Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4156 and EX28DRAFT_4157 overlap by 8 nucleotides EX28DRAFT_4157 and EX28DRAFT_4158 overlap by 8 nucleotides EX28DRAFT_4158 and EX28DRAFT_4159 are separated by 144 nucleotides EX28DRAFT_4159 and EX28DRAFT_4160 are separated by 142 nucleotides
EX28DRAFT_4156: EX28DRAFT_4156 - ribulose-phosphate 3-epimerase, at 137,329 to 138,006
_4156
EX28DRAFT_4157: EX28DRAFT_4157 - 2-phosphoglycolate phosphatase, prokaryotic, at 137,999 to 138,760
_4157
EX28DRAFT_4158: EX28DRAFT_4158 - tryptophanyl-tRNA synthetase (EC 6.1.1.2), at 138,753 to 139,757
_4158
EX28DRAFT_4159: EX28DRAFT_4159 - Protein of unknown function (DUF4223), at 139,902 to 140,072
_4159
EX28DRAFT_4160: EX28DRAFT_4160 - uroporphyrin-III C-methyltransferase, at 140,215 to 141,588
_4160
Position (kb) 138
139
140 Strain fitness (log2 ratio) -2
-1
0
1
2 at 137.993 kb on + strand at 137.993 kb on + strand at 138.004 kb on + strand at 138.099 kb on - strand, within EX28DRAFT_4157 at 138.099 kb on - strand, within EX28DRAFT_4157 at 138.138 kb on + strand, within EX28DRAFT_4157 at 138.138 kb on + strand, within EX28DRAFT_4157 at 138.139 kb on - strand, within EX28DRAFT_4157 at 138.259 kb on + strand, within EX28DRAFT_4157 at 138.305 kb on + strand, within EX28DRAFT_4157 at 138.458 kb on + strand, within EX28DRAFT_4157 at 138.458 kb on + strand, within EX28DRAFT_4157 at 138.543 kb on - strand, within EX28DRAFT_4157 at 138.589 kb on + strand, within EX28DRAFT_4157 at 138.590 kb on - strand, within EX28DRAFT_4157 at 138.636 kb on - strand, within EX28DRAFT_4157 at 138.636 kb on - strand, within EX28DRAFT_4157 at 138.647 kb on + strand, within EX28DRAFT_4157 at 138.647 kb on + strand, within EX28DRAFT_4157 at 138.666 kb on + strand, within EX28DRAFT_4157 at 138.774 kb on - strand at 138.813 kb on + strand at 138.814 kb on - strand at 139.051 kb on - strand, within EX28DRAFT_4158 at 139.051 kb on - strand, within EX28DRAFT_4158 at 139.101 kb on + strand, within EX28DRAFT_4158 at 139.777 kb on + strand at 139.777 kb on + strand at 139.778 kb on - strand at 139.778 kb on - strand at 139.778 kb on - strand at 139.903 kb on + strand at 139.969 kb on + strand, within EX28DRAFT_4159 at 139.970 kb on - strand, within EX28DRAFT_4159 at 139.975 kb on + strand, within EX28DRAFT_4159 at 139.976 kb on - strand, within EX28DRAFT_4159 at 139.976 kb on - strand, within EX28DRAFT_4159 at 139.976 kb on - strand, within EX28DRAFT_4159 at 139.986 kb on - strand, within EX28DRAFT_4159 at 140.002 kb on - strand, within EX28DRAFT_4159 at 140.088 kb on + strand at 140.106 kb on + strand at 140.148 kb on - strand at 140.209 kb on + strand at 140.210 kb on - strand at 140.210 kb on - strand at 140.303 kb on - strand at 140.303 kb on - strand at 140.447 kb on + strand, within EX28DRAFT_4160 at 140.448 kb on - strand, within EX28DRAFT_4160 at 140.448 kb on - strand, within EX28DRAFT_4160 at 140.599 kb on - strand, within EX28DRAFT_4160
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 137,993 + +0.6 137,993 + -0.2 138,004 + +0.7 138,099 - EX28DRAFT_4157 0.13 -0.3 138,099 - EX28DRAFT_4157 0.13 -1.3 138,138 + EX28DRAFT_4157 0.18 -0.0 138,138 + EX28DRAFT_4157 0.18 -0.3 138,139 - EX28DRAFT_4157 0.18 -0.5 138,259 + EX28DRAFT_4157 0.34 -0.5 138,305 + EX28DRAFT_4157 0.40 -0.2 138,458 + EX28DRAFT_4157 0.60 -0.2 138,458 + EX28DRAFT_4157 0.60 +0.4 138,543 - EX28DRAFT_4157 0.71 +0.8 138,589 + EX28DRAFT_4157 0.77 -0.6 138,590 - EX28DRAFT_4157 0.78 -1.0 138,636 - EX28DRAFT_4157 0.84 -0.3 138,636 - EX28DRAFT_4157 0.84 -0.6 138,647 + EX28DRAFT_4157 0.85 -0.8 138,647 + EX28DRAFT_4157 0.85 +0.2 138,666 + EX28DRAFT_4157 0.88 -0.4 138,774 - -1.9 138,813 + -0.7 138,814 - +0.4 139,051 - EX28DRAFT_4158 0.30 -0.8 139,051 - EX28DRAFT_4158 0.30 -1.1 139,101 + EX28DRAFT_4158 0.35 -1.4 139,777 + +1.1 139,777 + +0.4 139,778 - +0.2 139,778 - -0.5 139,778 - -0.1 139,903 + -0.1 139,969 + EX28DRAFT_4159 0.39 +0.9 139,970 - EX28DRAFT_4159 0.40 +0.2 139,975 + EX28DRAFT_4159 0.43 +2.4 139,976 - EX28DRAFT_4159 0.43 -1.2 139,976 - EX28DRAFT_4159 0.43 +1.3 139,976 - EX28DRAFT_4159 0.43 -2.4 139,986 - EX28DRAFT_4159 0.49 -0.5 140,002 - EX28DRAFT_4159 0.58 +1.1 140,088 + -0.7 140,106 + +0.4 140,148 - +0.6 140,209 + -0.0 140,210 - +0.3 140,210 - +0.1 140,303 - +1.0 140,303 - -0.6 140,447 + EX28DRAFT_4160 0.17 +0.2 140,448 - EX28DRAFT_4160 0.17 +0.4 140,448 - EX28DRAFT_4160 0.17 -1.3 140,599 - EX28DRAFT_4160 0.28 -0.2
Or see this region's nucleotide sequence