Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3599 and EX28DRAFT_3600 are separated by 108 nucleotides EX28DRAFT_3600 and EX28DRAFT_3601 are separated by 91 nucleotides
EX28DRAFT_3599: EX28DRAFT_3599 - zinc-binding alcohol dehydrogenase family protein, at 257,277 to 258,278
_3599
EX28DRAFT_3600: EX28DRAFT_3600 - Transcriptional regulator, at 258,387 to 259,286
_3600
EX28DRAFT_3601: EX28DRAFT_3601 - ATP-binding cassette protein, ChvD family, at 259,378 to 261,045
_3601
Position (kb) 258
259
260 Strain fitness (log2 ratio) -1
0
1
2 at 257.462 kb on - strand, within EX28DRAFT_3599 at 257.476 kb on + strand, within EX28DRAFT_3599 at 257.480 kb on + strand, within EX28DRAFT_3599 at 257.561 kb on + strand, within EX28DRAFT_3599 at 257.561 kb on + strand, within EX28DRAFT_3599 at 257.632 kb on + strand, within EX28DRAFT_3599 at 257.633 kb on - strand, within EX28DRAFT_3599 at 257.694 kb on + strand, within EX28DRAFT_3599 at 258.027 kb on - strand, within EX28DRAFT_3599 at 258.027 kb on - strand, within EX28DRAFT_3599 at 258.180 kb on + strand at 258.319 kb on + strand at 258.319 kb on + strand at 258.320 kb on - strand at 258.321 kb on + strand at 258.322 kb on - strand at 258.393 kb on - strand at 258.812 kb on - strand, within EX28DRAFT_3600 at 258.812 kb on - strand, within EX28DRAFT_3600 at 258.960 kb on - strand, within EX28DRAFT_3600 at 259.052 kb on - strand, within EX28DRAFT_3600 at 259.053 kb on + strand, within EX28DRAFT_3600 at 259.053 kb on + strand, within EX28DRAFT_3600 at 259.118 kb on + strand, within EX28DRAFT_3600 at 259.173 kb on - strand, within EX28DRAFT_3600 at 259.230 kb on + strand at 259.230 kb on + strand at 259.230 kb on + strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.231 kb on - strand at 259.272 kb on + strand at 259.272 kb on + strand at 259.389 kb on + strand at 259.389 kb on + strand at 259.390 kb on - strand at 259.397 kb on + strand at 259.401 kb on + strand at 259.401 kb on + strand at 259.401 kb on + strand at 259.401 kb on + strand at 259.401 kb on + strand at 259.402 kb on - strand at 259.402 kb on - strand at 259.402 kb on - strand at 259.404 kb on + strand at 259.448 kb on + strand at 259.458 kb on - strand at 259.458 kb on - strand at 259.470 kb on + strand at 259.470 kb on + strand at 259.471 kb on - strand at 259.471 kb on - strand at 259.558 kb on - strand, within EX28DRAFT_3601 at 259.767 kb on - strand, within EX28DRAFT_3601 at 259.767 kb on - strand, within EX28DRAFT_3601 at 259.962 kb on + strand, within EX28DRAFT_3601 at 260.055 kb on + strand, within EX28DRAFT_3601 at 260.056 kb on - strand, within EX28DRAFT_3601 at 260.191 kb on - strand, within EX28DRAFT_3601 at 260.247 kb on + strand, within EX28DRAFT_3601 at 260.247 kb on + strand, within EX28DRAFT_3601
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 257,462 - EX28DRAFT_3599 0.18 +0.4 257,476 + EX28DRAFT_3599 0.20 -0.4 257,480 + EX28DRAFT_3599 0.20 -0.1 257,561 + EX28DRAFT_3599 0.28 -0.2 257,561 + EX28DRAFT_3599 0.28 +0.1 257,632 + EX28DRAFT_3599 0.35 +0.3 257,633 - EX28DRAFT_3599 0.36 +0.2 257,694 + EX28DRAFT_3599 0.42 +0.0 258,027 - EX28DRAFT_3599 0.75 +0.4 258,027 - EX28DRAFT_3599 0.75 -0.6 258,180 + +0.5 258,319 + +0.2 258,319 + +0.1 258,320 - -0.9 258,321 + -0.0 258,322 - -0.7 258,393 - -0.3 258,812 - EX28DRAFT_3600 0.47 +1.2 258,812 - EX28DRAFT_3600 0.47 +1.5 258,960 - EX28DRAFT_3600 0.64 +0.2 259,052 - EX28DRAFT_3600 0.74 -0.9 259,053 + EX28DRAFT_3600 0.74 -0.4 259,053 + EX28DRAFT_3600 0.74 -0.7 259,118 + EX28DRAFT_3600 0.81 -0.4 259,173 - EX28DRAFT_3600 0.87 -0.6 259,230 + -0.3 259,230 + +0.4 259,230 + -0.4 259,231 - +1.2 259,231 - -0.4 259,231 - +0.2 259,231 - -0.4 259,231 - -0.5 259,231 - +0.2 259,231 - +0.2 259,231 - -0.4 259,231 - +0.1 259,231 - +0.3 259,272 + -0.2 259,272 + -0.0 259,389 + -0.8 259,389 + -0.1 259,390 - +0.1 259,397 + +0.2 259,401 + -0.2 259,401 + +0.5 259,401 + +1.0 259,401 + +0.1 259,401 + -0.4 259,402 - -0.0 259,402 - -0.4 259,402 - -0.5 259,404 + +0.1 259,448 + -0.6 259,458 - -0.5 259,458 - -0.4 259,470 + -1.5 259,470 + -0.8 259,471 - -0.1 259,471 - +2.2 259,558 - EX28DRAFT_3601 0.11 +1.8 259,767 - EX28DRAFT_3601 0.23 -0.8 259,767 - EX28DRAFT_3601 0.23 -0.5 259,962 + EX28DRAFT_3601 0.35 -0.2 260,055 + EX28DRAFT_3601 0.41 -0.3 260,056 - EX28DRAFT_3601 0.41 -0.1 260,191 - EX28DRAFT_3601 0.49 +0.5 260,247 + EX28DRAFT_3601 0.52 +0.5 260,247 + EX28DRAFT_3601 0.52 +0.1
Or see this region's nucleotide sequence