Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4264 and EX28DRAFT_4265 are separated by 172 nucleotides EX28DRAFT_4265 and EX28DRAFT_4266 are separated by 115 nucleotides EX28DRAFT_4266 and EX28DRAFT_4267 are separated by 69 nucleotides EX28DRAFT_4267 and EX28DRAFT_4268 are separated by 117 nucleotides
EX28DRAFT_4264: EX28DRAFT_4264 - F plasmid transfer operon, TraF, protein, at 68,948 to 70,258
_4264
EX28DRAFT_4265: EX28DRAFT_4265 - Uncharacterized conserved protein, contains double-stranded beta-helix domain, at 70,431 to 70,919
_4265
EX28DRAFT_4266: EX28DRAFT_4266 - Fe2+/Zn2+ uptake regulation proteins, at 71,035 to 71,547
_4266
EX28DRAFT_4267: EX28DRAFT_4267 - Uncharacterized protein conserved in bacteria, at 71,617 to 71,829
_4267
EX28DRAFT_4268: EX28DRAFT_4268 - putative efflux protein, MATE family, at 71,947 to 73,284
_4268
Position (kb) 71
72 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 70.123 kb on - strand, within EX28DRAFT_4264 at 70.123 kb on - strand, within EX28DRAFT_4264 at 70.181 kb on - strand at 70.272 kb on + strand at 70.272 kb on + strand at 70.273 kb on - strand at 70.273 kb on - strand at 70.273 kb on - strand at 70.323 kb on + strand at 70.336 kb on - strand at 70.339 kb on + strand at 70.340 kb on - strand at 70.340 kb on - strand at 70.371 kb on - strand at 70.371 kb on - strand at 70.414 kb on - strand at 70.513 kb on + strand, within EX28DRAFT_4265 at 70.513 kb on + strand, within EX28DRAFT_4265 at 70.515 kb on + strand, within EX28DRAFT_4265 at 70.516 kb on - strand, within EX28DRAFT_4265 at 70.535 kb on - strand, within EX28DRAFT_4265 at 70.535 kb on - strand, within EX28DRAFT_4265 at 70.535 kb on - strand, within EX28DRAFT_4265 at 70.672 kb on + strand, within EX28DRAFT_4265 at 70.673 kb on - strand, within EX28DRAFT_4265 at 70.712 kb on - strand, within EX28DRAFT_4265 at 70.747 kb on + strand, within EX28DRAFT_4265 at 70.747 kb on + strand, within EX28DRAFT_4265 at 70.787 kb on + strand, within EX28DRAFT_4265 at 70.924 kb on + strand at 70.924 kb on + strand at 70.925 kb on - strand at 70.953 kb on - strand at 70.953 kb on - strand at 70.995 kb on - strand at 71.015 kb on + strand at 71.015 kb on + strand at 71.040 kb on + strand at 71.389 kb on - strand, within EX28DRAFT_4266 at 71.391 kb on + strand, within EX28DRAFT_4266 at 71.392 kb on - strand, within EX28DRAFT_4266 at 71.587 kb on + strand at 71.587 kb on + strand at 71.588 kb on - strand at 71.598 kb on - strand at 71.627 kb on + strand at 71.654 kb on - strand, within EX28DRAFT_4267 at 71.742 kb on + strand, within EX28DRAFT_4267 at 71.821 kb on - strand at 72.132 kb on + strand, within EX28DRAFT_4268 at 72.183 kb on - strand, within EX28DRAFT_4268 at 72.265 kb on - strand, within EX28DRAFT_4268 at 72.265 kb on - strand, within EX28DRAFT_4268 at 72.389 kb on + strand, within EX28DRAFT_4268 at 72.412 kb on + strand, within EX28DRAFT_4268 at 72.420 kb on - strand, within EX28DRAFT_4268
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 70,123 - EX28DRAFT_4264 0.90 +0.4 70,123 - EX28DRAFT_4264 0.90 -0.1 70,181 - +0.3 70,272 + +0.2 70,272 + -0.0 70,273 - +0.1 70,273 - +0.5 70,273 - -1.9 70,323 + +0.6 70,336 - -1.2 70,339 + -0.5 70,340 - -0.1 70,340 - +0.4 70,371 - +1.5 70,371 - -0.6 70,414 - -0.0 70,513 + EX28DRAFT_4265 0.17 +3.2 70,513 + EX28DRAFT_4265 0.17 +0.1 70,515 + EX28DRAFT_4265 0.17 -0.8 70,516 - EX28DRAFT_4265 0.17 -0.2 70,535 - EX28DRAFT_4265 0.21 +1.4 70,535 - EX28DRAFT_4265 0.21 +0.4 70,535 - EX28DRAFT_4265 0.21 +0.2 70,672 + EX28DRAFT_4265 0.49 -0.1 70,673 - EX28DRAFT_4265 0.49 +0.0 70,712 - EX28DRAFT_4265 0.57 -0.3 70,747 + EX28DRAFT_4265 0.65 -0.4 70,747 + EX28DRAFT_4265 0.65 +0.4 70,787 + EX28DRAFT_4265 0.73 +0.7 70,924 + -2.2 70,924 + -0.8 70,925 - -0.4 70,953 - -0.5 70,953 - +0.4 70,995 - -0.2 71,015 + +0.8 71,015 + +0.5 71,040 + -0.3 71,389 - EX28DRAFT_4266 0.69 +0.6 71,391 + EX28DRAFT_4266 0.69 +0.4 71,392 - EX28DRAFT_4266 0.70 -1.0 71,587 + +0.3 71,587 + -0.2 71,588 - -0.2 71,598 - -0.3 71,627 + -0.0 71,654 - EX28DRAFT_4267 0.17 -0.3 71,742 + EX28DRAFT_4267 0.59 -1.4 71,821 - +0.1 72,132 + EX28DRAFT_4268 0.14 -0.8 72,183 - EX28DRAFT_4268 0.18 +0.2 72,265 - EX28DRAFT_4268 0.24 +1.4 72,265 - EX28DRAFT_4268 0.24 +0.5 72,389 + EX28DRAFT_4268 0.33 +0.3 72,412 + EX28DRAFT_4268 0.35 -0.9 72,420 - EX28DRAFT_4268 0.35 +0.4
Or see this region's nucleotide sequence