Strain Fitness in Escherichia coli BW25113 around b2241

Experiment: L-Malic (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntglpT and glpA are separated by 272 nucleotidesglpA and glpB overlap by 11 nucleotides b2240: glpT - sn-glycerol-3-phosphate transporter (NCBI), at 2,349,038 to 2,350,396 glpT b2241: glpA - sn-glycerol-3-phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)-binding (NCBI), at 2,350,669 to 2,352,297 glpA b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546 glpB Position (kb) 2350 2351 2352 2353Strain fitness (log2 ratio) -1 0 1 2 3at 2349.755 kb on + strand, within glpTat 2349.755 kb on + strand, within glpTat 2349.756 kb on - strand, within glpTat 2349.763 kb on - strand, within glpTat 2349.763 kb on - strand, within glpTat 2349.765 kb on + strand, within glpTat 2349.773 kb on - strand, within glpTat 2349.819 kb on + strand, within glpTat 2349.819 kb on + strand, within glpTat 2349.827 kb on - strand, within glpTat 2349.911 kb on + strand, within glpTat 2349.911 kb on + strand, within glpTat 2349.956 kb on + strand, within glpTat 2349.993 kb on + strand, within glpTat 2350.038 kb on + strand, within glpTat 2350.348 kb on - strandat 2350.348 kb on - strandat 2350.401 kb on - strandat 2350.401 kb on - strandat 2350.783 kb on + strandat 2350.805 kb on + strandat 2350.805 kb on + strandat 2350.830 kb on + strandat 2350.912 kb on + strand, within glpAat 2350.912 kb on + strand, within glpAat 2350.942 kb on - strand, within glpAat 2351.130 kb on - strand, within glpAat 2351.132 kb on + strand, within glpAat 2351.155 kb on + strand, within glpAat 2351.180 kb on - strand, within glpAat 2351.217 kb on + strand, within glpAat 2351.220 kb on + strand, within glpAat 2351.235 kb on - strand, within glpAat 2351.278 kb on + strand, within glpAat 2351.286 kb on - strand, within glpAat 2351.286 kb on - strand, within glpAat 2351.298 kb on + strand, within glpAat 2351.353 kb on + strand, within glpAat 2351.353 kb on + strand, within glpAat 2351.392 kb on - strand, within glpAat 2351.608 kb on - strand, within glpAat 2351.686 kb on - strand, within glpAat 2351.686 kb on - strand, within glpAat 2351.773 kb on + strand, within glpAat 2351.773 kb on + strand, within glpAat 2351.792 kb on + strand, within glpAat 2351.792 kb on + strand, within glpAat 2351.801 kb on + strand, within glpAat 2351.801 kb on + strand, within glpAat 2351.802 kb on + strand, within glpAat 2351.802 kb on + strand, within glpAat 2351.809 kb on - strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.816 kb on + strand, within glpAat 2351.826 kb on + strand, within glpAat 2351.947 kb on + strand, within glpAat 2351.947 kb on + strand, within glpAat 2351.955 kb on - strand, within glpAat 2351.955 kb on - strand, within glpAat 2352.006 kb on - strand, within glpAat 2352.059 kb on + strand, within glpAat 2352.059 kb on + strand, within glpAat 2352.067 kb on - strand, within glpAat 2352.074 kb on - strand, within glpAat 2352.104 kb on + strand, within glpAat 2352.104 kb on + strand, within glpAat 2352.122 kb on + strand, within glpAat 2352.122 kb on + strand, within glpAat 2352.265 kb on + strandat 2352.300 kb on - strandat 2352.326 kb on + strandat 2352.347 kb on + strandat 2352.369 kb on + strandat 2352.440 kb on - strand, within glpBat 2352.447 kb on + strand, within glpBat 2352.461 kb on - strand, within glpBat 2352.463 kb on + strand, within glpBat 2352.463 kb on + strand, within glpBat 2352.506 kb on + strand, within glpBat 2352.506 kb on + strand, within glpBat 2352.547 kb on + strand, within glpBat 2352.631 kb on + strand, within glpBat 2352.650 kb on + strand, within glpBat 2352.650 kb on + strand, within glpBat 2352.787 kb on - strand, within glpBat 2353.281 kb on + strand, within glpBat 2353.291 kb on + strand, within glpBat 2353.291 kb on + strand, within glpB

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Malic (C)
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2,349,755 + glpT b2240 0.53 +0.7
2,349,755 + glpT b2240 0.53 -0.6
2,349,756 - glpT b2240 0.53 +0.8
2,349,763 - glpT b2240 0.53 +1.2
2,349,763 - glpT b2240 0.53 +1.4
2,349,765 + glpT b2240 0.53 +1.1
2,349,773 - glpT b2240 0.54 -0.0
2,349,819 + glpT b2240 0.57 +2.2
2,349,819 + glpT b2240 0.57 +0.0
2,349,827 - glpT b2240 0.58 +0.8
2,349,911 + glpT b2240 0.64 +1.3
2,349,911 + glpT b2240 0.64 +1.2
2,349,956 + glpT b2240 0.68 +2.3
2,349,993 + glpT b2240 0.70 +0.2
2,350,038 + glpT b2240 0.74 +1.3
2,350,348 - +1.1
2,350,348 - +0.8
2,350,401 - +2.0
2,350,401 - +0.6
2,350,783 + +1.1
2,350,805 + +0.5
2,350,805 + -0.5
2,350,830 + +3.0
2,350,912 + glpA b2241 0.15 +0.5
2,350,912 + glpA b2241 0.15 +1.4
2,350,942 - glpA b2241 0.17 +0.2
2,351,130 - glpA b2241 0.28 +0.8
2,351,132 + glpA b2241 0.28 +0.3
2,351,155 + glpA b2241 0.30 +0.3
2,351,180 - glpA b2241 0.31 -1.0
2,351,217 + glpA b2241 0.34 +0.6
2,351,220 + glpA b2241 0.34 +0.8
2,351,235 - glpA b2241 0.35 +0.2
2,351,278 + glpA b2241 0.37 +0.9
2,351,286 - glpA b2241 0.38 -0.1
2,351,286 - glpA b2241 0.38 +0.1
2,351,298 + glpA b2241 0.39 -0.7
2,351,353 + glpA b2241 0.42 -0.5
2,351,353 + glpA b2241 0.42 +0.6
2,351,392 - glpA b2241 0.44 +0.1
2,351,608 - glpA b2241 0.58 +0.6
2,351,686 - glpA b2241 0.62 +1.1
2,351,686 - glpA b2241 0.62 +0.4
2,351,773 + glpA b2241 0.68 -0.8
2,351,773 + glpA b2241 0.68 +0.8
2,351,792 + glpA b2241 0.69 +0.5
2,351,792 + glpA b2241 0.69 +1.1
2,351,801 + glpA b2241 0.69 +2.6
2,351,801 + glpA b2241 0.69 -0.0
2,351,802 + glpA b2241 0.70 +1.2
2,351,802 + glpA b2241 0.70 +0.6
2,351,809 - glpA b2241 0.70 +0.7
2,351,816 + glpA b2241 0.70 -0.1
2,351,816 + glpA b2241 0.70 +0.9
2,351,816 + glpA b2241 0.70 +0.4
2,351,816 + glpA b2241 0.70 +0.2
2,351,826 + glpA b2241 0.71 -0.3
2,351,947 + glpA b2241 0.78 +0.4
2,351,947 + glpA b2241 0.78 +1.6
2,351,955 - glpA b2241 0.79 +1.2
2,351,955 - glpA b2241 0.79 +1.3
2,352,006 - glpA b2241 0.82 +1.2
2,352,059 + glpA b2241 0.85 +1.0
2,352,059 + glpA b2241 0.85 -0.4
2,352,067 - glpA b2241 0.86 +0.3
2,352,074 - glpA b2241 0.86 +1.4
2,352,104 + glpA b2241 0.88 +1.2
2,352,104 + glpA b2241 0.88 +0.4
2,352,122 + glpA b2241 0.89 -0.5
2,352,122 + glpA b2241 0.89 +0.1
2,352,265 + -0.4
2,352,300 - +1.2
2,352,326 + +0.2
2,352,347 + +0.5
2,352,369 + -0.9
2,352,440 - glpB b2242 0.12 +0.5
2,352,447 + glpB b2242 0.13 +0.8
2,352,461 - glpB b2242 0.14 +0.4
2,352,463 + glpB b2242 0.14 -0.2
2,352,463 + glpB b2242 0.14 +1.2
2,352,506 + glpB b2242 0.17 +0.4
2,352,506 + glpB b2242 0.17 +0.8
2,352,547 + glpB b2242 0.21 +1.7
2,352,631 + glpB b2242 0.27 +0.5
2,352,650 + glpB b2242 0.29 -0.0
2,352,650 + glpB b2242 0.29 -0.7
2,352,787 - glpB b2242 0.40 +0.3
2,353,281 + glpB b2242 0.79 -0.5
2,353,291 + glpB b2242 0.80 +0.2
2,353,291 + glpB b2242 0.80 +1.2

Or see this region's nucleotide sequence