Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4532 and EX28DRAFT_4533 are separated by 1 nucleotides EX28DRAFT_4533 and EX28DRAFT_4534 overlap by 17 nucleotides EX28DRAFT_4534 and EX28DRAFT_4535 overlap by 14 nucleotides EX28DRAFT_4535 and EX28DRAFT_4536 overlap by 1 nucleotides
EX28DRAFT_4532: EX28DRAFT_4532 - Uncharacterized enzyme of thiazole biosynthesis, at 16,385 to 17,155
_4532
EX28DRAFT_4533: EX28DRAFT_4533 - thiamine biosynthesis protein ThiS, at 17,157 to 17,357
_4533
EX28DRAFT_4534: EX28DRAFT_4534 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2, at 17,341 to 18,096
_4534
EX28DRAFT_4535: EX28DRAFT_4535 - thiamine-phosphate diphosphorylase, at 18,083 to 18,724
_4535
EX28DRAFT_4536: EX28DRAFT_4536 - phosphomethylpyrimidine synthase, at 18,724 to 20,619
_4536
Position (kb) 18
19 Strain fitness (log2 ratio) -2
-1
0
1
2 at 17.147 kb on - strand at 17.147 kb on - strand at 17.151 kb on - strand at 17.509 kb on + strand, within EX28DRAFT_4534 at 17.671 kb on - strand, within EX28DRAFT_4534 at 18.244 kb on + strand, within EX28DRAFT_4535 at 18.265 kb on + strand, within EX28DRAFT_4535 at 18.351 kb on + strand, within EX28DRAFT_4535 at 18.351 kb on + strand, within EX28DRAFT_4535 at 18.352 kb on - strand, within EX28DRAFT_4535 at 18.352 kb on - strand, within EX28DRAFT_4535 at 18.352 kb on - strand, within EX28DRAFT_4535 at 18.524 kb on - strand, within EX28DRAFT_4535 at 18.687 kb on - strand at 18.720 kb on + strand at 18.720 kb on + strand at 18.720 kb on + strand at 18.720 kb on + strand at 18.720 kb on + strand at 18.721 kb on - strand at 18.721 kb on - strand at 18.721 kb on - strand at 18.721 kb on - strand at 18.725 kb on + strand at 18.726 kb on - strand at 18.746 kb on + strand at 18.873 kb on + strand at 18.874 kb on - strand at 19.070 kb on + strand, within EX28DRAFT_4536 at 19.242 kb on - strand, within EX28DRAFT_4536 at 19.242 kb on - strand, within EX28DRAFT_4536 at 19.343 kb on + strand, within EX28DRAFT_4536 at 19.343 kb on + strand, within EX28DRAFT_4536 at 19.344 kb on - strand, within EX28DRAFT_4536 at 19.463 kb on + strand, within EX28DRAFT_4536 at 19.464 kb on - strand, within EX28DRAFT_4536
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 17,147 - +0.5 17,147 - -0.6 17,151 - -0.2 17,509 + EX28DRAFT_4534 0.22 -1.1 17,671 - EX28DRAFT_4534 0.44 -0.2 18,244 + EX28DRAFT_4535 0.25 +0.2 18,265 + EX28DRAFT_4535 0.28 +0.9 18,351 + EX28DRAFT_4535 0.42 +1.1 18,351 + EX28DRAFT_4535 0.42 -1.9 18,352 - EX28DRAFT_4535 0.42 -0.1 18,352 - EX28DRAFT_4535 0.42 +0.8 18,352 - EX28DRAFT_4535 0.42 +0.8 18,524 - EX28DRAFT_4535 0.69 -0.1 18,687 - -0.9 18,720 + -0.3 18,720 + +0.8 18,720 + -0.6 18,720 + +0.3 18,720 + -1.7 18,721 - +0.6 18,721 - -0.4 18,721 - -1.3 18,721 - -0.2 18,725 + -1.7 18,726 - +0.6 18,746 + +2.2 18,873 + -2.4 18,874 - +0.3 19,070 + EX28DRAFT_4536 0.18 -0.7 19,242 - EX28DRAFT_4536 0.27 -0.3 19,242 - EX28DRAFT_4536 0.27 -0.8 19,343 + EX28DRAFT_4536 0.33 +0.2 19,343 + EX28DRAFT_4536 0.33 -1.0 19,344 - EX28DRAFT_4536 0.33 +0.5 19,463 + EX28DRAFT_4536 0.39 -0.5 19,464 - EX28DRAFT_4536 0.39 -0.5
Or see this region's nucleotide sequence