Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3963 and EX28DRAFT_3964 are separated by 84 nucleotides EX28DRAFT_3964 and EX28DRAFT_3965 are separated by 204 nucleotides
EX28DRAFT_3963: EX28DRAFT_3963 - Cellulase (glycosyl hydrolase family 5), at 170,894 to 171,982
_3963
EX28DRAFT_3964: EX28DRAFT_3964 - D-serine deaminase transcriptional activator, at 172,067 to 172,993
_3964
EX28DRAFT_3965: EX28DRAFT_3965 - gluconate transporter, at 173,198 to 174,535
_3965
Position (kb) 172
173 Strain fitness (log2 ratio) -1
0
1
2 at 171.283 kb on + strand, within EX28DRAFT_3963 at 171.332 kb on - strand, within EX28DRAFT_3963 at 171.364 kb on + strand, within EX28DRAFT_3963 at 171.364 kb on + strand, within EX28DRAFT_3963 at 171.365 kb on - strand, within EX28DRAFT_3963 at 171.493 kb on + strand, within EX28DRAFT_3963 at 171.508 kb on - strand, within EX28DRAFT_3963 at 171.518 kb on + strand, within EX28DRAFT_3963 at 171.559 kb on - strand, within EX28DRAFT_3963 at 171.665 kb on - strand, within EX28DRAFT_3963 at 171.666 kb on + strand, within EX28DRAFT_3963 at 171.667 kb on - strand, within EX28DRAFT_3963 at 171.706 kb on - strand, within EX28DRAFT_3963 at 171.722 kb on + strand, within EX28DRAFT_3963 at 171.967 kb on + strand at 171.968 kb on - strand at 171.996 kb on + strand at 172.010 kb on + strand at 172.016 kb on + strand at 172.017 kb on - strand at 172.044 kb on + strand at 172.057 kb on + strand at 172.057 kb on + strand at 172.060 kb on + strand at 172.060 kb on + strand at 172.107 kb on - strand at 172.141 kb on + strand at 172.141 kb on + strand at 172.142 kb on - strand at 172.142 kb on - strand at 172.142 kb on - strand at 172.142 kb on - strand at 172.143 kb on + strand at 172.143 kb on + strand at 172.143 kb on + strand at 172.143 kb on + strand at 172.143 kb on + strand at 172.144 kb on - strand at 172.144 kb on - strand at 172.144 kb on - strand at 172.146 kb on + strand at 172.295 kb on - strand, within EX28DRAFT_3964 at 172.450 kb on - strand, within EX28DRAFT_3964 at 172.519 kb on + strand, within EX28DRAFT_3964 at 172.932 kb on + strand at 173.027 kb on + strand at 173.129 kb on + strand at 173.181 kb on + strand at 173.181 kb on + strand at 173.181 kb on + strand at 173.182 kb on - strand at 173.182 kb on - strand at 173.197 kb on + strand at 173.510 kb on - strand, within EX28DRAFT_3965 at 173.704 kb on + strand, within EX28DRAFT_3965 at 173.704 kb on + strand, within EX28DRAFT_3965 at 173.709 kb on + strand, within EX28DRAFT_3965 at 173.751 kb on + strand, within EX28DRAFT_3965 at 173.752 kb on - strand, within EX28DRAFT_3965 at 173.973 kb on - strand, within EX28DRAFT_3965 at 173.975 kb on - strand, within EX28DRAFT_3965
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 171,283 + EX28DRAFT_3963 0.36 -1.5 171,332 - EX28DRAFT_3963 0.40 +0.1 171,364 + EX28DRAFT_3963 0.43 -0.1 171,364 + EX28DRAFT_3963 0.43 +1.1 171,365 - EX28DRAFT_3963 0.43 +2.4 171,493 + EX28DRAFT_3963 0.55 -1.2 171,508 - EX28DRAFT_3963 0.56 -0.4 171,518 + EX28DRAFT_3963 0.57 +0.9 171,559 - EX28DRAFT_3963 0.61 -0.1 171,665 - EX28DRAFT_3963 0.71 +0.3 171,666 + EX28DRAFT_3963 0.71 +0.1 171,667 - EX28DRAFT_3963 0.71 +0.4 171,706 - EX28DRAFT_3963 0.75 -0.4 171,722 + EX28DRAFT_3963 0.76 +0.2 171,967 + -0.3 171,968 - -0.6 171,996 + -0.5 172,010 + -0.3 172,016 + +0.4 172,017 - +1.2 172,044 + +0.6 172,057 + +0.0 172,057 + -0.1 172,060 + -0.4 172,060 + -0.3 172,107 - -0.1 172,141 + -0.6 172,141 + +0.2 172,142 - -1.2 172,142 - +0.1 172,142 - -0.3 172,142 - +0.4 172,143 + +0.1 172,143 + -0.3 172,143 + -0.1 172,143 + +0.1 172,143 + +0.3 172,144 - -0.1 172,144 - +0.4 172,144 - +0.9 172,146 + +0.7 172,295 - EX28DRAFT_3964 0.25 -0.6 172,450 - EX28DRAFT_3964 0.41 -0.7 172,519 + EX28DRAFT_3964 0.49 -0.6 172,932 + +0.4 173,027 + +0.1 173,129 + -0.4 173,181 + -0.5 173,181 + +0.3 173,181 + -0.5 173,182 - -0.0 173,182 - +0.6 173,197 + +0.3 173,510 - EX28DRAFT_3965 0.23 +0.2 173,704 + EX28DRAFT_3965 0.38 +0.4 173,704 + EX28DRAFT_3965 0.38 +1.8 173,709 + EX28DRAFT_3965 0.38 +0.2 173,751 + EX28DRAFT_3965 0.41 -0.7 173,752 - EX28DRAFT_3965 0.41 +0.4 173,973 - EX28DRAFT_3965 0.58 -0.1 173,975 - EX28DRAFT_3965 0.58 +0.9
Or see this region's nucleotide sequence