Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4423 and EX28DRAFT_4424 are separated by 96 nucleotides EX28DRAFT_4424 and EX28DRAFT_4425 overlap by 18 nucleotides EX28DRAFT_4425 and EX28DRAFT_4426 are separated by 196 nucleotides EX28DRAFT_4426 and EX28DRAFT_4427 are separated by 9 nucleotides
EX28DRAFT_4423: EX28DRAFT_4423 - RNA, at 91,074 to 91,184
_4423
EX28DRAFT_4424: EX28DRAFT_4424 - Transcriptional regulator, at 91,281 to 92,198
_4424
EX28DRAFT_4425: EX28DRAFT_4425 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes, at 92,181 to 93,581
_4425
EX28DRAFT_4426: EX28DRAFT_4426 - transcriptional regulator, TetR family, at 93,778 to 94,416
_4426
EX28DRAFT_4427: EX28DRAFT_4427 - Protein of unknown function (DUF1422), at 94,426 to 94,785
_4427
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 91.184 kb on + strand at 91.193 kb on + strand at 92.267 kb on - strand at 92.528 kb on + strand, within EX28DRAFT_4425 at 92.530 kb on + strand, within EX28DRAFT_4425 at 92.686 kb on + strand, within EX28DRAFT_4425 at 92.686 kb on + strand, within EX28DRAFT_4425 at 92.686 kb on + strand, within EX28DRAFT_4425 at 93.041 kb on + strand, within EX28DRAFT_4425 at 93.302 kb on + strand, within EX28DRAFT_4425 at 93.368 kb on - strand, within EX28DRAFT_4425 at 93.465 kb on - strand at 93.465 kb on - strand at 93.549 kb on - strand at 93.603 kb on + strand at 93.651 kb on - strand at 93.651 kb on - strand at 93.651 kb on - strand at 93.693 kb on - strand at 93.693 kb on - strand at 93.726 kb on - strand at 93.741 kb on + strand at 93.742 kb on - strand at 93.743 kb on + strand at 93.743 kb on + strand at 94.193 kb on - strand, within EX28DRAFT_4426 at 94.366 kb on + strand at 94.495 kb on - strand, within EX28DRAFT_4427 at 94.567 kb on + strand, within EX28DRAFT_4427
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 91,184 + -0.3 91,193 + +1.4 92,267 - -0.5 92,528 + EX28DRAFT_4425 0.25 -0.2 92,530 + EX28DRAFT_4425 0.25 -0.9 92,686 + EX28DRAFT_4425 0.36 -1.8 92,686 + EX28DRAFT_4425 0.36 -0.5 92,686 + EX28DRAFT_4425 0.36 -1.3 93,041 + EX28DRAFT_4425 0.61 -2.0 93,302 + EX28DRAFT_4425 0.80 -0.7 93,368 - EX28DRAFT_4425 0.85 -0.1 93,465 - -2.0 93,465 - -0.9 93,549 - -0.5 93,603 + -0.2 93,651 - -0.4 93,651 - +0.3 93,651 - +0.3 93,693 - +1.9 93,693 - +0.0 93,726 - +0.1 93,741 + -0.2 93,742 - -0.1 93,743 + -0.1 93,743 + -0.1 94,193 - EX28DRAFT_4426 0.65 -0.1 94,366 + -1.1 94,495 - EX28DRAFT_4427 0.19 +1.2 94,567 + EX28DRAFT_4427 0.39 +0.2
Or see this region's nucleotide sequence