Strain Fitness in Escherichia coli BW25113 around b4012

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrrlE and rrfE are separated by 93 nucleotidesrrfE and yjaA are separated by 74 nucleotidesyjaA and yjaB are separated by 62 nucleotidesyjaB and metA are separated by 156 nucleotides b4009: rrlE - 23S ribosomal RNA (NCBI), at 4,208,066 to 4,210,969 rrlE b4010: rrfE - 5S ribosomal RNA (NCBI), at 4,211,063 to 4,211,182 rrfE b4011: yjaA - hypothetical protein (NCBI), at 4,211,257 to 4,211,640 yjaA b4012: yjaB - predicted acetyltransferase (NCBI), at 4,211,703 to 4,212,146 yjaB b4013: metA - homoserine O-succinyltransferase (NCBI), at 4,212,303 to 4,213,232 metA Position (kb) 4211 4212 4213Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4211.358 kb on - strand, within yjaAat 4211.358 kb on - strand, within yjaAat 4211.402 kb on + strand, within yjaAat 4211.605 kb on - strandat 4211.659 kb on - strandat 4211.767 kb on - strand, within yjaBat 4211.973 kb on + strand, within yjaBat 4211.994 kb on - strand, within yjaBat 4211.994 kb on - strand, within yjaBat 4212.254 kb on - strandat 4212.689 kb on - strand, within metAat 4212.908 kb on - strand, within metAat 4212.908 kb on - strand, within metAat 4212.957 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.008 kb on + strand, within metAat 4213.061 kb on - strand, within metAat 4213.068 kb on + strand, within metA

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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4,211,358 - yjaA b4011 0.26 +0.7
4,211,358 - yjaA b4011 0.26 -0.1
4,211,402 + yjaA b4011 0.38 -0.5
4,211,605 - -1.4
4,211,659 - -0.5
4,211,767 - yjaB b4012 0.14 +0.2
4,211,973 + yjaB b4012 0.61 -1.6
4,211,994 - yjaB b4012 0.66 -0.8
4,211,994 - yjaB b4012 0.66 -0.3
4,212,254 - -1.4
4,212,689 - metA b4013 0.42 -1.7
4,212,908 - metA b4013 0.65 -3.1
4,212,908 - metA b4013 0.65 -2.3
4,212,957 + metA b4013 0.70 -1.9
4,213,008 + metA b4013 0.76 -3.5
4,213,008 + metA b4013 0.76 -4.7
4,213,008 + metA b4013 0.76 -1.4
4,213,061 - metA b4013 0.82 -1.5
4,213,068 + metA b4013 0.82 -2.9

Or see this region's nucleotide sequence