Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_4545

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_4544 and EX28DRAFT_4545 are separated by 15 nucleotidesEX28DRAFT_4545 and EX28DRAFT_4546 are separated by 472 nucleotides EX28DRAFT_4544: EX28DRAFT_4544 - phosphoribosylamine--glycine ligase (EC 6.3.4.13), at 25,859 to 27,151 _4544 EX28DRAFT_4545: EX28DRAFT_4545 - AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful), at 27,167 to 28,756 _4545 EX28DRAFT_4546: EX28DRAFT_4546 - hypothetical protein, at 29,229 to 29,335 _4546 Position (kb) 27 28 29Strain fitness (log2 ratio) -2 -1 0 1at 27.169 kb on - strandat 28.041 kb on + strand, within EX28DRAFT_4545at 28.041 kb on + strand, within EX28DRAFT_4545at 28.782 kb on - strandat 28.782 kb on - strandat 28.782 kb on - strandat 28.794 kb on - strandat 28.794 kb on - strandat 28.794 kb on - strandat 28.846 kb on - strandat 28.849 kb on - strandat 28.932 kb on - strandat 28.942 kb on - strandat 29.129 kb on + strandat 29.132 kb on + strandat 29.134 kb on + strandat 29.134 kb on + strandat 29.134 kb on + strandat 29.134 kb on + strandat 29.189 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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27,169 - -0.6
28,041 + EX28DRAFT_4545 0.55 -0.5
28,041 + EX28DRAFT_4545 0.55 -2.3
28,782 - +0.5
28,782 - -0.1
28,782 - -0.6
28,794 - +0.3
28,794 - +0.6
28,794 - +0.5
28,846 - -0.9
28,849 - +0.3
28,932 - -0.3
28,942 - -0.9
29,129 + +0.1
29,132 + -0.2
29,134 + -0.0
29,134 + +0.2
29,134 + +0.7
29,134 + -0.9
29,189 - +1.5

Or see this region's nucleotide sequence