Strain Fitness in Enterobacter asburiae PDN3 around EX28DRAFT_4425

Experiment: M9 with casaminos and trichloroethylene 76.3 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntEX28DRAFT_4423 and EX28DRAFT_4424 are separated by 96 nucleotidesEX28DRAFT_4424 and EX28DRAFT_4425 overlap by 18 nucleotidesEX28DRAFT_4425 and EX28DRAFT_4426 are separated by 196 nucleotidesEX28DRAFT_4426 and EX28DRAFT_4427 are separated by 9 nucleotides EX28DRAFT_4423: EX28DRAFT_4423 - RNA, at 91,074 to 91,184 _4423 EX28DRAFT_4424: EX28DRAFT_4424 - Transcriptional regulator, at 91,281 to 92,198 _4424 EX28DRAFT_4425: EX28DRAFT_4425 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes, at 92,181 to 93,581 _4425 EX28DRAFT_4426: EX28DRAFT_4426 - transcriptional regulator, TetR family, at 93,778 to 94,416 _4426 EX28DRAFT_4427: EX28DRAFT_4427 - Protein of unknown function (DUF1422), at 94,426 to 94,785 _4427 Position (kb) 92 93 94Strain fitness (log2 ratio) -2 -1 0 1 2at 91.184 kb on + strandat 91.193 kb on + strandat 92.267 kb on - strandat 92.528 kb on + strand, within EX28DRAFT_4425at 92.530 kb on + strand, within EX28DRAFT_4425at 92.686 kb on + strand, within EX28DRAFT_4425at 92.686 kb on + strand, within EX28DRAFT_4425at 92.686 kb on + strand, within EX28DRAFT_4425at 93.041 kb on + strand, within EX28DRAFT_4425at 93.302 kb on + strand, within EX28DRAFT_4425at 93.368 kb on - strand, within EX28DRAFT_4425at 93.465 kb on - strandat 93.465 kb on - strandat 93.549 kb on - strandat 93.603 kb on + strandat 93.651 kb on - strandat 93.651 kb on - strandat 93.651 kb on - strandat 93.693 kb on - strandat 93.693 kb on - strandat 93.726 kb on - strandat 93.741 kb on + strandat 93.742 kb on - strandat 93.743 kb on + strandat 93.743 kb on + strandat 94.193 kb on - strand, within EX28DRAFT_4426at 94.366 kb on + strandat 94.495 kb on - strand, within EX28DRAFT_4427at 94.567 kb on + strand, within EX28DRAFT_4427

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM
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91,184 + -0.3
91,193 + +1.4
92,267 - -0.5
92,528 + EX28DRAFT_4425 0.25 -0.2
92,530 + EX28DRAFT_4425 0.25 -0.9
92,686 + EX28DRAFT_4425 0.36 -1.8
92,686 + EX28DRAFT_4425 0.36 -0.5
92,686 + EX28DRAFT_4425 0.36 -1.3
93,041 + EX28DRAFT_4425 0.61 -2.0
93,302 + EX28DRAFT_4425 0.80 -0.7
93,368 - EX28DRAFT_4425 0.85 -0.1
93,465 - -2.0
93,465 - -0.9
93,549 - -0.5
93,603 + -0.2
93,651 - -0.4
93,651 - +0.3
93,651 - +0.3
93,693 - +1.9
93,693 - +0.0
93,726 - +0.1
93,741 + -0.2
93,742 - -0.1
93,743 + -0.1
93,743 + -0.1
94,193 - EX28DRAFT_4426 0.65 -0.1
94,366 + -1.1
94,495 - EX28DRAFT_4427 0.19 +1.2
94,567 + EX28DRAFT_4427 0.39 +0.2

Or see this region's nucleotide sequence