Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3612 and EX28DRAFT_3613 are separated by 218 nucleotides EX28DRAFT_3613 and EX28DRAFT_3614 are separated by 24 nucleotides EX28DRAFT_3614 and EX28DRAFT_3615 are separated by 1 nucleotides EX28DRAFT_3615 and EX28DRAFT_3616 are separated by 3 nucleotides
EX28DRAFT_3612: EX28DRAFT_3612 - RNA methyltransferase, TrmH family, group 1, at 271,320 to 272,006
_3612
EX28DRAFT_3613: EX28DRAFT_3613 - RNA, at 272,225 to 272,342
_3613
EX28DRAFT_3614: EX28DRAFT_3614 - aspartate kinase (EC 2.7.2.4), at 272,367 to 274,829
_3614
EX28DRAFT_3615: EX28DRAFT_3615 - homoserine kinase (EC 2.7.1.39), at 274,831 to 275,760
_3615
EX28DRAFT_3616: EX28DRAFT_3616 - threonine synthase, at 275,764 to 277,050
_3616
Position (kb)
272
273
274
275 Strain fitness (log2 ratio)
-2
-1
0
1 at 271.412 kb on - strand, within EX28DRAFT_3612 at 271.412 kb on - strand, within EX28DRAFT_3612 at 271.581 kb on + strand, within EX28DRAFT_3612 at 271.581 kb on + strand, within EX28DRAFT_3612 at 271.751 kb on - strand, within EX28DRAFT_3612 at 271.751 kb on - strand, within EX28DRAFT_3612 at 271.770 kb on + strand, within EX28DRAFT_3612 at 271.770 kb on + strand, within EX28DRAFT_3612 at 271.771 kb on - strand, within EX28DRAFT_3612 at 271.783 kb on + strand, within EX28DRAFT_3612 at 271.793 kb on - strand, within EX28DRAFT_3612 at 271.793 kb on - strand, within EX28DRAFT_3612 at 271.795 kb on + strand, within EX28DRAFT_3612 at 271.796 kb on - strand, within EX28DRAFT_3612 at 271.874 kb on - strand, within EX28DRAFT_3612 at 271.874 kb on - strand, within EX28DRAFT_3612 at 272.146 kb on + strand at 272.192 kb on + strand at 272.193 kb on - strand at 272.206 kb on + strand at 272.244 kb on + strand, within EX28DRAFT_3613 at 272.245 kb on - strand, within EX28DRAFT_3613 at 272.247 kb on + strand, within EX28DRAFT_3613 at 272.247 kb on + strand, within EX28DRAFT_3613 at 272.247 kb on + strand, within EX28DRAFT_3613 at 272.248 kb on - strand, within EX28DRAFT_3613 at 272.271 kb on + strand, within EX28DRAFT_3613 at 272.320 kb on + strand, within EX28DRAFT_3613 at 272.321 kb on - strand, within EX28DRAFT_3613 at 272.321 kb on - strand, within EX28DRAFT_3613 at 272.360 kb on + strand at 272.391 kb on - strand at 272.574 kb on - strand at 272.757 kb on - strand, within EX28DRAFT_3614 at 272.845 kb on - strand, within EX28DRAFT_3614 at 273.040 kb on + strand, within EX28DRAFT_3614 at 273.064 kb on - strand, within EX28DRAFT_3614 at 273.064 kb on - strand, within EX28DRAFT_3614 at 273.142 kb on - strand, within EX28DRAFT_3614 at 273.381 kb on - strand, within EX28DRAFT_3614 at 273.381 kb on - strand, within EX28DRAFT_3614 at 273.396 kb on - strand, within EX28DRAFT_3614 at 273.396 kb on - strand, within EX28DRAFT_3614 at 273.396 kb on - strand, within EX28DRAFT_3614 at 273.645 kb on - strand, within EX28DRAFT_3614 at 273.852 kb on - strand, within EX28DRAFT_3614 at 274.129 kb on - strand, within EX28DRAFT_3614 at 274.132 kb on - strand, within EX28DRAFT_3614 at 274.180 kb on - strand, within EX28DRAFT_3614 at 274.292 kb on + strand, within EX28DRAFT_3614 at 274.293 kb on - strand, within EX28DRAFT_3614 at 274.415 kb on - strand, within EX28DRAFT_3614 at 274.587 kb on - strand at 274.600 kb on - strand at 274.707 kb on + strand at 274.717 kb on - strand at 274.720 kb on - strand at 274.820 kb on - strand at 275.019 kb on + strand, within EX28DRAFT_3615 at 275.683 kb on - strand at 275.719 kb on - strand at 275.758 kb on + strand at 275.758 kb on + strand at 275.762 kb on + strand at 275.763 kb on - strand at 275.763 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.774 kb on - strand at 275.782 kb on - strand at 275.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 271,412 - EX28DRAFT_3612 0.13 -1.3 271,412 - EX28DRAFT_3612 0.13 +0.4 271,581 + EX28DRAFT_3612 0.38 +0.6 271,581 + EX28DRAFT_3612 0.38 -0.2 271,751 - EX28DRAFT_3612 0.63 +0.5 271,751 - EX28DRAFT_3612 0.63 -0.4 271,770 + EX28DRAFT_3612 0.66 -0.8 271,770 + EX28DRAFT_3612 0.66 +0.9 271,771 - EX28DRAFT_3612 0.66 +0.3 271,783 + EX28DRAFT_3612 0.67 -0.4 271,793 - EX28DRAFT_3612 0.69 +0.1 271,793 - EX28DRAFT_3612 0.69 +1.2 271,795 + EX28DRAFT_3612 0.69 -0.3 271,796 - EX28DRAFT_3612 0.69 +1.1 271,874 - EX28DRAFT_3612 0.81 -0.7 271,874 - EX28DRAFT_3612 0.81 -0.6 272,146 + -0.3 272,192 + -0.6 272,193 - -0.5 272,206 + +0.2 272,244 + EX28DRAFT_3613 0.16 +0.1 272,245 - EX28DRAFT_3613 0.17 -1.0 272,247 + EX28DRAFT_3613 0.19 -0.2 272,247 + EX28DRAFT_3613 0.19 -0.6 272,247 + EX28DRAFT_3613 0.19 -0.7 272,248 - EX28DRAFT_3613 0.19 -1.2 272,271 + EX28DRAFT_3613 0.39 -0.5 272,320 + EX28DRAFT_3613 0.81 -1.8 272,321 - EX28DRAFT_3613 0.81 -0.3 272,321 - EX28DRAFT_3613 0.81 -0.6 272,360 + +0.4 272,391 - -1.0 272,574 - +0.2 272,757 - EX28DRAFT_3614 0.16 -0.4 272,845 - EX28DRAFT_3614 0.19 -0.8 273,040 + EX28DRAFT_3614 0.27 +0.1 273,064 - EX28DRAFT_3614 0.28 -1.4 273,064 - EX28DRAFT_3614 0.28 -0.5 273,142 - EX28DRAFT_3614 0.31 -0.2 273,381 - EX28DRAFT_3614 0.41 -1.7 273,381 - EX28DRAFT_3614 0.41 -1.2 273,396 - EX28DRAFT_3614 0.42 +0.7 273,396 - EX28DRAFT_3614 0.42 -1.1 273,396 - EX28DRAFT_3614 0.42 -1.1 273,645 - EX28DRAFT_3614 0.52 -0.6 273,852 - EX28DRAFT_3614 0.60 -0.4 274,129 - EX28DRAFT_3614 0.72 -1.2 274,132 - EX28DRAFT_3614 0.72 -0.3 274,180 - EX28DRAFT_3614 0.74 +0.1 274,292 + EX28DRAFT_3614 0.78 -0.9 274,293 - EX28DRAFT_3614 0.78 -1.8 274,415 - EX28DRAFT_3614 0.83 -0.7 274,587 - -0.2 274,600 - -1.3 274,707 + -1.7 274,717 - +0.6 274,720 - -0.8 274,820 - -0.9 275,019 + EX28DRAFT_3615 0.20 +0.5 275,683 - +0.7 275,719 - -1.9 275,758 + -1.8 275,758 + -1.0 275,762 + -2.1 275,763 - -0.6 275,763 - -2.4 275,774 - -2.1 275,774 - -1.1 275,774 - +0.7 275,782 - -1.0 275,790 + -0.0
Or see this region's nucleotide sequence