Strain Fitness in Escherichia coli BW25113 around b3034

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpdA and yqiB are separated by 24 nucleotidesyqiB and nudF are separated by 0 nucleotidesnudF and tolC are separated by 204 nucleotides b3032: cpdA - cyclic 3',5'-adenosine monophosphate phosphodiesterase (NCBI), at 3,174,028 to 3,174,855 cpdA b3033: yqiB - predicted dehydrogenase (NCBI), at 3,174,880 to 3,175,302 yqiB b3034: nudF - ADP-ribose pyrophosphatase (NCBI), at 3,175,303 to 3,175,932 nudF b3035: tolC - outer membrane channel; specific tolerance to colicin E1; segregation of daughter chromosomes (VIMSS), at 3,176,137 to 3,177,618 tolC Position (kb) 3175 3176Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3174.303 kb on - strand, within cpdAat 3174.332 kb on + strand, within cpdAat 3174.332 kb on + strand, within cpdAat 3174.373 kb on - strand, within cpdAat 3174.373 kb on - strand, within cpdAat 3174.405 kb on + strand, within cpdAat 3174.433 kb on - strand, within cpdAat 3174.457 kb on + strand, within cpdAat 3174.503 kb on + strand, within cpdAat 3174.506 kb on + strand, within cpdAat 3174.647 kb on + strand, within cpdAat 3174.694 kb on + strand, within cpdAat 3174.749 kb on + strand, within cpdAat 3174.749 kb on + strand, within cpdAat 3174.749 kb on + strand, within cpdAat 3174.791 kb on - strandat 3174.825 kb on - strandat 3174.825 kb on - strandat 3174.905 kb on - strandat 3174.915 kb on - strandat 3174.927 kb on - strand, within yqiBat 3175.065 kb on + strand, within yqiBat 3175.116 kb on + strand, within yqiBat 3175.126 kb on + strand, within yqiBat 3175.171 kb on - strand, within yqiBat 3175.226 kb on + strand, within yqiBat 3175.399 kb on - strand, within nudFat 3175.469 kb on - strand, within nudFat 3175.494 kb on - strand, within nudFat 3175.494 kb on - strand, within nudFat 3175.528 kb on - strand, within nudFat 3175.577 kb on + strand, within nudFat 3175.585 kb on + strand, within nudFat 3175.590 kb on - strand, within nudFat 3175.662 kb on + strand, within nudFat 3175.679 kb on + strand, within nudFat 3175.679 kb on + strand, within nudFat 3175.752 kb on - strand, within nudFat 3175.798 kb on + strand, within nudFat 3175.818 kb on - strand, within nudFat 3175.823 kb on - strand, within nudFat 3175.871 kb on - strandat 3176.030 kb on - strandat 3176.031 kb on + strandat 3176.119 kb on - strandat 3176.119 kb on - strandat 3176.173 kb on - strandat 3176.173 kb on - strandat 3176.174 kb on + strandat 3176.182 kb on - strandat 3176.208 kb on - strandat 3176.271 kb on + strandat 3176.271 kb on + strandat 3176.304 kb on + strand, within tolCat 3176.309 kb on + strand, within tolCat 3176.309 kb on + strand, within tolCat 3176.349 kb on - strand, within tolCat 3176.378 kb on + strand, within tolCat 3176.493 kb on + strand, within tolCat 3176.526 kb on - strand, within tolCat 3176.571 kb on + strand, within tolCat 3176.573 kb on + strand, within tolCat 3176.581 kb on - strand, within tolCat 3176.635 kb on + strand, within tolCat 3176.638 kb on - strand, within tolCat 3176.654 kb on - strand, within tolCat 3176.680 kb on - strand, within tolCat 3176.680 kb on - strand, within tolCat 3176.682 kb on + strand, within tolCat 3176.692 kb on - strand, within tolCat 3176.716 kb on + strand, within tolCat 3176.716 kb on + strand, within tolCat 3176.844 kb on - strand, within tolCat 3176.844 kb on - strand, within tolCat 3176.869 kb on - strand, within tolCat 3176.869 kb on - strand, within tolCat 3176.887 kb on + strand, within tolCat 3176.887 kb on + strand, within tolCat 3176.887 kb on + strand, within tolCat 3176.932 kb on + strand, within tolC

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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3,174,303 - cpdA b3032 0.33 +1.4
3,174,332 + cpdA b3032 0.37 +2.5
3,174,332 + cpdA b3032 0.37 +3.1
3,174,373 - cpdA b3032 0.42 +2.2
3,174,373 - cpdA b3032 0.42 +0.3
3,174,405 + cpdA b3032 0.46 +2.6
3,174,433 - cpdA b3032 0.49 +2.1
3,174,457 + cpdA b3032 0.52 +2.9
3,174,503 + cpdA b3032 0.57 +2.9
3,174,506 + cpdA b3032 0.58 +2.9
3,174,647 + cpdA b3032 0.75 +3.4
3,174,694 + cpdA b3032 0.80 +2.7
3,174,749 + cpdA b3032 0.87 +2.6
3,174,749 + cpdA b3032 0.87 +2.8
3,174,749 + cpdA b3032 0.87 +2.0
3,174,791 - +2.4
3,174,825 - +1.5
3,174,825 - +2.8
3,174,905 - -2.0
3,174,915 - -1.5
3,174,927 - yqiB b3033 0.11 -1.8
3,175,065 + yqiB b3033 0.44 +2.4
3,175,116 + yqiB b3033 0.56 +0.4
3,175,126 + yqiB b3033 0.58 +0.5
3,175,171 - yqiB b3033 0.69 -2.3
3,175,226 + yqiB b3033 0.82 -0.3
3,175,399 - nudF b3034 0.15 -2.0
3,175,469 - nudF b3034 0.26 -2.0
3,175,494 - nudF b3034 0.30 -1.5
3,175,494 - nudF b3034 0.30 -1.6
3,175,528 - nudF b3034 0.36 -1.9
3,175,577 + nudF b3034 0.43 -0.5
3,175,585 + nudF b3034 0.45 -0.5
3,175,590 - nudF b3034 0.46 -0.3
3,175,662 + nudF b3034 0.57 +2.8
3,175,679 + nudF b3034 0.60 +0.0
3,175,679 + nudF b3034 0.60 +0.5
3,175,752 - nudF b3034 0.71 -1.0
3,175,798 + nudF b3034 0.79 +0.1
3,175,818 - nudF b3034 0.82 -0.3
3,175,823 - nudF b3034 0.83 -0.9
3,175,871 - -2.3
3,176,030 - -0.9
3,176,031 + -0.4
3,176,119 - -0.3
3,176,119 - +1.8
3,176,173 - -2.3
3,176,173 - -2.8
3,176,174 + -2.1
3,176,182 - -1.0
3,176,208 - -2.9
3,176,271 + -3.4
3,176,271 + -1.9
3,176,304 + tolC b3035 0.11 +0.1
3,176,309 + tolC b3035 0.12 -1.6
3,176,309 + tolC b3035 0.12 -0.1
3,176,349 - tolC b3035 0.14 -1.6
3,176,378 + tolC b3035 0.16 -2.0
3,176,493 + tolC b3035 0.24 -2.2
3,176,526 - tolC b3035 0.26 -2.3
3,176,571 + tolC b3035 0.29 -0.4
3,176,573 + tolC b3035 0.29 -1.4
3,176,581 - tolC b3035 0.30 -1.5
3,176,635 + tolC b3035 0.34 -3.2
3,176,638 - tolC b3035 0.34 -1.9
3,176,654 - tolC b3035 0.35 -2.8
3,176,680 - tolC b3035 0.37 +0.2
3,176,680 - tolC b3035 0.37 -0.5
3,176,682 + tolC b3035 0.37 -0.5
3,176,692 - tolC b3035 0.37 +0.1
3,176,716 + tolC b3035 0.39 -1.8
3,176,716 + tolC b3035 0.39 -1.0
3,176,844 - tolC b3035 0.48 -0.2
3,176,844 - tolC b3035 0.48 -0.5
3,176,869 - tolC b3035 0.49 -1.2
3,176,869 - tolC b3035 0.49 -2.1
3,176,887 + tolC b3035 0.51 -0.7
3,176,887 + tolC b3035 0.51 -1.4
3,176,887 + tolC b3035 0.51 -2.7
3,176,932 + tolC b3035 0.54 -2.6

Or see this region's nucleotide sequence