Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_15855 and IAI46_15860 are separated by 22 nucleotides IAI46_15860 and IAI46_15865 overlap by 4 nucleotides IAI46_15865 and IAI46_15870 are separated by 430 nucleotides IAI46_15870 and IAI46_15875 are separated by 5 nucleotides
IAI46_15855: IAI46_15855 - flagellum-specific ATP synthase FliI, at 3,330,432 to 3,331,793
_15855
IAI46_15860: IAI46_15860 - flagella biosynthesis chaperone FliJ, at 3,331,816 to 3,332,262
_15860
IAI46_15865: IAI46_15865 - flagellar hook-length control protein FliK, at 3,332,259 to 3,333,473
_15865
IAI46_15870: IAI46_15870 - flagellar basal body-associated protein FliL, at 3,333,904 to 3,334,386
_15870
IAI46_15875: IAI46_15875 - flagellar motor switch protein FliM, at 3,334,392 to 3,335,393
_15875
Position (kb)
3332
3333
3334 Strain fitness (log2 ratio)
-1
0
1 at 3331.318 kb on - strand, within IAI46_15855 at 3331.620 kb on + strand, within IAI46_15855 at 3331.656 kb on + strand, within IAI46_15855 at 3331.720 kb on - strand at 3331.868 kb on + strand, within IAI46_15860 at 3331.869 kb on - strand, within IAI46_15860 at 3331.869 kb on - strand, within IAI46_15860 at 3332.020 kb on + strand, within IAI46_15860 at 3332.020 kb on + strand, within IAI46_15860 at 3332.021 kb on - strand, within IAI46_15860 at 3332.021 kb on - strand, within IAI46_15860 at 3332.252 kb on + strand at 3332.252 kb on + strand at 3332.252 kb on + strand at 3332.253 kb on - strand at 3332.253 kb on - strand at 3332.513 kb on - strand, within IAI46_15865 at 3332.556 kb on - strand, within IAI46_15865 at 3333.028 kb on + strand, within IAI46_15865 at 3333.028 kb on + strand, within IAI46_15865 at 3333.513 kb on + strand at 3333.565 kb on - strand at 3333.612 kb on + strand at 3333.612 kb on + strand at 3333.612 kb on + strand at 3333.612 kb on + strand at 3333.613 kb on - strand at 3333.613 kb on - strand at 3333.616 kb on + strand at 3333.616 kb on + strand at 3333.616 kb on + strand at 3333.617 kb on - strand at 3333.617 kb on - strand at 3333.617 kb on - strand at 3333.617 kb on - strand at 3333.620 kb on + strand at 3333.620 kb on + strand at 3333.621 kb on - strand at 3333.636 kb on + strand at 3333.637 kb on - strand at 3333.680 kb on + strand at 3333.702 kb on - strand at 3333.710 kb on + strand at 3333.710 kb on + strand at 3333.711 kb on - strand at 3333.712 kb on + strand at 3333.713 kb on - strand at 3333.713 kb on - strand at 3333.714 kb on + strand at 3333.756 kb on + strand at 3333.756 kb on + strand at 3333.758 kb on + strand at 3333.758 kb on + strand at 3333.759 kb on - strand at 3333.814 kb on + strand at 3333.822 kb on + strand at 3333.878 kb on + strand at 3333.880 kb on + strand at 3333.880 kb on + strand at 3333.899 kb on + strand at 3333.899 kb on + strand at 3333.899 kb on + strand at 3333.900 kb on - strand at 3333.900 kb on - strand at 3333.903 kb on + strand at 3333.903 kb on + strand at 3333.904 kb on - strand at 3333.920 kb on + strand at 3333.953 kb on + strand, within IAI46_15870 at 3333.954 kb on - strand, within IAI46_15870 at 3333.954 kb on - strand, within IAI46_15870 at 3333.969 kb on - strand, within IAI46_15870 at 3334.078 kb on - strand, within IAI46_15870 at 3334.078 kb on - strand, within IAI46_15870 at 3334.141 kb on + strand, within IAI46_15870 at 3334.141 kb on + strand, within IAI46_15870 at 3334.142 kb on - strand, within IAI46_15870 at 3334.142 kb on - strand, within IAI46_15870 at 3334.142 kb on - strand, within IAI46_15870 at 3334.142 kb on - strand, within IAI46_15870 at 3334.142 kb on - strand, within IAI46_15870 at 3334.144 kb on - strand, within IAI46_15870 at 3334.312 kb on - strand, within IAI46_15870 at 3334.376 kb on + strand at 3334.377 kb on - strand at 3334.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 3,331,318 - IAI46_15855 0.65 -0.4 3,331,620 + IAI46_15855 0.87 +0.2 3,331,656 + IAI46_15855 0.90 +0.2 3,331,720 - -0.1 3,331,868 + IAI46_15860 0.12 +0.8 3,331,869 - IAI46_15860 0.12 -0.0 3,331,869 - IAI46_15860 0.12 -0.2 3,332,020 + IAI46_15860 0.46 +0.1 3,332,020 + IAI46_15860 0.46 +0.4 3,332,021 - IAI46_15860 0.46 -0.6 3,332,021 - IAI46_15860 0.46 +1.3 3,332,252 + +0.6 3,332,252 + +0.6 3,332,252 + -0.0 3,332,253 - -0.0 3,332,253 - +0.6 3,332,513 - IAI46_15865 0.21 -0.1 3,332,556 - IAI46_15865 0.24 -0.9 3,333,028 + IAI46_15865 0.63 -0.0 3,333,028 + IAI46_15865 0.63 -0.9 3,333,513 + +0.2 3,333,565 - +0.7 3,333,612 + +0.4 3,333,612 + +0.9 3,333,612 + +1.2 3,333,612 + +0.4 3,333,613 - +0.3 3,333,613 - +0.7 3,333,616 + -0.7 3,333,616 + +0.6 3,333,616 + +0.4 3,333,617 - +0.5 3,333,617 - +0.2 3,333,617 - +1.0 3,333,617 - +1.2 3,333,620 + +0.2 3,333,620 + +0.1 3,333,621 - +0.2 3,333,636 + -0.5 3,333,637 - +0.4 3,333,680 + +0.3 3,333,702 - +0.4 3,333,710 + +0.1 3,333,710 + +0.7 3,333,711 - +0.9 3,333,712 + +0.9 3,333,713 - -0.4 3,333,713 - +0.3 3,333,714 + -0.0 3,333,756 + -1.0 3,333,756 + +0.8 3,333,758 + -1.4 3,333,758 + -0.2 3,333,759 - -0.6 3,333,814 + -0.9 3,333,822 + +0.4 3,333,878 + -1.3 3,333,880 + +0.9 3,333,880 + -0.6 3,333,899 + +0.4 3,333,899 + +1.0 3,333,899 + +0.3 3,333,900 - +0.1 3,333,900 - +0.7 3,333,903 + +1.1 3,333,903 + +0.4 3,333,904 - +0.6 3,333,920 + +1.2 3,333,953 + IAI46_15870 0.10 -0.2 3,333,954 - IAI46_15870 0.10 +0.5 3,333,954 - IAI46_15870 0.10 +0.5 3,333,969 - IAI46_15870 0.13 +0.4 3,334,078 - IAI46_15870 0.36 +0.6 3,334,078 - IAI46_15870 0.36 +0.6 3,334,141 + IAI46_15870 0.49 +0.0 3,334,141 + IAI46_15870 0.49 +0.4 3,334,142 - IAI46_15870 0.49 +0.4 3,334,142 - IAI46_15870 0.49 +0.4 3,334,142 - IAI46_15870 0.49 -0.4 3,334,142 - IAI46_15870 0.49 +0.4 3,334,142 - IAI46_15870 0.49 +1.2 3,334,144 - IAI46_15870 0.50 -1.0 3,334,312 - IAI46_15870 0.84 +0.1 3,334,376 + +0.8 3,334,377 - -0.2 3,334,391 + +0.8
Or see this region's nucleotide sequence