Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_06295 and IAI46_06300 are separated by 14 nucleotides IAI46_06300 and IAI46_06305 are separated by 17 nucleotides IAI46_06305 and IAI46_06310 overlap by 1 nucleotides IAI46_06310 and IAI46_06315 are separated by 130 nucleotides IAI46_06315 and IAI46_06320 overlap by 4 nucleotides
IAI46_06295: IAI46_06295 - cytochrome ubiquinol oxidase subunit I, at 1,349,444 to 1,351,012
_06295
IAI46_06300: IAI46_06300 - cytochrome d ubiquinol oxidase subunit II, at 1,351,027 to 1,352,166
_06300
IAI46_06305: IAI46_06305 - cytochrome bd-I oxidase subunit CydX, at 1,352,184 to 1,352,297
_06305
IAI46_06310: IAI46_06310 - cyd operon protein YbgE, at 1,352,297 to 1,352,593
_06310
IAI46_06315: IAI46_06315 - tol-pal system-associated acyl-CoA thioesterase, at 1,352,724 to 1,353,128
_06315
IAI46_06320: IAI46_06320 - Tol-Pal system protein TolQ, at 1,353,125 to 1,353,808
_06320
Position (kb)
1351
1352
1353 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1350.039 kb on + strand, within IAI46_06295 at 1350.040 kb on - strand, within IAI46_06295 at 1350.047 kb on - strand, within IAI46_06295 at 1350.056 kb on + strand, within IAI46_06295 at 1350.057 kb on - strand, within IAI46_06295 at 1350.060 kb on - strand, within IAI46_06295 at 1350.151 kb on - strand, within IAI46_06295 at 1350.151 kb on - strand, within IAI46_06295 at 1350.251 kb on - strand, within IAI46_06295 at 1350.293 kb on + strand, within IAI46_06295 at 1350.347 kb on - strand, within IAI46_06295 at 1350.417 kb on - strand, within IAI46_06295 at 1350.448 kb on + strand, within IAI46_06295 at 1350.521 kb on + strand, within IAI46_06295 at 1350.602 kb on + strand, within IAI46_06295 at 1350.603 kb on - strand, within IAI46_06295 at 1350.603 kb on - strand, within IAI46_06295 at 1350.887 kb on + strand at 1351.026 kb on + strand at 1351.043 kb on + strand at 1351.044 kb on - strand at 1351.054 kb on - strand at 1351.147 kb on - strand, within IAI46_06300 at 1351.262 kb on - strand, within IAI46_06300 at 1351.262 kb on - strand, within IAI46_06300 at 1351.286 kb on - strand, within IAI46_06300 at 1351.481 kb on - strand, within IAI46_06300 at 1351.495 kb on + strand, within IAI46_06300 at 1351.495 kb on + strand, within IAI46_06300 at 1351.496 kb on - strand, within IAI46_06300 at 1351.585 kb on + strand, within IAI46_06300 at 1351.586 kb on - strand, within IAI46_06300 at 1351.586 kb on - strand, within IAI46_06300 at 1351.599 kb on + strand, within IAI46_06300 at 1351.712 kb on - strand, within IAI46_06300 at 1351.712 kb on - strand, within IAI46_06300 at 1351.719 kb on + strand, within IAI46_06300 at 1351.719 kb on + strand, within IAI46_06300 at 1351.990 kb on - strand, within IAI46_06300 at 1352.102 kb on - strand at 1352.104 kb on + strand at 1352.164 kb on + strand at 1352.165 kb on - strand at 1352.178 kb on + strand at 1352.178 kb on + strand at 1352.292 kb on - strand at 1352.311 kb on - strand at 1352.325 kb on - strand at 1352.579 kb on + strand at 1352.590 kb on - strand at 1352.603 kb on + strand at 1352.604 kb on - strand at 1352.633 kb on + strand at 1352.634 kb on - strand at 1352.656 kb on + strand at 1352.687 kb on - strand at 1352.688 kb on + strand at 1352.688 kb on + strand at 1352.688 kb on + strand at 1352.691 kb on + strand at 1352.691 kb on + strand at 1352.692 kb on - strand at 1352.700 kb on + strand at 1352.701 kb on - strand at 1352.720 kb on + strand at 1352.729 kb on + strand at 1352.732 kb on + strand at 1352.793 kb on + strand, within IAI46_06315 at 1352.805 kb on + strand, within IAI46_06315 at 1352.919 kb on + strand, within IAI46_06315 at 1353.098 kb on + strand at 1353.098 kb on + strand at 1353.104 kb on + strand at 1353.104 kb on + strand at 1353.105 kb on - strand at 1353.119 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 1,350,039 + IAI46_06295 0.38 +0.2 1,350,040 - IAI46_06295 0.38 +1.5 1,350,047 - IAI46_06295 0.38 -1.9 1,350,056 + IAI46_06295 0.39 -1.5 1,350,057 - IAI46_06295 0.39 +0.7 1,350,060 - IAI46_06295 0.39 -0.7 1,350,151 - IAI46_06295 0.45 +0.3 1,350,151 - IAI46_06295 0.45 +0.3 1,350,251 - IAI46_06295 0.51 +0.2 1,350,293 + IAI46_06295 0.54 -0.8 1,350,347 - IAI46_06295 0.58 -0.1 1,350,417 - IAI46_06295 0.62 +1.6 1,350,448 + IAI46_06295 0.64 +0.1 1,350,521 + IAI46_06295 0.69 +0.0 1,350,602 + IAI46_06295 0.74 +0.6 1,350,603 - IAI46_06295 0.74 -1.0 1,350,603 - IAI46_06295 0.74 -0.6 1,350,887 + +0.2 1,351,026 + -1.1 1,351,043 + -1.1 1,351,044 - +0.1 1,351,054 - +0.1 1,351,147 - IAI46_06300 0.11 -1.4 1,351,262 - IAI46_06300 0.21 +0.4 1,351,262 - IAI46_06300 0.21 +0.2 1,351,286 - IAI46_06300 0.23 +0.0 1,351,481 - IAI46_06300 0.40 -0.6 1,351,495 + IAI46_06300 0.41 -1.5 1,351,495 + IAI46_06300 0.41 +0.7 1,351,496 - IAI46_06300 0.41 +0.1 1,351,585 + IAI46_06300 0.49 -0.0 1,351,586 - IAI46_06300 0.49 -0.2 1,351,586 - IAI46_06300 0.49 -1.6 1,351,599 + IAI46_06300 0.50 -0.6 1,351,712 - IAI46_06300 0.60 -0.2 1,351,712 - IAI46_06300 0.60 -0.1 1,351,719 + IAI46_06300 0.61 -0.6 1,351,719 + IAI46_06300 0.61 +0.0 1,351,990 - IAI46_06300 0.84 -1.4 1,352,102 - +0.4 1,352,104 + -0.5 1,352,164 + +0.5 1,352,165 - -3.2 1,352,178 + -0.1 1,352,178 + -0.1 1,352,292 - +0.8 1,352,311 - +0.3 1,352,325 - +0.2 1,352,579 + +0.6 1,352,590 - -0.1 1,352,603 + -1.0 1,352,604 - +0.8 1,352,633 + -0.8 1,352,634 - -0.8 1,352,656 + -0.1 1,352,687 - -3.1 1,352,688 + +2.0 1,352,688 + -0.6 1,352,688 + +0.3 1,352,691 + +0.3 1,352,691 + -0.4 1,352,692 - -0.6 1,352,700 + +0.1 1,352,701 - -0.1 1,352,720 + -0.0 1,352,729 + +0.2 1,352,732 + +1.6 1,352,793 + IAI46_06315 0.17 +0.1 1,352,805 + IAI46_06315 0.20 +0.4 1,352,919 + IAI46_06315 0.48 -0.4 1,353,098 + -0.7 1,353,098 + -0.4 1,353,104 + +0.2 1,353,104 + -0.2 1,353,105 - -1.7 1,353,119 + -0.1
Or see this region's nucleotide sequence