Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4154 and EX28DRAFT_4155 are separated by 67 nucleotides EX28DRAFT_4155 and EX28DRAFT_4156 are separated by 41 nucleotides EX28DRAFT_4156 and EX28DRAFT_4157 overlap by 8 nucleotides
EX28DRAFT_4154: EX28DRAFT_4154 - Uncharacterized protein conserved in bacteria, at 135,091 to 136,407
_4154
EX28DRAFT_4155: EX28DRAFT_4155 - DNA adenine methylase (dam), at 136,475 to 137,287
_4155
EX28DRAFT_4156: EX28DRAFT_4156 - ribulose-phosphate 3-epimerase, at 137,329 to 138,006
_4156
EX28DRAFT_4157: EX28DRAFT_4157 - 2-phosphoglycolate phosphatase, prokaryotic, at 137,999 to 138,760
_4157
Position (kb)
136
137
138 Strain fitness (log2 ratio)
-2
-1
0
1 at 135.671 kb on + strand, within EX28DRAFT_4154 at 135.867 kb on + strand, within EX28DRAFT_4154 at 135.867 kb on + strand, within EX28DRAFT_4154 at 136.162 kb on + strand, within EX28DRAFT_4154 at 136.192 kb on + strand, within EX28DRAFT_4154 at 136.192 kb on + strand, within EX28DRAFT_4154 at 136.276 kb on + strand at 136.276 kb on + strand at 136.297 kb on + strand at 136.297 kb on + strand at 136.300 kb on - strand at 136.300 kb on - strand at 136.406 kb on - strand at 136.459 kb on + strand at 136.459 kb on + strand at 136.537 kb on + strand at 136.624 kb on + strand, within EX28DRAFT_4155 at 136.847 kb on + strand, within EX28DRAFT_4155 at 136.979 kb on - strand, within EX28DRAFT_4155 at 137.009 kb on + strand, within EX28DRAFT_4155 at 137.171 kb on - strand, within EX28DRAFT_4155 at 137.183 kb on + strand, within EX28DRAFT_4155 at 137.222 kb on - strand at 137.228 kb on - strand at 137.495 kb on + strand, within EX28DRAFT_4156 at 137.499 kb on + strand, within EX28DRAFT_4156 at 137.710 kb on + strand, within EX28DRAFT_4156 at 137.993 kb on + strand at 137.993 kb on + strand at 138.004 kb on + strand at 138.099 kb on - strand, within EX28DRAFT_4157 at 138.099 kb on - strand, within EX28DRAFT_4157 at 138.138 kb on + strand, within EX28DRAFT_4157 at 138.138 kb on + strand, within EX28DRAFT_4157 at 138.139 kb on - strand, within EX28DRAFT_4157 at 138.259 kb on + strand, within EX28DRAFT_4157
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 135,671 + EX28DRAFT_4154 0.44 +0.1 135,867 + EX28DRAFT_4154 0.59 +0.8 135,867 + EX28DRAFT_4154 0.59 +0.0 136,162 + EX28DRAFT_4154 0.81 +0.3 136,192 + EX28DRAFT_4154 0.84 +0.1 136,192 + EX28DRAFT_4154 0.84 +0.4 136,276 + -0.4 136,276 + +0.2 136,297 + -0.1 136,297 + -0.3 136,300 - +0.8 136,300 - +0.2 136,406 - +0.3 136,459 + +0.1 136,459 + +0.4 136,537 + -2.1 136,624 + EX28DRAFT_4155 0.18 -0.9 136,847 + EX28DRAFT_4155 0.46 -0.4 136,979 - EX28DRAFT_4155 0.62 +0.2 137,009 + EX28DRAFT_4155 0.66 +0.1 137,171 - EX28DRAFT_4155 0.86 -0.2 137,183 + EX28DRAFT_4155 0.87 -2.5 137,222 - -1.3 137,228 - -1.4 137,495 + EX28DRAFT_4156 0.24 +0.4 137,499 + EX28DRAFT_4156 0.25 -0.4 137,710 + EX28DRAFT_4156 0.56 -2.0 137,993 + +0.6 137,993 + -0.2 138,004 + +0.7 138,099 - EX28DRAFT_4157 0.13 -0.3 138,099 - EX28DRAFT_4157 0.13 -1.3 138,138 + EX28DRAFT_4157 0.18 -0.0 138,138 + EX28DRAFT_4157 0.18 -0.3 138,139 - EX28DRAFT_4157 0.18 -0.5 138,259 + EX28DRAFT_4157 0.34 -0.5
Or see this region's nucleotide sequence