Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3752 and EX28DRAFT_3753 are separated by 111 nucleotides EX28DRAFT_3753 and EX28DRAFT_3754 are separated by 32 nucleotides EX28DRAFT_3754 and EX28DRAFT_3755 are separated by 63 nucleotides
EX28DRAFT_3752: EX28DRAFT_3752 - Protein of unknown function (DUF3461), at 432,391 to 432,777
_3752
EX28DRAFT_3753: EX28DRAFT_3753 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 432,889 to 433,713
_3753
EX28DRAFT_3754: EX28DRAFT_3754 - UTP--GlnB (protein PII) uridylyltransferase, GlnD, at 433,746 to 436,421
_3754
EX28DRAFT_3755: EX28DRAFT_3755 - methionine aminopeptidase, type I, at 436,485 to 437,279
_3755
Position (kb) 433
434
435
436
437 Strain fitness (log2 ratio) -1
0
1 at 432.748 kb on - strand at 432.773 kb on + strand at 432.849 kb on + strand at 433.745 kb on - strand at 435.782 kb on - strand, within EX28DRAFT_3754 at 436.392 kb on - strand at 436.447 kb on - strand at 437.315 kb on - strand at 437.318 kb on - strand at 437.323 kb on - strand at 437.323 kb on - strand at 437.323 kb on - strand at 437.376 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 432,748 - -0.0 432,773 + +0.1 432,849 + -0.7 433,745 - +0.1 435,782 - EX28DRAFT_3754 0.76 -1.1 436,392 - -0.6 436,447 - -0.4 437,315 - +1.2 437,318 - +0.2 437,323 - +0.1 437,323 - +0.7 437,323 - +0.1 437,376 + +1.1
Or see this region's nucleotide sequence