Strain Fitness in Escherichia coli BW25113 around b2466

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttktB and ypfG are separated by 94 nucleotidesypfG and yffH are separated by 125 nucleotidesyffH and aegA are separated by 67 nucleotides b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661 tktB b2466: ypfG - hypothetical protein (NCBI), at 2,579,756 to 2,580,799 ypfG b2467: yffH - predicted NUDIX hydrolase (NCBI), at 2,580,925 to 2,581,500 yffH b2468: aegA - fused predicted oxidoreductase: FeS binding subunit/NAD/FAD-binding subunit (NCBI), at 2,581,568 to 2,583,547 aegA Position (kb) 2579 2580 2581Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2578.865 kb on + strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.050 kb on - strand, within tktBat 2579.079 kb on - strand, within tktBat 2579.181 kb on + strand, within tktBat 2579.205 kb on - strand, within tktBat 2579.369 kb on + strand, within tktBat 2579.406 kb on - strand, within tktBat 2579.406 kb on - strand, within tktBat 2579.438 kb on - strand, within tktBat 2579.438 kb on - strand, within tktBat 2579.586 kb on + strandat 2579.702 kb on - strandat 2579.799 kb on - strandat 2579.799 kb on - strandat 2579.825 kb on + strandat 2579.825 kb on + strandat 2580.163 kb on - strand, within ypfGat 2580.219 kb on + strand, within ypfGat 2580.222 kb on - strand, within ypfGat 2580.222 kb on - strand, within ypfGat 2580.223 kb on - strand, within ypfGat 2580.258 kb on - strand, within ypfGat 2580.264 kb on - strand, within ypfGat 2580.517 kb on + strand, within ypfGat 2580.572 kb on + strand, within ypfGat 2580.609 kb on + strand, within ypfGat 2580.609 kb on + strand, within ypfGat 2580.959 kb on + strandat 2580.959 kb on + strandat 2581.059 kb on - strand, within yffHat 2581.099 kb on - strand, within yffHat 2581.124 kb on + strand, within yffHat 2581.325 kb on + strand, within yffHat 2581.335 kb on - strand, within yffHat 2581.342 kb on + strand, within yffHat 2581.350 kb on - strand, within yffHat 2581.369 kb on - strandat 2581.372 kb on + strand, within yffHat 2581.380 kb on - strand, within yffHat 2581.380 kb on - strand, within yffHat 2581.445 kb on + strandat 2581.453 kb on - strandat 2581.453 kb on - strandat 2581.570 kb on + strandat 2581.570 kb on + strandat 2581.662 kb on - strandat 2581.712 kb on - strandat 2581.718 kb on - strandat 2581.718 kb on - strandat 2581.759 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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2,578,865 + tktB b2465 0.60 +0.9
2,579,050 - tktB b2465 0.69 +1.4
2,579,050 - tktB b2465 0.69 +0.0
2,579,050 - tktB b2465 0.69 -1.3
2,579,050 - tktB b2465 0.69 -3.5
2,579,079 - tktB b2465 0.71 +0.1
2,579,181 + tktB b2465 0.76 -0.8
2,579,205 - tktB b2465 0.77 +0.3
2,579,369 + tktB b2465 0.85 +0.1
2,579,406 - tktB b2465 0.87 -0.1
2,579,406 - tktB b2465 0.87 -0.1
2,579,438 - tktB b2465 0.89 -0.4
2,579,438 - tktB b2465 0.89 +0.3
2,579,586 + -0.0
2,579,702 - -0.8
2,579,799 - -0.7
2,579,799 - +1.7
2,579,825 + -0.1
2,579,825 + +0.0
2,580,163 - ypfG b2466 0.39 +0.0
2,580,219 + ypfG b2466 0.44 -1.3
2,580,222 - ypfG b2466 0.45 +1.2
2,580,222 - ypfG b2466 0.45 +1.4
2,580,223 - ypfG b2466 0.45 +0.9
2,580,258 - ypfG b2466 0.48 -0.6
2,580,264 - ypfG b2466 0.49 +0.5
2,580,517 + ypfG b2466 0.73 +1.2
2,580,572 + ypfG b2466 0.78 +0.4
2,580,609 + ypfG b2466 0.82 +0.2
2,580,609 + ypfG b2466 0.82 +1.1
2,580,959 + -0.2
2,580,959 + -0.6
2,581,059 - yffH b2467 0.23 +1.3
2,581,099 - yffH b2467 0.30 +0.6
2,581,124 + yffH b2467 0.35 +0.9
2,581,325 + yffH b2467 0.69 +0.7
2,581,335 - yffH b2467 0.71 -0.3
2,581,342 + yffH b2467 0.72 +0.7
2,581,350 - yffH b2467 0.74 -0.2
2,581,369 - -0.1
2,581,372 + yffH b2467 0.78 -0.5
2,581,380 - yffH b2467 0.79 +1.2
2,581,380 - yffH b2467 0.79 +1.9
2,581,445 + +0.2
2,581,453 - -0.8
2,581,453 - +0.0
2,581,570 + +0.4
2,581,570 + +1.0
2,581,662 - +0.5
2,581,712 - +0.5
2,581,718 - -0.7
2,581,718 - +0.1
2,581,759 + +0.5

Or see this region's nucleotide sequence