Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3644 and EX28DRAFT_3645 are separated by 3 nucleotides EX28DRAFT_3645 and EX28DRAFT_3646 overlap by 4 nucleotides EX28DRAFT_3646 and EX28DRAFT_3647 overlap by 1 nucleotides
EX28DRAFT_3644: EX28DRAFT_3644 - Uncharacterized protein affecting Mg2+/Co2+ transport, at 306,179 to 306,556
_3644
EX28DRAFT_3645: EX28DRAFT_3645 - ribosomal RNA small subunit methyltransferase A, at 306,560 to 307,381
_3645
EX28DRAFT_3646: EX28DRAFT_3646 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 307,378 to 308,364
_3646
EX28DRAFT_3647: EX28DRAFT_3647 - periplasmic chaperone for outer membrane proteins SurA, at 308,364 to 309,650
_3647
Position (kb) 307
308
309 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 306.465 kb on + strand, within EX28DRAFT_3644 at 306.514 kb on - strand, within EX28DRAFT_3644 at 306.524 kb on + strand at 306.525 kb on - strand at 306.537 kb on - strand at 306.561 kb on + strand at 306.886 kb on - strand, within EX28DRAFT_3645 at 306.886 kb on - strand, within EX28DRAFT_3645 at 306.995 kb on - strand, within EX28DRAFT_3645 at 306.997 kb on - strand, within EX28DRAFT_3645 at 307.389 kb on + strand at 307.423 kb on - strand at 307.423 kb on - strand at 307.575 kb on - strand, within EX28DRAFT_3646 at 307.575 kb on - strand, within EX28DRAFT_3646 at 308.081 kb on + strand, within EX28DRAFT_3646 at 308.081 kb on + strand, within EX28DRAFT_3646 at 308.081 kb on + strand, within EX28DRAFT_3646 at 308.082 kb on - strand, within EX28DRAFT_3646 at 308.207 kb on - strand, within EX28DRAFT_3646 at 308.490 kb on - strand at 308.609 kb on + strand, within EX28DRAFT_3647 at 308.946 kb on - strand, within EX28DRAFT_3647 at 309.047 kb on + strand, within EX28DRAFT_3647 at 309.268 kb on + strand, within EX28DRAFT_3647 at 309.277 kb on + strand, within EX28DRAFT_3647 at 309.293 kb on - strand, within EX28DRAFT_3647 at 309.318 kb on + strand, within EX28DRAFT_3647 at 309.326 kb on + strand, within EX28DRAFT_3647 at 309.326 kb on + strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647 at 309.327 kb on - strand, within EX28DRAFT_3647
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 306,465 + EX28DRAFT_3644 0.76 +0.6 306,514 - EX28DRAFT_3644 0.89 +0.0 306,524 + -0.0 306,525 - -0.1 306,537 - +1.2 306,561 + -0.4 306,886 - EX28DRAFT_3645 0.40 -1.1 306,886 - EX28DRAFT_3645 0.40 +0.0 306,995 - EX28DRAFT_3645 0.53 -0.5 306,997 - EX28DRAFT_3645 0.53 -1.0 307,389 + +3.2 307,423 - -2.8 307,423 - -2.3 307,575 - EX28DRAFT_3646 0.20 -1.3 307,575 - EX28DRAFT_3646 0.20 -3.0 308,081 + EX28DRAFT_3646 0.71 -2.1 308,081 + EX28DRAFT_3646 0.71 -3.2 308,081 + EX28DRAFT_3646 0.71 -0.8 308,082 - EX28DRAFT_3646 0.71 -3.3 308,207 - EX28DRAFT_3646 0.84 -2.5 308,490 - -0.4 308,609 + EX28DRAFT_3647 0.19 -3.8 308,946 - EX28DRAFT_3647 0.45 +0.0 309,047 + EX28DRAFT_3647 0.53 -2.3 309,268 + EX28DRAFT_3647 0.70 -1.5 309,277 + EX28DRAFT_3647 0.71 -1.0 309,293 - EX28DRAFT_3647 0.72 -2.5 309,318 + EX28DRAFT_3647 0.74 -2.1 309,326 + EX28DRAFT_3647 0.75 -2.0 309,326 + EX28DRAFT_3647 0.75 -2.9 309,327 - EX28DRAFT_3647 0.75 -1.9 309,327 - EX28DRAFT_3647 0.75 +1.2 309,327 - EX28DRAFT_3647 0.75 +0.3 309,327 - EX28DRAFT_3647 0.75 +1.2
Or see this region's nucleotide sequence