within ABIE40_RS08645 at 1669.015 kb on + strand, within ABIE40_RS08645 at 1669.063 kb on + strand, within ABIE40_RS08645 at 1669.066 kb on + strand, within ABIE40_RS08645 at 1669.066 kb on + strand, within ABIE40_RS08645 at 1669.066 kb on + strand, within ABIE40_RS08645 at 1669.066 kb on + strand, within ABIE40_RS08645 at 1669.067 kb on - strand, within ABIE40_RS08645 at 1669.201 kb on + strand, within ABIE40_RS08645 at 1669.342 kb on + strand, within ABIE40_RS08645 at 1669.384 kb on + strand, within ABIE40_RS08645 at 1669.384 kb on + strand, within ABIE40_RS08645 at 1669.622 kb on - strand, within ABIE40_RS08645 at 1669.918 kb on + strand, within ABIE40_RS08645 at 1669.918 kb on + strand, within ABIE40_RS08645 at 1669.918 kb on + strand, within ABIE40_RS08645 at 1669.918 kb on + strand, within ABIE40_RS08645 at 1669.919 kb on - strand, within ABIE40_RS08645 at 1669.919 kb on - strand, within ABIE40_RS08645 at 1670.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lysine (C) remove 1,667,964 + -1.6 1,667,964 + -2.6 1,667,964 + -3.0 1,667,964 + -2.0 1,667,964 + -2.2 1,667,965 - -3.3 1,667,965 - +1.8 1,667,966 + -3.6 1,667,966 + -2.3 1,667,966 + -2.9 1,667,966 + -2.1 1,667,966 + -2.5 1,667,966 + -3.5 1,667,967 - -1.4 1,667,967 - -1.7 1,667,967 - -2.3 1,667,967 - -1.0 1,667,985 + -1.9 1,668,074 + -0.1 1,668,074 + +1.0 1,668,074 + -1.7 1,668,075 - +0.9 1,668,075 - -0.3 1,668,123 + +0.4 1,668,123 + +0.5 1,668,124 - -1.5 1,668,148 + +1.7 1,668,236 + ABIE40_RS08640 0.12 -3.0 1,668,237 - ABIE40_RS08640 0.12 -0.7 1,668,546 + ABIE40_RS08640 0.86 -0.4 1,668,600 + +0.5 1,668,600 + -1.4 1,668,600 + +0.5 1,668,601 - -3.2 1,668,729 + -0.2 1,668,730 - -2.2 1,668,730 - -0.9 1,668,755 + +0.1 1,668,862 + -2.9 1,668,862 + -2.5 1,668,862 + -2.7 1,668,862 + -2.9 1,668,862 + -1.9 1,668,863 - -2.9 1,669,015 + ABIE40_RS08645 0.16 -4.1 1,669,015 + ABIE40_RS08645 0.16 -1.7 1,669,063 + ABIE40_RS08645 0.19 -2.2 1,669,066 + ABIE40_RS08645 0.20 -2.4 1,669,066 + ABIE40_RS08645 0.20 -1.6 1,669,066 + ABIE40_RS08645 0.20 -3.2 1,669,066 + ABIE40_RS08645 0.20 -1.2 1,669,067 - ABIE40_RS08645 0.20 -1.8 1,669,201 + ABIE40_RS08645 0.31 +0.8 1,669,342 + ABIE40_RS08645 0.42 -3.3 1,669,384 + ABIE40_RS08645 0.46 -1.2 1,669,384 + ABIE40_RS08645 0.46 -1.3 1,669,622 - ABIE40_RS08645 0.65 -2.5 1,669,918 + ABIE40_RS08645 0.90 -1.0 1,669,918 + ABIE40_RS08645 0.90 +0.8 1,669,918 + ABIE40_RS08645 0.90 -0.8 1,669,918 + ABIE40_RS08645 0.90 -1.5 1,669,919 - ABIE40_RS08645 0.90 -2.2 1,669,919 - ABIE40_RS08645 0.90 -0.2 1,670,044 + -0.8
Or see this region's nucleotide sequence