Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3596 and EX28DRAFT_3597 are separated by 74 nucleotides EX28DRAFT_3597 and EX28DRAFT_3598 are separated by 37 nucleotides
EX28DRAFT_3596: EX28DRAFT_3596 - phosphoserine phosphatase SerB, at 253,481 to 254,449
_3596
EX28DRAFT_3597: EX28DRAFT_3597 - DNA repair protein RadA, at 254,524 to 255,909
_3597
EX28DRAFT_3598: EX28DRAFT_3598 - nicotinamide-nucleotide adenylyltransferase, NadR type, at 255,947 to 257,179
_3598
Position (kb) 254
255
256 Strain fitness (log2 ratio) -1
0
1 at 254.448 kb on - strand at 254.557 kb on - strand at 254.848 kb on - strand, within EX28DRAFT_3597 at 255.133 kb on + strand, within EX28DRAFT_3597 at 255.726 kb on - strand, within EX28DRAFT_3597 at 255.772 kb on - strand at 255.858 kb on - strand at 255.906 kb on - strand at 255.906 kb on - strand at 255.907 kb on + strand at 255.912 kb on - strand at 255.912 kb on - strand at 255.941 kb on + strand at 255.942 kb on - strand at 255.946 kb on + strand at 255.947 kb on - strand at 255.947 kb on - strand at 255.947 kb on - strand at 255.963 kb on - strand at 256.130 kb on - strand, within EX28DRAFT_3598 at 256.186 kb on + strand, within EX28DRAFT_3598 at 256.186 kb on + strand, within EX28DRAFT_3598 at 256.186 kb on + strand, within EX28DRAFT_3598 at 256.186 kb on + strand, within EX28DRAFT_3598 at 256.187 kb on - strand, within EX28DRAFT_3598 at 256.187 kb on - strand, within EX28DRAFT_3598 at 256.187 kb on - strand, within EX28DRAFT_3598 at 256.187 kb on - strand, within EX28DRAFT_3598 at 256.187 kb on - strand, within EX28DRAFT_3598 at 256.190 kb on + strand, within EX28DRAFT_3598 at 256.190 kb on + strand, within EX28DRAFT_3598 at 256.191 kb on - strand, within EX28DRAFT_3598 at 256.191 kb on - strand, within EX28DRAFT_3598 at 256.191 kb on - strand, within EX28DRAFT_3598 at 256.339 kb on + strand, within EX28DRAFT_3598 at 256.339 kb on + strand, within EX28DRAFT_3598 at 256.340 kb on - strand, within EX28DRAFT_3598 at 256.340 kb on - strand, within EX28DRAFT_3598 at 256.343 kb on + strand, within EX28DRAFT_3598 at 256.439 kb on - strand, within EX28DRAFT_3598 at 256.462 kb on + strand, within EX28DRAFT_3598 at 256.472 kb on + strand, within EX28DRAFT_3598 at 256.472 kb on + strand, within EX28DRAFT_3598 at 256.472 kb on + strand, within EX28DRAFT_3598 at 256.472 kb on + strand, within EX28DRAFT_3598 at 256.473 kb on - strand, within EX28DRAFT_3598 at 256.473 kb on - strand, within EX28DRAFT_3598 at 256.473 kb on - strand, within EX28DRAFT_3598 at 256.556 kb on - strand, within EX28DRAFT_3598 at 256.607 kb on + strand, within EX28DRAFT_3598 at 256.607 kb on + strand, within EX28DRAFT_3598 at 256.608 kb on - strand, within EX28DRAFT_3598 at 256.608 kb on - strand, within EX28DRAFT_3598 at 256.647 kb on + strand, within EX28DRAFT_3598 at 256.651 kb on + strand, within EX28DRAFT_3598 at 256.651 kb on + strand, within EX28DRAFT_3598 at 256.651 kb on + strand, within EX28DRAFT_3598 at 256.652 kb on - strand, within EX28DRAFT_3598 at 256.652 kb on - strand, within EX28DRAFT_3598 at 256.733 kb on + strand, within EX28DRAFT_3598 at 256.745 kb on + strand, within EX28DRAFT_3598 at 256.745 kb on + strand, within EX28DRAFT_3598 at 256.746 kb on - strand, within EX28DRAFT_3598 at 256.746 kb on - strand, within EX28DRAFT_3598 at 256.796 kb on + strand, within EX28DRAFT_3598 at 256.827 kb on - strand, within EX28DRAFT_3598 at 256.827 kb on - strand, within EX28DRAFT_3598
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 254,448 - -1.6 254,557 - +0.2 254,848 - EX28DRAFT_3597 0.23 +0.3 255,133 + EX28DRAFT_3597 0.44 -1.4 255,726 - EX28DRAFT_3597 0.87 -1.0 255,772 - +0.3 255,858 - -0.4 255,906 - -0.2 255,906 - -0.3 255,907 + -0.2 255,912 - +0.1 255,912 - -0.3 255,941 + -0.3 255,942 - -0.1 255,946 + -0.1 255,947 - -0.4 255,947 - -0.2 255,947 - -0.4 255,963 - +0.4 256,130 - EX28DRAFT_3598 0.15 -0.7 256,186 + EX28DRAFT_3598 0.19 -0.3 256,186 + EX28DRAFT_3598 0.19 -0.5 256,186 + EX28DRAFT_3598 0.19 -0.6 256,186 + EX28DRAFT_3598 0.19 -0.2 256,187 - EX28DRAFT_3598 0.19 -0.5 256,187 - EX28DRAFT_3598 0.19 +0.1 256,187 - EX28DRAFT_3598 0.19 -0.2 256,187 - EX28DRAFT_3598 0.19 +0.3 256,187 - EX28DRAFT_3598 0.19 -0.1 256,190 + EX28DRAFT_3598 0.20 -1.6 256,190 + EX28DRAFT_3598 0.20 +0.5 256,191 - EX28DRAFT_3598 0.20 -1.2 256,191 - EX28DRAFT_3598 0.20 -0.1 256,191 - EX28DRAFT_3598 0.20 -0.3 256,339 + EX28DRAFT_3598 0.32 -0.3 256,339 + EX28DRAFT_3598 0.32 -0.1 256,340 - EX28DRAFT_3598 0.32 +0.8 256,340 - EX28DRAFT_3598 0.32 +0.6 256,343 + EX28DRAFT_3598 0.32 -0.4 256,439 - EX28DRAFT_3598 0.40 +0.0 256,462 + EX28DRAFT_3598 0.42 -1.1 256,472 + EX28DRAFT_3598 0.43 +0.8 256,472 + EX28DRAFT_3598 0.43 -0.0 256,472 + EX28DRAFT_3598 0.43 +1.4 256,472 + EX28DRAFT_3598 0.43 -1.3 256,473 - EX28DRAFT_3598 0.43 +0.4 256,473 - EX28DRAFT_3598 0.43 -0.4 256,473 - EX28DRAFT_3598 0.43 +0.3 256,556 - EX28DRAFT_3598 0.49 -0.2 256,607 + EX28DRAFT_3598 0.54 -0.1 256,607 + EX28DRAFT_3598 0.54 +0.2 256,608 - EX28DRAFT_3598 0.54 +0.2 256,608 - EX28DRAFT_3598 0.54 -0.3 256,647 + EX28DRAFT_3598 0.57 -0.4 256,651 + EX28DRAFT_3598 0.57 -1.0 256,651 + EX28DRAFT_3598 0.57 -0.1 256,651 + EX28DRAFT_3598 0.57 +0.2 256,652 - EX28DRAFT_3598 0.57 -0.8 256,652 - EX28DRAFT_3598 0.57 +0.0 256,733 + EX28DRAFT_3598 0.64 -0.5 256,745 + EX28DRAFT_3598 0.65 +0.2 256,745 + EX28DRAFT_3598 0.65 +0.4 256,746 - EX28DRAFT_3598 0.65 +0.2 256,746 - EX28DRAFT_3598 0.65 -0.3 256,796 + EX28DRAFT_3598 0.69 -0.3 256,827 - EX28DRAFT_3598 0.71 +0.2 256,827 - EX28DRAFT_3598 0.71 +0.6
Or see this region's nucleotide sequence