Strain Fitness in Escherichia coli BW25113 around b3990

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthtrC and sroH are separated by 1 nucleotidessroH and thiH are separated by 247 nucleotidesthiH and thiG overlap by 4 nucleotidesthiG and thiS are separated by 1 nucleotidesthiS and thiF overlap by 17 nucleotides b3989: htrC - heat shock protein (NCBI), at 4,187,809 to 4,188,348 htrC b4691: sroH - Novel sRNA, function unknown (RefSeq), at 4,188,350 to 4,188,510 sroH b3990: thiH - thiamine biosynthesis protein ThiH (NCBI), at 4,188,758 to 4,189,891 thiH b3991: thiG - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,189,888 to 4,190,658 thiG b4407: thiS - sulfur carrier protein ThiS (NCBI), at 4,190,660 to 4,190,860 thiS b3992: thiF - thiamin biosynthesis, thiazole moiety (VIMSS), at 4,190,844 to 4,191,599 thiF Position (kb) 4188 4189 4190Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4187.774 kb on + strandat 4187.774 kb on + strandat 4187.793 kb on + strandat 4187.896 kb on + strand, within htrCat 4187.904 kb on - strand, within htrCat 4187.904 kb on - strand, within htrCat 4187.916 kb on - strand, within htrCat 4187.949 kb on + strand, within htrCat 4187.957 kb on - strand, within htrCat 4187.957 kb on - strand, within htrCat 4187.994 kb on - strand, within htrCat 4187.994 kb on - strand, within htrCat 4188.013 kb on + strand, within htrCat 4188.013 kb on + strand, within htrCat 4188.034 kb on - strand, within htrCat 4188.034 kb on - strand, within htrCat 4188.049 kb on + strand, within htrCat 4188.066 kb on - strand, within htrCat 4188.087 kb on - strand, within htrCat 4188.087 kb on - strand, within htrCat 4188.087 kb on - strand, within htrCat 4188.130 kb on + strand, within htrCat 4188.150 kb on + strand, within htrCat 4188.160 kb on + strand, within htrCat 4188.168 kb on - strand, within htrCat 4188.168 kb on - strand, within htrCat 4188.196 kb on - strand, within htrCat 4188.239 kb on + strand, within htrCat 4188.309 kb on - strandat 4188.309 kb on - strandat 4188.365 kb on - strandat 4188.365 kb on - strandat 4188.565 kb on + strandat 4188.579 kb on - strandat 4188.751 kb on - strandat 4188.765 kb on - strandat 4188.812 kb on + strandat 4188.870 kb on + strandat 4188.899 kb on - strand, within thiHat 4189.035 kb on - strand, within thiHat 4189.048 kb on + strand, within thiHat 4189.056 kb on - strand, within thiHat 4189.073 kb on - strand, within thiHat 4189.133 kb on - strand, within thiHat 4189.334 kb on - strand, within thiHat 4189.351 kb on - strand, within thiHat 4189.351 kb on - strand, within thiHat 4189.362 kb on - strand, within thiHat 4189.372 kb on - strand, within thiHat 4189.372 kb on - strand, within thiHat 4189.429 kb on + strand, within thiHat 4189.429 kb on - strand, within thiHat 4189.429 kb on - strand, within thiHat 4189.495 kb on + strand, within thiHat 4189.506 kb on + strand, within thiHat 4189.508 kb on + strand, within thiHat 4189.543 kb on - strand, within thiHat 4189.543 kb on - strand, within thiHat 4189.724 kb on + strand, within thiHat 4189.737 kb on - strand, within thiHat 4189.737 kb on - strand, within thiHat 4189.759 kb on - strand, within thiHat 4189.759 kb on - strand, within thiHat 4190.005 kb on - strand, within thiGat 4190.005 kb on - strand, within thiGat 4190.187 kb on - strand, within thiGat 4190.187 kb on - strand, within thiGat 4190.253 kb on + strand, within thiGat 4190.267 kb on - strand, within thiGat 4190.267 kb on - strand, within thiGat 4190.270 kb on + strand, within thiGat 4190.270 kb on + strand, within thiGat 4190.278 kb on - strand, within thiGat 4190.278 kb on - strand, within thiGat 4190.287 kb on - strand, within thiGat 4190.306 kb on - strand, within thiGat 4190.306 kb on - strand, within thiGat 4190.404 kb on + strand, within thiGat 4190.412 kb on - strand, within thiGat 4190.528 kb on - strand, within thiGat 4190.579 kb on + strand, within thiGat 4190.581 kb on + strand, within thiGat 4190.581 kb on + strand, within thiGat 4190.584 kb on - strandat 4190.586 kb on - strandat 4190.791 kb on + strand, within thiSat 4190.820 kb on + strand, within thiSat 4190.830 kb on + strand, within thiSat 4190.860 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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4,187,774 + -0.0
4,187,774 + +0.5
4,187,793 + -1.3
4,187,896 + htrC b3989 0.16 -1.1
4,187,904 - htrC b3989 0.18 +0.2
4,187,904 - htrC b3989 0.18 +0.1
4,187,916 - htrC b3989 0.20 -0.2
4,187,949 + htrC b3989 0.26 +0.5
4,187,957 - htrC b3989 0.27 +0.6
4,187,957 - htrC b3989 0.27 +0.9
4,187,994 - htrC b3989 0.34 +0.4
4,187,994 - htrC b3989 0.34 +0.7
4,188,013 + htrC b3989 0.38 -2.0
4,188,013 + htrC b3989 0.38 +1.6
4,188,034 - htrC b3989 0.42 +0.7
4,188,034 - htrC b3989 0.42 +0.3
4,188,049 + htrC b3989 0.44 -0.1
4,188,066 - htrC b3989 0.48 -1.6
4,188,087 - htrC b3989 0.51 -0.7
4,188,087 - htrC b3989 0.51 +2.4
4,188,087 - htrC b3989 0.51 -0.4
4,188,130 + htrC b3989 0.59 -0.0
4,188,150 + htrC b3989 0.63 +0.6
4,188,160 + htrC b3989 0.65 +0.5
4,188,168 - htrC b3989 0.66 +0.8
4,188,168 - htrC b3989 0.66 -0.2
4,188,196 - htrC b3989 0.72 -1.1
4,188,239 + htrC b3989 0.80 +1.1
4,188,309 - +0.6
4,188,309 - -0.2
4,188,365 - -0.5
4,188,365 - +0.5
4,188,565 + -0.0
4,188,579 - -0.8
4,188,751 - -1.6
4,188,765 - -0.5
4,188,812 + -1.4
4,188,870 + -1.3
4,188,899 - thiH b3990 0.12 -1.6
4,189,035 - thiH b3990 0.24 -1.7
4,189,048 + thiH b3990 0.26 -1.8
4,189,056 - thiH b3990 0.26 -1.4
4,189,073 - thiH b3990 0.28 -1.7
4,189,133 - thiH b3990 0.33 -0.6
4,189,334 - thiH b3990 0.51 -0.2
4,189,351 - thiH b3990 0.52 -1.8
4,189,351 - thiH b3990 0.52 -1.3
4,189,362 - thiH b3990 0.53 -2.3
4,189,372 - thiH b3990 0.54 -0.6
4,189,372 - thiH b3990 0.54 -3.5
4,189,429 + thiH b3990 0.59 -1.6
4,189,429 - thiH b3990 0.59 -0.2
4,189,429 - thiH b3990 0.59 -2.3
4,189,495 + thiH b3990 0.65 -1.6
4,189,506 + thiH b3990 0.66 -1.1
4,189,508 + thiH b3990 0.66 -2.6
4,189,543 - thiH b3990 0.69 -1.8
4,189,543 - thiH b3990 0.69 -3.4
4,189,724 + thiH b3990 0.85 -2.3
4,189,737 - thiH b3990 0.86 +0.0
4,189,737 - thiH b3990 0.86 +1.2
4,189,759 - thiH b3990 0.88 -1.0
4,189,759 - thiH b3990 0.88 -1.4
4,190,005 - thiG b3991 0.15 -1.8
4,190,005 - thiG b3991 0.15 -1.5
4,190,187 - thiG b3991 0.39 +0.7
4,190,187 - thiG b3991 0.39 -1.3
4,190,253 + thiG b3991 0.47 -2.2
4,190,267 - thiG b3991 0.49 -0.5
4,190,267 - thiG b3991 0.49 -0.0
4,190,270 + thiG b3991 0.50 -0.8
4,190,270 + thiG b3991 0.50 -3.6
4,190,278 - thiG b3991 0.51 -1.8
4,190,278 - thiG b3991 0.51 -2.6
4,190,287 - thiG b3991 0.52 -2.2
4,190,306 - thiG b3991 0.54 -2.2
4,190,306 - thiG b3991 0.54 -1.6
4,190,404 + thiG b3991 0.67 -0.5
4,190,412 - thiG b3991 0.68 -2.3
4,190,528 - thiG b3991 0.83 -1.5
4,190,579 + thiG b3991 0.90 -2.2
4,190,581 + thiG b3991 0.90 -2.6
4,190,581 + thiG b3991 0.90 -0.5
4,190,584 - -1.3
4,190,586 - -1.5
4,190,791 + thiS b4407 0.65 -0.8
4,190,820 + thiS b4407 0.80 -0.5
4,190,830 + thiS b4407 0.85 -1.1
4,190,860 - -1.5

Or see this region's nucleotide sequence