Experiment: Nickel (II) chloride 80 uM, aerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI46_05040 and IAI46_05045 are separated by 35 nucleotides IAI46_05045 and IAI46_05050 are separated by 97 nucleotides IAI46_05050 and IAI46_05055 are separated by 103 nucleotides IAI46_05055 and IAI46_05060 are separated by 61 nucleotides
IAI46_05040: IAI46_05040 - YbgC/FadM family acyl-CoA thioesterase, at 1,087,636 to 1,088,049
_05040
IAI46_05045: IAI46_05045 - 7-cyano-7-deazaguanine synthase QueC, at 1,088,085 to 1,088,783
_05045
IAI46_05050: IAI46_05050 - SgrR family transcriptional regulator, at 1,088,881 to 1,090,629
_05050
IAI46_05055: IAI46_05055 - HMP-PP phosphatase, at 1,090,733 to 1,091,557
_05055
IAI46_05060: IAI46_05060 - PLP-dependent cysteine synthase family protein, at 1,091,619 to 1,092,659
_05060
Position (kb)
1088
1089
1090
1091 Strain fitness (log2 ratio)
-1
0
1
2 at 1088.144 kb on - strand at 1088.216 kb on - strand, within IAI46_05045 at 1088.216 kb on - strand, within IAI46_05045 at 1088.257 kb on + strand, within IAI46_05045 at 1088.257 kb on + strand, within IAI46_05045 at 1088.257 kb on + strand, within IAI46_05045 at 1088.257 kb on + strand, within IAI46_05045 at 1088.258 kb on - strand, within IAI46_05045 at 1088.258 kb on - strand, within IAI46_05045 at 1088.258 kb on - strand, within IAI46_05045 at 1088.258 kb on - strand, within IAI46_05045 at 1088.258 kb on - strand, within IAI46_05045 at 1088.258 kb on - strand, within IAI46_05045 at 1088.389 kb on + strand, within IAI46_05045 at 1088.452 kb on + strand, within IAI46_05045 at 1088.452 kb on + strand, within IAI46_05045 at 1088.453 kb on - strand, within IAI46_05045 at 1088.453 kb on - strand, within IAI46_05045 at 1088.453 kb on - strand, within IAI46_05045 at 1088.481 kb on - strand, within IAI46_05045 at 1088.483 kb on + strand, within IAI46_05045 at 1088.549 kb on + strand, within IAI46_05045 at 1088.550 kb on - strand, within IAI46_05045 at 1088.610 kb on - strand, within IAI46_05045 at 1088.681 kb on - strand, within IAI46_05045 at 1088.710 kb on + strand, within IAI46_05045 at 1088.711 kb on - strand, within IAI46_05045 at 1088.783 kb on + strand at 1088.840 kb on + strand at 1088.903 kb on - strand at 1088.903 kb on - strand at 1088.903 kb on - strand at 1088.989 kb on - strand at 1089.016 kb on - strand at 1089.024 kb on - strand at 1089.030 kb on - strand at 1089.074 kb on + strand, within IAI46_05050 at 1089.075 kb on - strand, within IAI46_05050 at 1089.075 kb on - strand, within IAI46_05050 at 1089.077 kb on + strand, within IAI46_05050 at 1089.077 kb on + strand, within IAI46_05050 at 1089.190 kb on - strand, within IAI46_05050 at 1089.190 kb on - strand, within IAI46_05050 at 1089.190 kb on - strand, within IAI46_05050 at 1089.306 kb on - strand, within IAI46_05050 at 1089.309 kb on - strand, within IAI46_05050 at 1089.337 kb on + strand, within IAI46_05050 at 1089.338 kb on - strand, within IAI46_05050 at 1089.341 kb on - strand, within IAI46_05050 at 1089.344 kb on - strand, within IAI46_05050 at 1089.344 kb on - strand, within IAI46_05050 at 1089.344 kb on - strand, within IAI46_05050 at 1089.421 kb on + strand, within IAI46_05050 at 1089.422 kb on - strand, within IAI46_05050 at 1089.600 kb on - strand, within IAI46_05050 at 1089.671 kb on - strand, within IAI46_05050 at 1089.735 kb on - strand, within IAI46_05050 at 1089.752 kb on + strand, within IAI46_05050 at 1089.752 kb on + strand, within IAI46_05050 at 1089.752 kb on + strand, within IAI46_05050 at 1089.793 kb on + strand, within IAI46_05050 at 1089.811 kb on + strand, within IAI46_05050 at 1089.917 kb on - strand, within IAI46_05050 at 1090.250 kb on + strand, within IAI46_05050 at 1090.489 kb on + strand at 1090.530 kb on - strand at 1090.590 kb on - strand at 1090.681 kb on + strand at 1090.705 kb on + strand at 1090.706 kb on - strand at 1090.732 kb on + strand at 1090.732 kb on + strand at 1090.732 kb on + strand at 1090.732 kb on + strand at 1090.732 kb on + strand at 1090.732 kb on + strand at 1090.733 kb on - strand at 1090.733 kb on - strand at 1090.733 kb on - strand at 1090.733 kb on - strand at 1090.815 kb on + strand at 1090.943 kb on + strand, within IAI46_05055 at 1090.944 kb on - strand, within IAI46_05055 at 1090.944 kb on - strand, within IAI46_05055 at 1090.992 kb on + strand, within IAI46_05055 at 1090.993 kb on - strand, within IAI46_05055 at 1091.023 kb on + strand, within IAI46_05055 at 1091.203 kb on + strand, within IAI46_05055 at 1091.203 kb on + strand, within IAI46_05055 at 1091.203 kb on + strand, within IAI46_05055 at 1091.204 kb on - strand, within IAI46_05055 at 1091.220 kb on + strand, within IAI46_05055 at 1091.264 kb on + strand, within IAI46_05055 at 1091.297 kb on - strand, within IAI46_05055 at 1091.318 kb on - strand, within IAI46_05055 at 1091.463 kb on + strand, within IAI46_05055 at 1091.463 kb on + strand, within IAI46_05055 at 1091.464 kb on - strand, within IAI46_05055 at 1091.532 kb on - strand at 1091.541 kb on - strand at 1091.541 kb on - strand at 1091.554 kb on - strand at 1091.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 80 uM, aerobic remove 1,088,144 - +0.3 1,088,216 - IAI46_05045 0.19 -0.9 1,088,216 - IAI46_05045 0.19 -0.1 1,088,257 + IAI46_05045 0.25 -0.1 1,088,257 + IAI46_05045 0.25 -0.3 1,088,257 + IAI46_05045 0.25 +0.7 1,088,257 + IAI46_05045 0.25 -1.0 1,088,258 - IAI46_05045 0.25 +1.1 1,088,258 - IAI46_05045 0.25 +0.5 1,088,258 - IAI46_05045 0.25 +0.0 1,088,258 - IAI46_05045 0.25 -0.2 1,088,258 - IAI46_05045 0.25 +0.3 1,088,258 - IAI46_05045 0.25 -0.3 1,088,389 + IAI46_05045 0.43 -0.2 1,088,452 + IAI46_05045 0.53 -0.3 1,088,452 + IAI46_05045 0.53 +0.2 1,088,453 - IAI46_05045 0.53 +1.5 1,088,453 - IAI46_05045 0.53 +0.6 1,088,453 - IAI46_05045 0.53 -0.2 1,088,481 - IAI46_05045 0.57 +0.1 1,088,483 + IAI46_05045 0.57 -0.2 1,088,549 + IAI46_05045 0.66 -0.5 1,088,550 - IAI46_05045 0.67 -0.3 1,088,610 - IAI46_05045 0.75 +0.3 1,088,681 - IAI46_05045 0.85 -0.1 1,088,710 + IAI46_05045 0.89 +0.5 1,088,711 - IAI46_05045 0.90 -0.3 1,088,783 + -1.6 1,088,840 + -0.8 1,088,903 - -0.3 1,088,903 - +0.9 1,088,903 - +0.8 1,088,989 - -0.7 1,089,016 - +0.0 1,089,024 - -0.2 1,089,030 - -1.0 1,089,074 + IAI46_05050 0.11 +0.6 1,089,075 - IAI46_05050 0.11 +0.1 1,089,075 - IAI46_05050 0.11 +0.1 1,089,077 + IAI46_05050 0.11 +0.8 1,089,077 + IAI46_05050 0.11 -0.3 1,089,190 - IAI46_05050 0.18 +0.3 1,089,190 - IAI46_05050 0.18 -0.7 1,089,190 - IAI46_05050 0.18 -0.3 1,089,306 - IAI46_05050 0.24 +0.9 1,089,309 - IAI46_05050 0.24 +0.0 1,089,337 + IAI46_05050 0.26 -0.4 1,089,338 - IAI46_05050 0.26 -1.2 1,089,341 - IAI46_05050 0.26 +0.0 1,089,344 - IAI46_05050 0.26 -0.2 1,089,344 - IAI46_05050 0.26 +0.7 1,089,344 - IAI46_05050 0.26 +0.8 1,089,421 + IAI46_05050 0.31 -0.3 1,089,422 - IAI46_05050 0.31 +0.3 1,089,600 - IAI46_05050 0.41 +0.2 1,089,671 - IAI46_05050 0.45 -0.4 1,089,735 - IAI46_05050 0.49 +1.3 1,089,752 + IAI46_05050 0.50 -0.7 1,089,752 + IAI46_05050 0.50 +0.2 1,089,752 + IAI46_05050 0.50 +0.0 1,089,793 + IAI46_05050 0.52 +0.6 1,089,811 + IAI46_05050 0.53 +0.6 1,089,917 - IAI46_05050 0.59 -0.0 1,090,250 + IAI46_05050 0.78 +0.6 1,090,489 + +0.3 1,090,530 - +1.5 1,090,590 - -0.6 1,090,681 + -0.1 1,090,705 + -0.3 1,090,706 - -0.2 1,090,732 + +0.8 1,090,732 + -0.5 1,090,732 + -0.9 1,090,732 + +0.5 1,090,732 + +0.1 1,090,732 + +1.5 1,090,733 - -0.1 1,090,733 - +0.8 1,090,733 - +1.8 1,090,733 - +0.6 1,090,815 + +0.2 1,090,943 + IAI46_05055 0.25 +1.6 1,090,944 - IAI46_05055 0.26 +0.2 1,090,944 - IAI46_05055 0.26 +0.2 1,090,992 + IAI46_05055 0.31 -0.2 1,090,993 - IAI46_05055 0.32 -0.0 1,091,023 + IAI46_05055 0.35 +0.6 1,091,203 + IAI46_05055 0.57 -1.4 1,091,203 + IAI46_05055 0.57 +0.3 1,091,203 + IAI46_05055 0.57 +0.0 1,091,204 - IAI46_05055 0.57 +0.2 1,091,220 + IAI46_05055 0.59 +0.3 1,091,264 + IAI46_05055 0.64 +1.0 1,091,297 - IAI46_05055 0.68 +0.0 1,091,318 - IAI46_05055 0.71 +0.4 1,091,463 + IAI46_05055 0.88 +0.1 1,091,463 + IAI46_05055 0.88 -0.1 1,091,464 - IAI46_05055 0.89 -0.2 1,091,532 - +0.2 1,091,541 - -1.4 1,091,541 - +0.2 1,091,554 - +0.4 1,091,564 - +0.6
Or see this region's nucleotide sequence